Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: The scheme of the chronic GC exposure increases NLRP1 inflammasome via activation of BK channel. Chronic GC exposure activates NLRP1 inflammasome by upregulation and activation of BK channel and leading to low [K + ] i in the hippocampal neurons. BK channel inhibitor IbTx inhibits activation of NLRP1 inflammasome by blocking BK channel and increases [K + ] i , which decreased by DEX treatment

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Activation Assay, Blocking Assay

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of DEX and IbTx treatment on the BK currents in hippocampal neurons (whole-cell patch-clamp technology). a Representative traces of potassium currents induced by BK channel inhibitor IbTx. b Representative traces of potassium currents induced by DEX. c Representative traces of potassium currents induced by DEX and IbTx. d Statistical results showing recorded potassium currents were markedly inhibited by specific BK channel inhibitor IbTx. e Statistical results showing DEX increased BK currents. f Statistical results showing DEX failed to increase BK currents in the presence of IbTx. Results are expressed as mean ± SD, n = 5 or n = 4. # P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Patch Clamp

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of chronic DEX and IbTx treatment on mRNA expression of NLRP1and IL-1β in hippocampal neurons. a The results of DEX and IbTx treatment for 3 days on mRNA expression of NLRP1. b The results of DEX and IbTx treatment for 3 days on mRNA expression of IL-1β. Results are expressed as mean ± SD, n = 3. * P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effects of DEX and IbTx treatment on the [K + ] i in hippocampal neurons (Ion-selective electrode). The results showed that DEX (1, 5, and 10 μM) treatment for 2 h significantly decreased [K + ] i in hippocampal neurons. Compared with DEX 5 μM-treated group, IbTx significantly increased [K + ] i in presence of DEX. Results are expressed as mean ± SD, n = 3. ** P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of chronic DEX and IbTx treatment on the expression of BK in hippocampal neurons (immunoblot). a The results of DEX 5 μM treatment for 1, 3, and 5 days on expression of BK in hippocampal neurons. b The results of DEX and IbTx treatment for 3 days on the expression of BK in hippocampal neurons. Results are expressed as mean ± SD, n = 4. * P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of chronic DEX and IbTx treatment on hippocampal neurons damage and apoptosis. a The results of DEX and IbTx treatment for 3 days on LDH release in medium. b The results of DEX and IbTx treatment for 3 days on hippocampal neuron apoptosis (Hoechst 33258 staining, ×400). c The analysis of the percent of apoptosis in hippocampal neurons. Results are expressed as mean ± SD, LDH assay n = 3, Hoechst staining n = 4. * P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Staining, Lactate Dehydrogenase Assay

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of chronic DEX and IbTx treatment on MAP2 expression in hippocampal neurons. a The results of DEX and IbTx treatment for 5 days on MAP2 expression in hippocampal neurons (immunofluorescence, ×400). b The immunoblot results of DEX and IbTx treatment for 5 days on MAP2 expression and quantitative analysis of the expression of MAP2. Results are expressed as mean ± SD, immunofluorescence n = 3, immunoblot n = 4. ** P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Expressing, Immunofluorescence

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effect of chronic DEX and IbTx treatment on the release of IL-1β and IL-18 in the supernatants (ELISA). a The results of DEX and IbTx treatment for 3 days on the release of IL-1β. b The results of DEX and IbTx treatment for 3 days on the release of IL-18. Results are expressed as mean ± SD, n = 4. * P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Neuroinflammation

Article Title: Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage

doi: 10.1186/s12974-017-0911-9

Figure Lengend Snippet: Effects of chronic DEX and IbTx treatment on expressions of NLRP1, ASC, caspase-1, and IL-1β (immunoblot). a The results of DEX and IbTx treatment for 3 days on expression of NLRP1. b The results of DEX and IbTx treatment for 3 days on expression of ASC. c The results of DEX and IbTx treatment for 3 d on expression of caspase-1. d The results of DEX and IbTx treatment for 3 days on expression of IL-1β. Results are expressed as mean ± SD, n = 4. * P

Article Snippet: The hippocampal neurons were cultured for 5 days before being treated with dexamethasone (DEX) (Sigma, USA) or iberiotoxin (IbTx) (Sigma, USA), the BK channel inhibitor.

Techniques: Expressing