Journal: Frontiers in Physiology

Article Title: TRPV4 Complexes With the Na + /Ca 2+ Exchanger and IP 3 Receptor 1 to Regulate Local Intracellular Calcium and Tracheal Tension in Mice

doi: 10.3389/fphys.2019.01471

Figure Lengend Snippet: Effects of Ca 2+ -activated K + channel inhibitors on tracheal rings precontracted with a TRPV4 activator. Compared with that in the control groups, the carbachol precontracted-tracheal ring relaxation-induced by GSK1016790A was not significantly reduced by pretreatment with the large-, intermediate-, and small-conductance Ca 2+ -activated K + channel inhibitors IbTX (50 μM), TRAM34 (10 μM), and apamin (1 μM). Data are shown as means ± SE; n = 5 mice.

Article Snippet: Iberiotoxin (IbTX), and TRAM34 were purchased from MedChemExpress.

Techniques: Control

Journal: Biomolecules

Article Title: Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage—Induced Mitochondrial Injury In Vitro

doi: 10.3390/biom9120825

Figure Lengend Snippet: Whole-cell patch-clamp does not detect BK channel-mediated current in plasma membranes of NRK cells. Cells were dialyzed with pipette solution containing 100 nM free calcium, and global K + currents were elicited by 10-mV steps from a holding potential of −70 mV to +80 mV. Representative recordings are shown from ( a , b ) control NRK cells and ( c , d ) NRK cells subjected to CS + RW. ( a ) and ( c ) show currents before and after 300 nM iberiotoxin (+IBTX), a BK channel blocker. ( b ) and ( d ) show currents before and after 1 μM NS11021, a BK channel opener. NRK cell recordings are representative of n = 5–6 for each condition. ( e , f ) As a positive control, rat renal vascular smooth muscle cells (VSMCs) were patch-clamped and exposed to NS11021 or iberiotoxin with the same protocol. Renal VSMC recordings are representative of n = 3 for each condition.

Article Snippet: Cells were subjected to the voltage protocol every 2 min to establish initial baseline amplitudes of voltage-activated outward K + current prior to superfusion of cells either with the BK channel activator, 1 μM NS11021 (Sigma, #SML0622), or with the BK channel blocker, 300 nM iberiotoxin (Tocris Bioscience, Bristol, UK, #1086).

Techniques: Patch Clamp, Clinical Proteomics, Transferring, Control, Positive Control

Journal: International Journal of Applied and Basic Medical Research

Article Title: Avanafil Inhibits the Contractility of the Isolated Caprine Detrusor Muscle

doi: 10.4103/ijabmr.IJABMR_339_18

Figure Lengend Snippet: Representative traces from the study: (a) Contractile effect of 80 mM potassium chloride before (left side) and after (right side) addition of 30 μM avanafil. (b) Contractile effect of 80 mM potassium chloride before (left side) and after (right side) addition of 100 nM iberiotoxin and 30 μM avanafil

Article Snippet: Iberiotoxin (Santa Cruz Biotechnology) was dissolved in double-distilled water to give a 4 μM stock solution.

Techniques:

Journal: Scientific reports

Article Title: Impaired BK Ca channel function in native vascular smooth muscle from humans with type 2 diabetes.

doi: 10.1038/s41598-017-14565-9

Figure Lengend Snippet: Figure 1. Adipose arteries from diabetic subjects exhibit blunted response to IBTx. (A) Exemplary image of small resistance adipose arteries used in this study. The image below shows an expanded view of the area in the green square. (B) Representative diameter recordings of pressurized (80 mmHg) adipose arteries from non-diabetic and diabetic patients before and after perfusion of 100 nM iberiotoxin or a Ca2+ free + nifedipine (1 μM) solution. (C) Summary of iberiotoxin-induced constriction in non-diabetic (n = 7 arteries, 4 subjects) and diabetic (n = 5 arteries, 5 subjects) adipose arteries pressurized to 80 mmHg. (D) Scatter/bar plot summarizing vascular tone at 80 mmHg in response to 60 mM extracellular K+ in non-diabetic (n = 8 arteries, 4 subjects) and diabetic (n = 6 arteries, 5 subjects) adipose arteries. *P < 0.05, Mann-Whitney test. Significance was compared between non-diabetic and diabetic datasets.

Article Snippet: Iberiotoxin was from Peptides International (Louisville, KY).

Techniques: MANN-WHITNEY

Journal:

Article Title: BK channel activation by NS-1619 is partially mediated by intracellular Ca 2+ release in smooth muscle cells of porcine coronary artery

doi: 10.1038/sj.bjp.0703885

Figure Lengend Snippet: Effects of NS-1619 on membrane potential (M.P.) in single smooth muscle cells of porcine coronary artery under current clamp mode. (A) Application of 30 μM NS-1619 hyperpolarized the myocyte by 12 mV. The NS-1619-induced hyperpolarization was abolished by 100 nM iberiotoxin (IbTx). (B) Summarized data obtained from experiments typically shown in (A). The number of myocytes used is given in parentheses. The statistical significance of the difference between three groups is indicated by **P<0.01.

Article Snippet: Pharmacological reagents were obtained from Sigma (St. Louis, U.S.A.) except for NS-1619 (1,3-dihydro-1-[2-hydroxy-5-(trifluoromethyl) phenyl]-5-(tr ifluoromethyl)-2H-be nzimidazol-2-one; Research Biochemicals International, Natick, U.S.A.), iberiotoxin (IbTx; Peptide Institute, Osaka, Japan), caffeine (Wako, Osaka, Japan), collagenase (Amano, Nagoya, Japan), HEPES, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA), indo-1, fluo-3 (Dojin, Kumamoto, Japan) and fura-2 (Molecular Probes, Eugene, U.S.A.).

Techniques: