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Image Search Results
Journal: bioRxiv
Article Title: Endogenous formaldehyde scavenges cellular glutathione resulting in cytotoxic redox disruption
doi: 10.1101/2020.05.14.090738
Figure Lengend Snippet: a. Resazurin-based viability assay expressed as % of fluorescence relative to untreated cells in Wild Type (WT), Δ ADH5 , Δ P53 and Δ P53 Δ ADH5 cells exposed to increasing concentrations of formaldehyde (FA) (mean ± s.e.m., n=8). b. Colony survival assay prepared seeding 600 cells in 6-well plates in presence of the indicated concentration of formaldehyde (FA). Colonies were stained and quantified after 10 days (mean ± s.e.m., n=4, one-way ANOVA using Tukey multiple comparison test). c. Western blot showing the induction of p-p53 and g-H2AX after 48 h of exposure to the indicated concentrations of FA or 24 h of exposure to the genotoxic compounds cisplatin (4 μM), hydroxyurea (HU, 1 mM) and mitomycin C (MMC, 1.5 μM). d. Quantitation of p-p53 and g-H2AX western blots using ImageJ (p-p53: FA 0, 60, 150 and cisplatin n=5; HU n=4, MMC n=3. g-H2AX: FA 0, 60, 150, cisplatin and HU n=4; MMC n=3; mean ± s.e.m., unpaired t-test comparing against the same cell line untreated) e. Quantitation of metaphase scoring (mean ± s.e.m., n=49, one-way ANOVA, Tuckey’s multiple comparison test) denoting the induction of chromosome damage by MMC (1.5 μM) but not by FA (150 μM). f. Representative images of Wild Type and Δ ADH5 cells exposed to 150 μM FA and to 1.5 μM MMC (scale bar 1 μm).
Article Snippet: DNA damaging agents were MMC, cisplatin (Santa Cruz, #sc-200896) and
Techniques: Viability Assay, Fluorescence, Clonogenic Cell Survival Assay, Concentration Assay, Staining, Comparison, Western Blot, Quantitation Assay
Journal: Nucleic Acids Research
Article Title: Spatial regulation of DNA damage tolerance protein Rad5 interconnects genome stability maintenance and proteostasis networks
doi: 10.1093/nar/gkad1176
Figure Lengend Snippet: Main reagents and resources used in this work
Article Snippet:
Techniques: Plasmid Preparation, Sequencing, Software