hy-p0198 Search Results


neuropeptide y  (MedChemExpress)
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Neuropeptide Y (human,rat,mouse) is involved in Alzheimer's disease (AD) and protects rat cortical neurons against β-Amyloid toxicity.
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raw264 7 cells  (MedChemExpress)
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MedChemExpress raw264 7 cells
Figure 5. Intermittent fasting blocks the DMM-induced increase of the pro-inflammatory, pro-osteoclastic, and pro-neurite outgrowth factor NPY by osteocytes. A,B) Representative immunohistochemical staining images of NPY A) and quantification of the mean staining intensity for NPY B) in the tibial subchondral bones from sham- or DMM-operated 3-month-old young mice receiving ad libitum feeding or intermittent fasting for 2 or 4 weeks. Scale bars: 50 μm. n = 8 per group. C) The concentration of NPY in the culture medium of osteocytes (OCYSham-CM, OCYDMM-CM, and OCYDMM + Fasting- CM) from sham, DMM, and DMM + intermittent fasting mice assessed by ELISA. n = 6 per group. D–G) qRT-PCR of Il-1𝛼D), Il-1𝛽E), Il-6 F), and Tnf-𝛼 G) in monocyte/macrophage cell <t>line</t> <t>RAW264.7</t> with different treatments. n = 3 per group. H) Western blot analysis for IL-1𝛼, IL-1𝛽, IL-6, and TNF-𝛼 in RAW264.7 with different treatments. I,J) Representative TRAP staining images I) and quantification of the number of osteoclasts (>3 nuclei; J). Scale bar: 100 μm. n = 3 per group. K,L) Representative staining images of calcein AM K) and quantification of the neurite lengths L) in the calcein AM-stained live CAD cells. Scale bar: 50 μm. n = 3 per group. Data are presented as mean ± SD. For panel (B): Two-way ANOVA with Bonferroni post hoc test. For panel (C-G and L): Unpaired, two-tailed student’s t-test (differences between control and NPY groups, or control and OCY-CM from different groups) or one-way ANOVA with Bonferroni post hoc test (differences among OCY-CM or MM-CM from different groups). *p < 0.05, **p < 0.01, ***p < 0.001.
Raw264 7 Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Intermittent fasting blocks the DMM-induced increase of the pro-inflammatory, pro-osteoclastic, and pro-neurite outgrowth factor NPY by osteocytes. A,B) Representative immunohistochemical staining images of NPY A) and quantification of the mean staining intensity for NPY B) in the tibial subchondral bones from sham- or DMM-operated 3-month-old young mice receiving ad libitum feeding or intermittent fasting for 2 or 4 weeks. Scale bars: 50 μm. n = 8 per group. C) The concentration of NPY in the culture medium of osteocytes (OCYSham-CM, OCYDMM-CM, and OCYDMM + Fasting- CM) from sham, DMM, and DMM + intermittent fasting mice assessed by ELISA. n = 6 per group. D–G) qRT-PCR of Il-1𝛼D), Il-1𝛽E), Il-6 F), and Tnf-𝛼 G) in monocyte/macrophage cell line RAW264.7 with different treatments. n = 3 per group. H) Western blot analysis for IL-1𝛼, IL-1𝛽, IL-6, and TNF-𝛼 in RAW264.7 with different treatments. I,J) Representative TRAP staining images I) and quantification of the number of osteoclasts (>3 nuclei; J). Scale bar: 100 μm. n = 3 per group. K,L) Representative staining images of calcein AM K) and quantification of the neurite lengths L) in the calcein AM-stained live CAD cells. Scale bar: 50 μm. n = 3 per group. Data are presented as mean ± SD. For panel (B): Two-way ANOVA with Bonferroni post hoc test. For panel (C-G and L): Unpaired, two-tailed student’s t-test (differences between control and NPY groups, or control and OCY-CM from different groups) or one-way ANOVA with Bonferroni post hoc test (differences among OCY-CM or MM-CM from different groups). *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Intermittent Fasting Targets Osteocyte Neuropeptide Y to Relieve Osteoarthritis.

doi: 10.1002/advs.202400196

Figure Lengend Snippet: Figure 5. Intermittent fasting blocks the DMM-induced increase of the pro-inflammatory, pro-osteoclastic, and pro-neurite outgrowth factor NPY by osteocytes. A,B) Representative immunohistochemical staining images of NPY A) and quantification of the mean staining intensity for NPY B) in the tibial subchondral bones from sham- or DMM-operated 3-month-old young mice receiving ad libitum feeding or intermittent fasting for 2 or 4 weeks. Scale bars: 50 μm. n = 8 per group. C) The concentration of NPY in the culture medium of osteocytes (OCYSham-CM, OCYDMM-CM, and OCYDMM + Fasting- CM) from sham, DMM, and DMM + intermittent fasting mice assessed by ELISA. n = 6 per group. D–G) qRT-PCR of Il-1𝛼D), Il-1𝛽E), Il-6 F), and Tnf-𝛼 G) in monocyte/macrophage cell line RAW264.7 with different treatments. n = 3 per group. H) Western blot analysis for IL-1𝛼, IL-1𝛽, IL-6, and TNF-𝛼 in RAW264.7 with different treatments. I,J) Representative TRAP staining images I) and quantification of the number of osteoclasts (>3 nuclei; J). Scale bar: 100 μm. n = 3 per group. K,L) Representative staining images of calcein AM K) and quantification of the neurite lengths L) in the calcein AM-stained live CAD cells. Scale bar: 50 μm. n = 3 per group. Data are presented as mean ± SD. For panel (B): Two-way ANOVA with Bonferroni post hoc test. For panel (C-G and L): Unpaired, two-tailed student’s t-test (differences between control and NPY groups, or control and OCY-CM from different groups) or one-way ANOVA with Bonferroni post hoc test (differences among OCY-CM or MM-CM from different groups). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For MM-CM preparation, the culture of RAW264.7 cells was added with OCYCM from different groups, NPY (0.1 nm; Cat. No. HY-P0198A; MedChemExpress, Monmouth Junction, USA) + un-cultured control medium of osteocytes (NPY group for short), or vehicle of NPY + un-cultured control medium of osteocytes (Control group for short).

Techniques: Immunohistochemical staining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Two Tailed Test, Control