hy-p1944 Search Results


apelin 13  (MedChemExpress)
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MedChemExpress apelin 13
<t>Apelin-13</t> <t>pretreatment</t> inhibits SD/H-induced mitochondrial fragmentation and apoptosis of MSCs. (a) CCK-8 assay showed the cell viability of MSCs with or without Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) pretreatment for 24 hours under normoxic or SD/H conditions. (b) CCK-8 assay showed the cell viability of MSCs with or without 0.1 nM Apelin-13 pretreatment for 6, 12, 24, or 36 hours under normoxic or SD/H conditions. (c) Representative images of MitoTracker staining and quantitative analysis of the mitochondrial number and mean mitochondrial area in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (d) Western blotting and quantitative analysis of the protein level of Mfn1/2 and p-Drp1 ser616 in MSCs and Apelin-13-pretreated MSCs under normoxia or SD/H challenge. (e) Representative images of Mito-Sox staining and quantitative analysis of ROS generation in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (f) Representative images of Annexin V/PI staining and quantitative analysis of the apoptotic rate in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.
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Apelin-13 pretreatment inhibits SD/H-induced mitochondrial fragmentation and apoptosis of MSCs. (a) CCK-8 assay showed the cell viability of MSCs with or without Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) pretreatment for 24 hours under normoxic or SD/H conditions. (b) CCK-8 assay showed the cell viability of MSCs with or without 0.1 nM Apelin-13 pretreatment for 6, 12, 24, or 36 hours under normoxic or SD/H conditions. (c) Representative images of MitoTracker staining and quantitative analysis of the mitochondrial number and mean mitochondrial area in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (d) Western blotting and quantitative analysis of the protein level of Mfn1/2 and p-Drp1 ser616 in MSCs and Apelin-13-pretreated MSCs under normoxia or SD/H challenge. (e) Representative images of Mito-Sox staining and quantitative analysis of ROS generation in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (f) Representative images of Annexin V/PI staining and quantitative analysis of the apoptotic rate in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Apelin-13 pretreatment inhibits SD/H-induced mitochondrial fragmentation and apoptosis of MSCs. (a) CCK-8 assay showed the cell viability of MSCs with or without Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) pretreatment for 24 hours under normoxic or SD/H conditions. (b) CCK-8 assay showed the cell viability of MSCs with or without 0.1 nM Apelin-13 pretreatment for 6, 12, 24, or 36 hours under normoxic or SD/H conditions. (c) Representative images of MitoTracker staining and quantitative analysis of the mitochondrial number and mean mitochondrial area in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (d) Western blotting and quantitative analysis of the protein level of Mfn1/2 and p-Drp1 ser616 in MSCs and Apelin-13-pretreated MSCs under normoxia or SD/H challenge. (e) Representative images of Mito-Sox staining and quantitative analysis of ROS generation in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (f) Representative images of Annexin V/PI staining and quantitative analysis of the apoptotic rate in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: CCK-8 Assay, Staining, Western Blot

Apelin-13 pretreatment inhibits SD/H-induced mitochondrial fragmentation in MSCs via regulation of the ERK signaling pathway. (a) Western blotting and quantitative analysis for p-ERK and t-ERK protein level in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (b) Western blotting and quantitative analysis for p-ERK, t-ERK, p-Drp1 ser616, and Drp1 protein level in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (c) Representative images of MitoTracker staining and quantitative analysis of the number of mitochondria and mean mitochondrial area in MSCs, Apelin-13-pretreated MSCs or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (d) Quantitative analysis of ROS generation in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (e) Quantitative analysis of the apoptotic rate of MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (f) Western blotting and quantitative analysis for APJ, p-ERK, and t-ERK protein level in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with ML221 under normoxic or SD/H conditions. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Apelin-13 pretreatment inhibits SD/H-induced mitochondrial fragmentation in MSCs via regulation of the ERK signaling pathway. (a) Western blotting and quantitative analysis for p-ERK and t-ERK protein level in MSCs and Apelin-13-pretreated MSCs under normoxic or SD/H conditions. (b) Western blotting and quantitative analysis for p-ERK, t-ERK, p-Drp1 ser616, and Drp1 protein level in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (c) Representative images of MitoTracker staining and quantitative analysis of the number of mitochondria and mean mitochondrial area in MSCs, Apelin-13-pretreated MSCs or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (d) Quantitative analysis of ROS generation in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (e) Quantitative analysis of the apoptotic rate of MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with U0126 under SD/H condition. (f) Western blotting and quantitative analysis for APJ, p-ERK, and t-ERK protein level in MSCs, Apelin-13-pretreated MSCs, or Apelin-13-pretreated MSCs with ML221 under normoxic or SD/H conditions. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: Western Blot, Staining

