Journal: Frontiers in Immunology

Article Title: The phytohormone abscisic acid enhances remyelination in mouse models of multiple sclerosis

doi: 10.3389/fimmu.2024.1500697

Figure Lengend Snippet: ABA promotes lipid droplet load in macrophages in vitro. (A) Schematic representation showing the experimental set-up: mouse BMDMs were left untreated or treated with 100µg/ml myelin for 24 hours (Mye 24h ) or 72 hours (Mye 72h ). Myelin exposure was performed in the presence or absence of ABA. Created with biorender.com. (B) Representative images of ORO staining of BMDMs exposed to vehicle (PBS) or ABA and treated with myelin for 24 or 72 hours. Scale bars, 50 µm. (C) Quantification of lipid load (defined as percent ORO+ area of total cell area)(n = 3). (D) Mean fluorescence intensity of BODIPY in BMDMs (n = 5) exposed to vehicle or ABA and treated with myelin for 24 or 72 hours, as measured by flow cytometry. Data is represented as relative lipid droplet load compared to vehicle. (E) Internalization of pHrodo™-labelled myelin by BMDMs exposed to vehicle or ABA for 24 hours. Data are measured by flow cytometry and depicted relative to the vehicle treated group. (F) Quantification of total cholesterol (TC), free cholesterol (FC), and esterified cholesterol (EC) in BMDMs (n = 6) exposed to vehicle or ABA and treated with myelin for 0, 24 or 72 hours. (G-I) mRNA expression of Igf1, Tgfβ and Tnf α in BMDMs (n = 4). Each dot represents one well. Data are represented as mean ± SEM and statistically analyzed using a one-way ANOVA with correction for multiple testing or Student’s t-test. *p<0.05, **p<0.01.

Article Snippet: Chemokines and cytokines were measured in plasma using a Bio-Plex assay for tumor necrosis factor α (TNFα) (12002444; Bio-Rad), interferon γ (IFNγ) (12002438; Bio-Rad), interleukin 6 (IL6) (12002241; Bio-Rad), interleukin 10 (IL10) (12002242; Bio-Rad), C-C motif chemokine 5 (CCL5) (12002256; Bio-Rad) and monocyte chemoattractant protein 1 (MCP1) (12002441; Bio-Rad) according to the manufacturer’s instructions.

Techniques: In Vitro, Staining, Fluorescence, Flow Cytometry, Expressing

Journal: Materials

Article Title: Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

doi: 10.3390/ma16144950

Figure Lengend Snippet: SEM images of surfaces of DO and RD PCL/HA scaffolds with 5 wt.% ( a , b ), 30 wt.% ( c , d ) and 50 wt.% ( e , f ) HA. Inset in ( f ) shows details of a hydroxyapatite particle.

Article Snippet: Three different powder mixtures obtained by combining polycaprolactone (PCL, Eurocoating S.p.a., Pergine Valsugana, Italy) and hydroxyapatite (HA, Boc Sciences, Inc., Shirley, NY, USA) in 5, 30 and 50 wt.% were used for scaffold manufacturing by laser powder bed fusion (LPBF) technology, using a Formiga P110 Velocis (EOS GmbH, Munich, Germany) manufacturing system.

Techniques:

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: ( A ) Automatic segmentation with thresholding procedure to select the voxels with a grey level above 250 HU (in blue) corresponding to undiluted CaHA. ( B ) Automatic segmentation with thresholding procedure to select the voxels with a grey level between 0 and 250 HU (in white) corresponding to STS and degraded CaHA; the voxels with a grey level above 250 HU (in pink) correspond to undegraded CaHA. ( C ) 3D model of nodules of undegraded CaHA (in blue) presented in 3D view on Day 0 + 30 min.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques:

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Representative electron microscopy images of CaHA microspheres from in vitro experiment and their surface appearance after incubation with different chemical agents. Scoring increased from 0 (no changes of the surface structure), 1 (slight changes of the surface structure), 2 (intermediate changes of the surface structure) to 3 (extensive changes of the surface structure). A high scoring number indicates significant changes in surface appearance of CaHA microspheres in comparison to smooth, round, and untreated microspheres.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Electron Microscopy, In Vitro, Incubation

