Journal: Diabetologia

Article Title: Proinsulin peptide promotes autoimmune diabetes in a novel HLA-DR3-DQ2-transgenic murine model of spontaneous disease

doi: 10.1007/s00125-019-04994-8

Figure Lengend Snippet: Adjuvanted priming with murine proinsulin-2 peptides promotes diabetes. ( a ) DR3DQ2×RIP-B7.1 mice aged 7–12 weeks were primed with 100 μg of murine proinsulin-2 (muPI2) peptides (6 male, 4 female mice), human GAD65 (huGAD65) protein (5 male, 3 female mice) or the 377-amino-acid C-terminal region of IA-2 (huIA-2 fragment) (7 male, 2 female mice), and a PBS only control (5 male, 3 female mice) in TiterMax Gold adjuvant s.c. on days 0 and 14, with 200 ng pertussis toxin administered i.p. on days 0 and 1 or 2. Mice were monitored weekly for diabetes up to 20 weeks post prime. The grey dashed line indicates the expected level of spontaneous diabetes in mice aged 12 weeks on day 0 of the experiments (based on the data in Fig. and normalised for sex). ** p < 0.01 for muPI2 peptides vs huGAD65, and *** p < 0.001 for muPI2 peptides vs both PBS and huIA-2 fragments (Mantel–Cox logrank test). ( b – e ) Blood glucose levels for each individual mouse in the group immunised with PBS ( b ), murine proinsulin-2 peptides ( c ), human GAD65 ( d ) or fragment of human IA-2 ( e ); key in ( b ) also applies to ( c–e )

Article Snippet: The 377-amino-acid C-terminal fragment of human IA-2 was produced by ProteoGenix (Schiltigheim, France).

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