Apelin-13 pretreatment improves the angiogenic capacity of MSCs. (a) Representative light images of HUVEC tube formation assay in MSC-CdM, Apelin-13-MSC-CdM, and Apelin-13+U0126-MSC-CdM treatment. (b) Quantitative analysis of the HUVEC tube length in MSC-CdM, Apelin-13-MSC-CdM, and Apelin-13+U0126-MSC-CdM treatment. (c) Expression of proangiogenesis cytokines determined by qRT-PCR. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Apelin-13 pretreatment improves the angiogenic capacity of MSCs. (a) Representative light images of HUVEC tube formation assay in MSC-CdM, Apelin-13-MSC-CdM, and Apelin-13+U0126-MSC-CdM treatment. (b) Quantitative analysis of the HUVEC tube length in MSC-CdM, Apelin-13-MSC-CdM, and Apelin-13+U0126-MSC-CdM treatment. (c) Expression of proangiogenesis cytokines determined by qRT-PCR. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: HUVEC Tube Formation Assay, Expressing, Quantitative RT-PCR

Transplantation of Apelin-13-pretreated MSCs improves heart function following infarction in mice. (a) Representative images of echocardiographic images captured at 4 weeks in mice among the different groups. (b) Quantitative analysis of LVEF and LVFS at 4 weeks in mice from the different groups. (c) Representative images of Masson's trichrome staining of heart sections at 4 weeks in mice among the different groups. (d) Quantitative measurement of heart fibrosis at 4 weeks in mice among the different groups. n = 6 mice for each group. Data are expressed as mean ± SD. Scale bar = 2 mm. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Transplantation of Apelin-13-pretreated MSCs improves heart function following infarction in mice. (a) Representative images of echocardiographic images captured at 4 weeks in mice among the different groups. (b) Quantitative analysis of LVEF and LVFS at 4 weeks in mice from the different groups. (c) Representative images of Masson's trichrome staining of heart sections at 4 weeks in mice among the different groups. (d) Quantitative measurement of heart fibrosis at 4 weeks in mice among the different groups. n = 6 mice for each group. Data are expressed as mean ± SD. Scale bar = 2 mm. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: Transplantation Assay, Staining

Apelin-13 pretreatment improves MSC survival in mice hearts following infarction. (a) Representative images of human mitochondria staining in the ischemic heart of mice at 7 days and 4 weeks among the different groups. (b) Quantitative analysis of human mitochondria positive cells in ischemic mice hearts at 7 days and 4 weeks among the different groups. (c) Alu-sx expression was evaluated by PCR in the sham, MI, MSCs, or Apelin-13-MSCs group. n = 6 mice for each group. Data are expressed as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Apelin-13 pretreatment improves MSC survival in mice hearts following infarction. (a) Representative images of human mitochondria staining in the ischemic heart of mice at 7 days and 4 weeks among the different groups. (b) Quantitative analysis of human mitochondria positive cells in ischemic mice hearts at 7 days and 4 weeks among the different groups. (c) Alu-sx expression was evaluated by PCR in the sham, MI, MSCs, or Apelin-13-MSCs group. n = 6 mice for each group. Data are expressed as mean ± SD. ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: Staining, Expressing

Transplantation of Apelin-13-pretreated MSCs improves angiogenesis in mouse hearts following infarction. (a) Representative images of CD31 staining and α -SMA staining in the ischemic heart of mice at 4 weeks among the different groups. (b) Quantitative analysis of the capillary density in the ischemic heart at 4 weeks among the different groups. (c) Quantitative analysis of the arteriole density in the ischemic heart at 4 weeks among the different groups. n = 6 mice for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Journal: Stem Cells International

Article Title: Apelin-13 Pretreatment Promotes the Cardioprotective Effect of Mesenchymal Stem Cells against Myocardial Infarction by Improving Their Survival

doi: 10.1155/2022/3742678

Figure Lengend Snippet: Transplantation of Apelin-13-pretreated MSCs improves angiogenesis in mouse hearts following infarction. (a) Representative images of CD31 staining and α -SMA staining in the ischemic heart of mice at 4 weeks among the different groups. (b) Quantitative analysis of the capillary density in the ischemic heart at 4 weeks among the different groups. (c) Quantitative analysis of the arteriole density in the ischemic heart at 4 weeks among the different groups. n = 6 mice for each group. Data are expressed as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: For Apelin-13 (HY-P1944A, MCE) pretreatment, MSCs were cultured in fresh complete medium supplemented with different concentrations of Apelin-13 (0.01, 0.05, 0.1, 0.5, and 1 nM) under normoxia (95% air and 5% CO 2 ) for 24 hours and then exposed to serum deprivation and hypoxia (SD/H) for 48 hours.

Techniques: Transplantation Assay, Staining