hy-p1036 Search Results


compstatin  (MedChemExpress)
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MedChemExpress compstatin
The Role of Complement in JIPh_Ec70 and JIPh_Kp127 Internalisation. (A) Comparison of SYBR‐labelled JIPh_Ec70 and JIPh_Kp127 monocyte internalization over a 4 h time‐course ( n = 6). (B) Ec70 and Kp127 phage internalization following inhibition of complement using <t>compstatin</t> (C3 inhibitor) or heat inactivation. Intracellular phage was measured by flow cytometry (SYBR labeling), plaque‐forming units (viable phage), and qPCR (intracellular genomes) ( n = 8 ). (C) C3 internalization was quantified by flow cytometry in phage+ and phage‐monocytes following complement inactivation using compstatin and heat inactivation ( n = 7 ). (D) Immunofluorescent imaging of monocytes treated with SYBR‐labelled phages (green) ± C3 inhibitor compstatin. Internalized C3 was labelled in red and cell nucleus in blue. Detected phages and C3 marked with a white arrow, scale bar = 2 µm. Data presented with mean ± SEM, a paired t ‐test, (E, F) RM two‐way ANOVA test, * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001. C3, complement factor 3; MFI, median fluorescent intensity; PFU, plaque‐forming unit.
Compstatin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The Role of Complement in JIPh_Ec70 and JIPh_Kp127 Internalisation. (A) Comparison of SYBR‐labelled JIPh_Ec70 and JIPh_Kp127 monocyte internalization over a 4 h time‐course ( n = 6). (B) Ec70 and Kp127 phage internalization following inhibition of complement using compstatin (C3 inhibitor) or heat inactivation. Intracellular phage was measured by flow cytometry (SYBR labeling), plaque‐forming units (viable phage), and qPCR (intracellular genomes) ( n = 8 ). (C) C3 internalization was quantified by flow cytometry in phage+ and phage‐monocytes following complement inactivation using compstatin and heat inactivation ( n = 7 ). (D) Immunofluorescent imaging of monocytes treated with SYBR‐labelled phages (green) ± C3 inhibitor compstatin. Internalized C3 was labelled in red and cell nucleus in blue. Detected phages and C3 marked with a white arrow, scale bar = 2 µm. Data presented with mean ± SEM, a paired t ‐test, (E, F) RM two‐way ANOVA test, * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001. C3, complement factor 3; MFI, median fluorescent intensity; PFU, plaque‐forming unit.

Journal: European Journal of Immunology

Article Title: Differences in Phage Recognition and Immunogenicity Contribute to Divergent Human Immune Responses to Escherichia coli and Klebsiella pneumoniae Phages

doi: 10.1002/eji.202451543

Figure Lengend Snippet: The Role of Complement in JIPh_Ec70 and JIPh_Kp127 Internalisation. (A) Comparison of SYBR‐labelled JIPh_Ec70 and JIPh_Kp127 monocyte internalization over a 4 h time‐course ( n = 6). (B) Ec70 and Kp127 phage internalization following inhibition of complement using compstatin (C3 inhibitor) or heat inactivation. Intracellular phage was measured by flow cytometry (SYBR labeling), plaque‐forming units (viable phage), and qPCR (intracellular genomes) ( n = 8 ). (C) C3 internalization was quantified by flow cytometry in phage+ and phage‐monocytes following complement inactivation using compstatin and heat inactivation ( n = 7 ). (D) Immunofluorescent imaging of monocytes treated with SYBR‐labelled phages (green) ± C3 inhibitor compstatin. Internalized C3 was labelled in red and cell nucleus in blue. Detected phages and C3 marked with a white arrow, scale bar = 2 µm. Data presented with mean ± SEM, a paired t ‐test, (E, F) RM two‐way ANOVA test, * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001. C3, complement factor 3; MFI, median fluorescent intensity; PFU, plaque‐forming unit.

Article Snippet: To investigate the role of complement factor 3 (C3) in phage internalization, monocytes were preincubated with 50 μM compstatin (MedchemExpress, # HY‐P1036) for 15 min in media with serum before treating with phages.

Techniques: Comparison, Inhibition, Flow Cytometry, Labeling, Imaging