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Image Search Results
Journal: JCI Insight
Article Title: Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen secretion
doi: 10.1172/jci.insight.169213
Figure Lengend Snippet: ( A ) Illustration of treatments and experiments conducted. ( B and C ) H&E-stained sections and quantification (mean ± SEM) of explant skin wounds at different days after wounding as indicated after transdermal treatment with the specific CyD inhibitor NIM811. Arrows indicate wound size by marking epidermal edges. n =3. Magnification, ×4. ( D and E ) Scratch wound assay of keratinocytes and human primary fibroblasts treated with NIM811. ( F ) qPCR analysis (mean ± SEM) of PPIF (CyD) and COL1A1 (collagen 1) expression in explants after transdermal treatment with NIM811 for 7 days. ( G ) Collagen level (mean ± SEM) by hydroxyproline assay after different days of NIM811 treatment. n = 3 for all experiments. *** P < 0.001; * P < 0.05.
Article Snippet: For assessing the collagen deposition in healthy and NIM811-treated human skin samples, we used
Techniques: Staining, Scratch Wound Assay Assay, Expressing, Hydroxyproline Assay