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Scoring of surface structure appearance for the incubation of CaHA microspheres in a glass tube for 3 days ( A ) and for 7 days ( B ) with different compounds is shown. N=4 per group. ( C ) Scoring after 3 days of incubation in a human skin equivalent (Phenion) is shown. N=4 per group. ( D ) Incubation of CaHA microspheres in a human ex vivo skin (NativeSkin; n=1) is shown and the respective scoring. *p<0.05 Holm-Sidak method.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Incubation, Ex Vivo

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: CaHA volume as estimated using 3D camera for experimental groups 7 days after injection. N=10 per group. Results are shown as mean±SEM. *p<0.05 vs CaHA ® alone, # p<0.05 vs CaHA+Saline, Dunnett’s test.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: CaHA volume as estimated using µCT for experimental groups 7 days after injection. N=10 per group. Results are shown as mean±SEM. *p<0.05 Dunnett’s test vs CaHA alone.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: 3D models of CaHA injected in pig on Day 0+30min, Day 0+4h, Days 1 and 7. Pure CaHA nodules without STS are in blue, pure CaHA nodules with STS are in pink and diluted CaHA + STS in white/grey.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Effect of STS on CaHA volume after SC injection. Data for CaHA injected with STS are expressed as Total (0-infinity HU); High HU (250-infinity HU) and low HU (0–250 HU). Data are shown as mean±SEM. Statistical comparison was only performed for CaHA vs CaHA+STS high with the only statistically significant difference seen at 90 min. *p<0.05 Student’s t -test.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Effect of STS on CaHA mean density after SC injection. Data for CaHA injected with STS are expressed as Total (0-infinity HU); High HU (250-infinity HU) and low HU (0–250 HU). Data are shown as mean±SEM (N=5 per group). Statistical comparison was performed only for CaHA vs CaHA+STS high and the only statistically significant difference was observed at 120 min.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Effect of STS on CaHA amount (defined as mean density × volume) after SC injection. Data for CaHA injected with STS are expressed as Total (0-infinity HU); High HU (250-infinity HU) and low HU (0–250 HU). Data are shown as mean±SEM. Statistical comparison was performed only for CaHA vs CaHA+STS high and the only statistically significant difference was observed at 7 days. *P<0.05 Student’s t- test.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Frequency plot, density (HU) is presented on x-axis and the number of voxels on y-axis. Plot ( A and B ) show whole HU range (0-infinity) for CaHA and CaHA+STS respectively. Plots ( C and D ) show a section from ( A and B ) respectively but limited to 250-infinity HU range to allow better insight. Data are mean of 5 sites.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques:

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Semi-Quantitative Scoring of Histological Slices. Data Were Analyzed Using Dunnett’s Test. *P <0.05 vs CaHA, #P <0.05 vs CaHA+ Saline

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques:

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Representative histology slices stained with safranin, hematoxylin and eosin for site treated with (from left to right) CaHA only, CaHA+STS and CaHA+saline with low (upper raw) and high (lower raw) magnification.

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Staining

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Summary of Histomorphometry Evaluation of the Injected Area. Results are Shown as mean±SD. *P <0.05 vs CaHA Alone; #P <0.05 vs CaHA+Saline, Dunnett’s Test

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Injection

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: Can Sodium Thiosulfate Act as a Reversal Agent for Calcium Hydroxylapatite Filler? Results of a Preclinical Study

doi: 10.2147/CCID.S271760

Figure Lengend Snippet: Summary of Analysis of Electron Microscopy Images

Article Snippet: Calcium hydroxylapatite (CaHA; Radiesse ® , Merz North America, Inc., Raleigh, NC) injectable filler remains an effective and popular non-HA filler since its introduction a decade ago.

Techniques: Electron Microscopy