hyperion system Search Results


imaging mass cytometry  (fluidigm)
98
fluidigm imaging mass cytometry
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Imaging Mass Cytometry, supplied by fluidigm, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperiontm series microscope  (Bruker Corporation)
95
Bruker Corporation hyperiontm series microscope
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperiontm Series Microscope, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion series  (Bruker Corporation)
95
Bruker Corporation hyperion series
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Series, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion series ft ir spectrometer  (Bruker Corporation)
95
Bruker Corporation hyperion series ft ir spectrometer
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Series Ft Ir Spectrometer, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion tensor 27 ftir spectrometer  (Bruker Corporation)
99
Bruker Corporation hyperion tensor 27 ftir spectrometer
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Hyperion Tensor 27 Ftir Spectrometer, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hyperion tensor 27 ftir spectrometer/product/Bruker Corporation
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ftir-microscope hyperion 3000 coupled to a tensor 27  (Bruker Corporation)
90
Bruker Corporation ftir-microscope hyperion 3000 coupled to a tensor 27
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Ftir Microscope Hyperion 3000 Coupled To A Tensor 27, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thermomechanical analyzer netzsch tma 402 f2  (NETZSCH)
90
NETZSCH thermomechanical analyzer netzsch tma 402 f2
Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass <t>cytometry.</t> Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.
Thermomechanical Analyzer Netzsch Tma 402 F2, supplied by NETZSCH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion 3000 ft-ir  (Bruker Corporation)
90
Bruker Corporation hyperion 3000 ft-ir
(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). <t>(c)</t> <t>FT-IR</t> imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).
Hyperion 3000 Ft Ir, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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optics hyperion 2000 ftir-microscope  (Bruker Corporation)
90
Bruker Corporation optics hyperion 2000 ftir-microscope
(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). <t>(c)</t> <t>FT-IR</t> imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).
Optics Hyperion 2000 Ftir Microscope, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hyperion mr iii enzyme labeled instrument  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
90
Nanjing Jiancheng Bioengineering Research Institute Co Ltd hyperion mr iii enzyme labeled instrument
(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). <t>(c)</t> <t>FT-IR</t> imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).
Hyperion Mr Iii Enzyme Labeled Instrument, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging system hyperion 3000  (Bruker Corporation)
90
Bruker Corporation imaging system hyperion 3000
(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). <t>(c)</t> <t>FT-IR</t> imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).
Imaging System Hyperion 3000, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vertex 80+hyperion2000  (Bruker Corporation)
90
Bruker Corporation vertex 80+hyperion2000
(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). <t>(c)</t> <t>FT-IR</t> imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).
Vertex 80+Hyperion2000, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Journal: Science translational medicine

Article Title: Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation.

doi: 10.1126/scitranslmed.abn2263

Figure Lengend Snippet: Fig. 7. Spatial-seq reinforces the effects of IFN-producing interfollicular KCs on CD16+ DCs and FBs in the superficial dermis. N = 4 biological replicates; data are shown for the most complex sample as defined by the highest number of spots after quality control steps. (A) Hematoxylin and eosin staining of DLE tissue section cor- responding to spatial-seq data below. Scale bars, 200 m (A to F). (B) Spatial scatter pie plot showing cell type composition based on detection of scRNA-seq signatures corre- sponding to seven cell types. Each spot is represented as a pie chart showing relative cell type proportions. Spot coordinates correspond to tissue location. (C) Spatial heatmap of the IFN FB subset gene signature. Color, scaled expression of each subset gene signature. Only spots meeting an FB prediction score threshold of 0.25 are shown. (D) Spatial heatmap of the LC subset gene signature. All spots are shown. (E) Spatial heatmap of the pDC subset gene signature. All spots are shown. (F) Spatial heatmap of the CD16+ DC subset gene signature. All spots are shown. (G) Representative image of a DLE skin section showing the localization of CD16+ DCs (as indicated by the CD14+CD11c+CD16+ immunophenotype) generated by imaging mass cytometry. Scale bar, 100 m. Insets, subepidermal enrichment of CD16+ DCs. (H) Heatmap depicting the number of the indicated cell types (columns) located within 4 m of each of the CD16+ DCs (rows) located across six DLE (N = 16 CD16+ DCs) and two sub- acute CLE (SCLE; N = 5 CD16+ DCs) sections. Color scale, number of neighboring cells.

Article Snippet: Imaging mass cytometry of tissue sections Formalin-fixed, paraffin-embedded skin biopsy tissue sections from lesional skin of patients with SCLE or DLE were analyzed using a Hyperion imaging cytometry by time of flight system (CyTOF) (Fluidigm) as previously described (34) with modifications of the antibody panel.

Techniques: Control, Staining, Expressing, Generated, Imaging, Mass Cytometry

(a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). (c) FT-IR imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).

Journal: Beilstein Journal of Nanotechnology

Article Title: Site-selective growth of surface-anchored metal-organic frameworks on self-assembled monolayer patterns prepared by AFM nanografting

doi: 10.3762/bjnano.4.71

Figure Lengend Snippet: (a) AFM-topography image of HKUST-1 structures, prepared by spray coating after grafting squared areas of MPA into the DT matrix thiol. (b) Cross section along the white line of (a). (c) FT-IR imaging of the wavenumber region between 1510 cm −1 and 1780 cm −1 . (d) FT-IR spectra representing the area inside the squares (red line in (d) corresponding to red dot in (c)) and outside of the structures (blue line in (d), according to the blue dot in (c)).

Article Snippet: FT-IR-imaging was performed on a Bruker Hyperion 3000 FT-IR (Bruker Optics, Ettlingen, Germany) imaging system equipped with a 20× ATR objective with a Ge-Crystal tip.

Techniques: Imaging