Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: Type I collagen mediates tumor progression through integrin α2β1. ( A ) Western blotting of integrin α2 and integrin β1 in Saos-2 and Collagen-cultured Saos-2 cells. ( B ) Relative cell proliferation of collagen-cultured Saos-2 cells treated with α2β1 integrin ligand peptide (0.5mM, 48 hours). ( C ) tumor volume of collagen-cultured Saos-2 cells tumor-bearing mice pre-treated with α2β1 integrin ligand peptide (0.5 mM, 48 hours treatment before injection). ( D ) Relative colony formation of collagen pre-cultured Saos-2 cells pre-treated with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours). ( E ) Tumorigenesis of collagen-cultured Saos-2 cells pre-treated with α2β1 integrin ligand peptide (0.5 mM, 48 hours treatment before injection) in NOS-SCID mice. ( F ) Relative transwell cells number of 2D collagen-cultured Sao-2 cells treated with PBS or α2β1 integrin ligand peptide (0.5mM, 48 hours). ( G ) the expression of integrin α2 and integrin β1 in the osteosarcoma tissues from HD and LD patients. ( H ) The correlation analysis of type I collagen expression and integrin α2 expression detected by Western blotting (R =0.5934). ( I ) The correlation analysis of type I collagen expression and integrin β1 expression detected by Western blotting (R =0.7431). *Indicates P <0.05.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Western Blot, Cell Culture, Injection, Expressing

Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: JAK/STAT3 acts as integrin downstream signal involved in tumor regulation. ( A ) the expression of phosphorylated and total JAK1, JAK2, STAT3 in Saos-2, collagen-cultured Saos-2 cells and collagen-cultured Saos-2 cells treated with α2β1 integrin ligand peptide (0.5mM, 48 hours). ( B ) Relative cell proliferation collagen-cultured Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours). ( C ) Tumor volume of Collagen-cultured Saos-2 cell tumor-bearing mice treated with PBS or FLLL32 (1 mg/kg, treated on day 14 and 17 after injection). ( D ) Relative colony formation of Collagen-cultured Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours). ( E ) Tumorigenesis of collagen treated Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours before injection) in NOD-SCID mice. ( F ) Expression of phosphorylated JAK1, JAK2 and STAT3 in tumor tissues from HD and LD patients. *Indicates P <0.05.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Expressing, Cell Culture, Injection

Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: Inhibition of integrin α2β1 enhances the therapeutic effects of chemotherapy/radiotherapy on osteosarcoma. ( A ) Cytotoxicity of PBS, IFO (2 mg/kg, 48 hours), and IFO (2 mg/kg, 48 hours) combined with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) to Saos-2 and MG-63 cells. ( B ) Cytotoxicity of PBS, radiotherapy (0.5Gy) and radiotherapy (0.5Gy) combined with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) to Saos-2 and MG-63 cells. ( C ) The presentative image and tumor volume of Saos-2-bearing mice treated with PBS, IFO (2 mg/kg, 48 hours), α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) and IFO combined with α2β1 integrin ligand peptide. The scale bar is 0.5 cm. ( D ) The survival time of Saos-2-bearing mice treated with PBS, IFO (2 mg/kg, 48 hours), α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) and IFO combined with α2β1 integrin ligand peptide. ( E ) Tumor volumes of Saos-2-bearing mice treated with PBS, α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours), radiotherapy (0.5Gy), radiotherapy combined with α2β1 integrin ligand peptide. ( F ) the survival time of Saos-2-bearing mice treated with PBS, α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours), radiotherapy (0.5Gy), radiotherapy combined with α2β1 integrin ligand peptide. *Indicates P <0.05, **Indicates P <0.01.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Inhibition