Journal: iScience

Article Title: HOXA11 promotes lymphatic metastasis of gastric cancer via transcriptional activation of TGFβ1

doi: 10.1016/j.isci.2023.107346

Figure Lengend Snippet: HOXA11 regulates EMT through TGFβ signaling to promote gastric cancer cell metastasis (A–D) Gastric cancer cells with HOXA11 overexpression (A and C) or knockdown (B and D) affecting the expression of TGFβ signaling molecules as well as E-cadherin, N-cadherin, Vimentin, Slug and Snail key molecules in EMT were determined by RT-qPCR and western blot assays. (E and F) The expression of E-cadherin, N-cadherin and Vimentin in gastric cancer cells affected by HOXA11 overexpression (E) or knockdown (F) was determined by cellular immunofluorescence assays, Scale bar: 20 μm. (G and H) