Journal: Inflammation

Article Title: Targeting Cold-Inducible RNA-Binding Protein Attenuates Pancreatic Fibrosis by Suppressing Pyroptosis in Chronic Pancreatitis

doi: 10.1007/s10753-026-02490-x

Figure Lengend Snippet: Inflammation and pyroptosis were increased in CP mice. Cerulein-CP was induced by 6 IP injections of cerulein (50 µg/kg/body weight) twice a week for 10 weeks. L-arginine-CP was induced by 2 hourly IP injections of 4.0 g/kg L-arginine twice a week for 10 weeks. The animals were sacrificed at 10 weeks after the first injection of L-arginine or cerulein. Blood and tissue samples were collected. ( A-C ) Representative photos and quantitative of F4/80 and CD11b staining; ( D ) Serum IL-6 level; ( E ) Serum TNF-α level; ( F & G ) Western blot analysis and quantitative of the expression of NLRP3 in the pancreas; ( H ) Serum IL-18 level; ( I ) Serum IL-1β level; ( J ) Correlation analysis of serum IL-18 and Masson positive aera; ( K ) Correlation analysis of serum IL-1β and Masson positive aera; ( L ) Correlation analysis of serum CIRP and F4/80 positive cells; ( M ) Correlation analysis of serum CIRP and CD11b positive cells; ( N ) Correlation analysis of serum CIRP and F4/80 positive cells; ( O ) Correlation analysis of serum CIRP and CD11b positive cells; ( R ) Correlation analysis of serum CIRP and IL-18; ( S ) Correlation analysis of serum CIRP and IL-1β; ( T ) Correlation analysis of serum CIRP and serum IL-18 in CP patients; ( U ) Correlation analysis of serum CIRP and serum IL-1β in CP patients; ( V ) Correlation analysis of serum CIRP and serum HMGB1 in CP patients. Data are presented as mean ± SD ( n = 6 per group). Statistical analyses were performed using GraphPad Prism 8.0. Statistical significance was determined by t-test or one-way ANOVA with Tukey’s post-hoc test. Correlations were assessed using Spearman’s rank correlation coefficient. * P < 0.05 indicates a statistically significant difference. CP, chronic pancreatitis; CIRP, Cold-inducible RNA-binding protein; L-arg, L-arginine; Cer, Cerulein; IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; NLRP3, Nod-like receptor family protein 3; IL-18, interleukin 18; IL-1β, HMGB1, high mobility group protein B1; interleukin 1β

Article Snippet: In the other groups of experimental CP, normal saline (vehicle) or 5, 20 or 100 mg/kg NLRP3 inhibitor [ ] (S3680, Selleck, Inc. CN) was administered by intraperitoneal injection 2 h after the last injection of L-arginine or cerulein.

Techniques: Injection, Staining, Western Blot, Expressing, RNA Binding Assay

Journal: Inflammation

Article Title: Targeting Cold-Inducible RNA-Binding Protein Attenuates Pancreatic Fibrosis by Suppressing Pyroptosis in Chronic Pancreatitis

doi: 10.1007/s10753-026-02490-x

Figure Lengend Snippet: CIRP deficiency alleviates inflammation and pyroptosis in CP model mice. Cerulein-CP was induced by 6 IP injections of cerulein (50 µg/kg/body weight) twice a week for 10 weeks. L-arginine-CP was induced by 2 hourly IP injections of 4.0 g/kg L-arginine twice a week for 10 weeks. 8 mg/kg C23 was administered 2 h after the last L-arginine or cerulein injection each time. To determine the role of TLR4 in CIRP’s effect in CP, TAK-242, a specific TLR4 receptor inhibitor, were administered through intraperitoneal injection at 1 h after the last injection of L-arginine or cerulein each time. The animals were sacrificed 10 weeks after the first injection of L-arginine or cerulein. Blood and tissue samples were collected. ( A & B ) Western blot analysis and quantitative of the expression of NLRP3, Cleaved Caspase-1 and Cleaved Gasdermin D in the pancreas; ( C-E ) Representative photos and quantitative of F4/80 and CD11b staining; ( F ) Serum IL-1β level; ( G ) Serum IL-18 level; ( H-J ) Western blot analysis and quantitative of the expression of NLRP3 and Cleaved Gasdermin D in the pancreas; ( K & L ) Representative photos and quantitative of F4/80 staining. Data are presented as mean ± SD ( n = 6 per group). Statistical analyses were performed using GraphPad Prism 8.0. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test. * P < 0.05 indicates a statistically significant difference. CP, chronic pancreatitis; CIRP, Cold-inducible RNA-binding protein; KO, knockout; L-arg, L-arginine; NLRP3, Nod-like receptor family protein 3; TLR4, Toll-like receptor 4; IL-18, interleukin 18; IL-1β, interleukin 1β; Cer, Cerulein

Article Snippet: In the other groups of experimental CP, normal saline (vehicle) or 5, 20 or 100 mg/kg NLRP3 inhibitor [ ] (S3680, Selleck, Inc. CN) was administered by intraperitoneal injection 2 h after the last injection of L-arginine or cerulein.

