Journal: Respiratory research

Article Title: MUC1 attenuates neutrophilic airway inflammation in asthma by reducing NLRP3 inflammasome-mediated pyroptosis through the inhibition of the TLR4/MyD88/NF-κB pathway.

doi: 10.1186/s12931-023-02550-y

Figure Lengend Snippet: Fig. 6 TAK-242 partially ameliorates GO-203-exacerbated neutrophilic airway inflammation in OVA/LPS-induced asthmatic mouse. (A, B) HE staining and PAS staining were performed on lung sections in the indicated groups. Images were taken under a microscope at 200× magnification. Scale bar: 50 μm. (C, D) Inflammatory infiltration and hyperplasia of goblet cells were quantified using inflammation and PAS scores. (E) Statistical analysis of the total in flammatory cells, macrophages, neutrophils, eosinophils, and lymphocytes in BALF. (F) The mRNA expression levels of IL-6, IL-8, IL-18, IL-1β, and TNF-α in lung tissues were detected using RT-qPCR. LDH = lactate dehydrogenase. Data are expressed as mean ± SD (n = 6 animals per group) and were analyzed using one-way analysis of variance,*p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001

Article Snippet: Mice in the OVA/LPS + GO-203 and OVA/ LPS + GO-203 + TAK-242 groups were intraperitoneally injected with the MUC1 inhibitor GO-203 (MedChemExpress, NJ, USA) (20 mg/kg) 2 h before each challenge.

Techniques: Staining, Microscopy, Expressing, Quantitative RT-PCR

Journal: Respiratory research

Article Title: MUC1 attenuates neutrophilic airway inflammation in asthma by reducing NLRP3 inflammasome-mediated pyroptosis through the inhibition of the TLR4/MyD88/NF-κB pathway.

doi: 10.1186/s12931-023-02550-y

Figure Lengend Snippet: Fig. 7 TAK-242 downregulates GO-203-caused TLR4/MyD88/NF-κB pathway activation and NLRP3 inflammasome-mediated pyroptosis in OVA/LPS- induced asthmatic mouse. (A) The protein expression levels of MUC1, TLR4, MyD88, p-p65, and p65 in lung tissue were determined via immunoblotting. (B) The expression of MUC1, TLR4, and p-p65 in the bronchi were determined via immunofluorescence staining. Images were taken using a fluorescent microscope at 400× magnification. Red: MUC1; green: TLR4 and p-p65; blue: DAPI; scale bar: 50 μm. (C) The p65 protein levels in the nuclear and cyto plasmic fractions in lung tissue were analyzed via immunoblotting. (D) The protein expression levels of NLRP3, caspase-1, cleaved caspase-1, GSDMD, GSDMD-N, IL-18, and IL-1β in lung tissue were determined via immunoblotting. (E) LDH release into BALF was detected via LDH release assay. (F) The expression of NLRP3 and caspase-1 in the bronchi were determined via immunofluorescence staining. Images were taken using a fluorescent microscope at 400× magnification. Red: NLRP3; green: caspase-1; blue: DAPI; scale bar: 50 μm. LDH = lactate dehydrogenase. Data are expressed as mean ± SD (n = 6 animals per group) and were analyzed using one-way analysis of variance, *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001

Article Snippet: Mice in the OVA/LPS + GO-203 and OVA/ LPS + GO-203 + TAK-242 groups were intraperitoneally injected with the MUC1 inhibitor GO-203 (MedChemExpress, NJ, USA) (20 mg/kg) 2 h before each challenge.

Techniques: Activation Assay, Expressing, Western Blot, Immunofluorescence, Staining, Microscopy, Lactate Dehydrogenase Assay