Journal: CNS Neuroscience & Therapeutics

Article Title: Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR 2 / SRC pathway in vascular cognitive impairment mice

doi: 10.1111/cns.14666

Figure Lengend Snippet: Hyperforin ameliorates cognitive impairment in vascular cognitive impairment (VCI) mice as demonstrated by the Y‐maze and Morris water maze (MWM) experiment. (A) The track plot of the spontaneous alternation experiment in the Y‐maze test. (B) The Alternations ratio in Y‐maze test. (C) The track plot of the new arm exploration experiment in the Y‐maze test. (D) The novelty index in Y‐maze test. (E) The escape latency period of the MWM test. (F) The swimming speeds of the mice. (G) The duration of stay in the MWM target quadrant on day 5. (H) The track plot of the MWM test on day 5. (I) The crossing times of the platform in the MWM test. The data are depicted as mean ± SEM ( n = 10–12). * p < 0.05, ** p < 0.01, and *** p < 0.001 versus Sham + NS group; # p < 0.05, ## p < 0.01 versus VCI + NS group.

Article Snippet: The hyperforin (Item No. 19572, purity ≥95%) and the VEGFR 2 inhibitor SU 5416 (S8442, purity ≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Sigma‐Aldrich (St. Louis, MO, USA), correspondingly.

Techniques:

Journal: CNS Neuroscience & Therapeutics

Article Title: Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR 2 / SRC pathway in vascular cognitive impairment mice

doi: 10.1111/cns.14666

Figure Lengend Snippet: Hyperforin reversed the white matter lesions in vascular cognitive impairment (VCI) mice. (A, B) Images of MBP immunofluorescence in the CA3 region of the hippocampus and Luxol fast blue (LFB) staining in the corpus callosum. (C, D) Analysis of myelination quantitatively measured by IOD of LFB in the corpus callosum and IntDen of MBP immunofluorescence in hippocampal CA3 area. (E, F) Immunoreactive bands and quantitative analysis of WB of MBP and β‐Actin. Data are depicted as mean ± SEM ( n = 5 or 3). * p < 0.05, ** p < 0.01 versus Sham + NS group; # p < 0.05, ## p < 0.05 versus VCI + NS group.

Article Snippet: The hyperforin (Item No. 19572, purity ≥95%) and the VEGFR 2 inhibitor SU 5416 (S8442, purity ≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Sigma‐Aldrich (St. Louis, MO, USA), correspondingly.

Techniques: Immunofluorescence, Staining

Journal: CNS Neuroscience & Therapeutics

Article Title: Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR 2 / SRC pathway in vascular cognitive impairment mice

doi: 10.1111/cns.14666

Figure Lengend Snippet: Hyperforin inhibited microglial activation and neuroinflammation in vascular cognitive impairment (VCI) mice. (A) Iba‐1 + immunofluorescence in the hippocampus CA3 region as seen in representative images. (B) Quantification analysis of Iba‐1 + cells in hippocampal CA3 area. (C–F) ELISA analysis of hippocampal contents of IL‐1β, IL‐6, TNF‐α, and IL‐10. Mean ± SEM is used to express data ( n = 5–6 or n = 5, respectively). * p < 0.05, and *** p < 0.001 versus Sham + NS group; # p < 0.05, and ### p < 0.001 versus VCI + NS group.

Article Snippet: The hyperforin (Item No. 19572, purity ≥95%) and the VEGFR 2 inhibitor SU 5416 (S8442, purity ≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Sigma‐Aldrich (St. Louis, MO, USA), correspondingly.

Techniques: Activation Assay, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: CNS Neuroscience & Therapeutics

Article Title: Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR 2 / SRC pathway in vascular cognitive impairment mice

doi: 10.1111/cns.14666

Figure Lengend Snippet: Hyperforin attenuated microglial M1 polarization by regulating VEGFR 2 /SRC pathway in OGD/R‐induced BV2 cells. (A–D) Immunoreactive bands and quantitative analysis of western blot of VRGFR 2 , p‐SRC, SRC, VEGFA, and β‐Actin. (E–H) Immunoreactive bands and quantitative analysis of western blot of CD86, iNOS, ARG1, and β‐Actin. (I–L) Expression levels of IL‐10, TNF‐α, IL‐6, and IL‐1β in the supernatants of BV2 cells. Mean ± SEM is used to represent data ( n = 4–5). ** p < 0.01, and *** p < 0.001 versus control group; # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OGD/R group.

Article Snippet: The hyperforin (Item No. 19572, purity ≥95%) and the VEGFR 2 inhibitor SU 5416 (S8442, purity ≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Sigma‐Aldrich (St. Louis, MO, USA), correspondingly.

Techniques: Western Blot, Expressing, Control