Techniques: Injection, Western Blot, Expressing, Staining, RNA Binding Assay, Knock-Out

Journal: Inflammation

Article Title: Targeting Cold-Inducible RNA-Binding Protein Attenuates Pancreatic Fibrosis by Suppressing Pyroptosis in Chronic Pancreatitis

doi: 10.1007/s10753-026-02490-x

Figure Lengend Snippet: NLRP3 inhibitor alleviates inflammation and pancreatic fibrosis in Experimental CP. Cerulein-CP was induced by 6 IP injections of cerulein (50 µg/kg/body weight) twice a week for 10 weeks. L-arginine-CP was induced by 2 hourly IP injections of 4.0 g/kg L-arginine twice a week for 10 weeks. At 2 h after the last injection of L-arginine or cerulein each time, normal saline (vehicle) or 5, 20, 100 mg/kg NLRP3 inhibitor was administered by intraperitoneal injection. The animals were sacrificed 10 weeks after the first injection of L-arginine or cerulein. Blood and tissue samples were collected. ( A ) Western blot analysis of the expression of CIRP, NLRP3, Cleaved Caspase-1 and Cleaved Gasdermin D in the pancreas; ( B ) Serum IL-1β level; ( C ) Serum IL-6 level; ( D-G ) Representative photos and quantitative of H&E, Sirius red and F4/80 staining; ( H ) Serum HMGB1 level. Data are presented as mean ± SD ( n = 6 per group). Statistical analyses were performed using GraphPad Prism 8.0. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test. * P < 0.05 indicates a statistically significant difference. CP, chronic pancreatitis; CIRP, Cold-inducible RNA-binding protein; NLRP3, Nod-like receptor family protein 3; IL-6, interleukin 6; IL-1β, interleukin 1β; HMGB1, high mobility group protein B1; Cer, Cerulein; L-arg, L-arginine

Article Snippet: In the other groups of experimental CP, normal saline (vehicle) or 5, 20 or 100 mg/kg NLRP3 inhibitor [ ] (S3680, Selleck, Inc. CN) was administered by intraperitoneal injection 2 h after the last injection of L-arginine or cerulein.

Techniques: Injection, Saline, Western Blot, Expressing, Staining, RNA Binding Assay

Journal: Inflammation

Article Title: Targeting Cold-Inducible RNA-Binding Protein Attenuates Pancreatic Fibrosis by Suppressing Pyroptosis in Chronic Pancreatitis

doi: 10.1007/s10753-026-02490-x

Figure Lengend Snippet: Gasdermin D inhibitor alleviates inflammation and pancreatic fibrosis in Experimental CP. Cerulein-CP was induced by 6 IP injections of cerulein (50 µg/kg/body weight) twice a week for 10 weeks. L-arginine-CP was induced by 2 hourly IP injections of 4.0 g/kg L-arginine twice a week for 10 weeks. At 2 h after the last injection of L-arginine or cerulein each time, normal saline (vehicle) or 5, 20, 50 mg/kg Disulfiram, a potent gasdermin D inhibitor, was administered by intraperitoneal injection. The animals were sacrificed 10 weeks after the first injection of L-arginine or cerulein. Blood and tissue samples were collected. ( A-D ) Representative photos and quantitative of H&E, Sirius red and F4/80 staining; ( E ) Western blot analysis of the expression of CIRP, NLRP3 and Cleaved Gasdermin D in the pancreas; ( F ) Serum IL-18 level. Data are presented as mean ± SD ( n = 6 per group). Statistical analyses were performed using GraphPad Prism 8.0. Data were analyzed by one-way ANOVA with Tukey’s post-hoc test. * P < 0.05 indicates a statistically significant difference. CP, chronic pancreatitis; CIRP, Cold-inducible RNA-binding protein; NLRP3, Nod-like receptor family protein 3; IL-18, interleukin 18

Article Snippet: In the other groups of experimental CP, normal saline (vehicle) or 5, 20 or 100 mg/kg NLRP3 inhibitor [ ] (S3680, Selleck, Inc. CN) was administered by intraperitoneal injection 2 h after the last injection of L-arginine or cerulein.

Techniques: Injection, Saline, Staining, Western Blot, Expressing, RNA Binding Assay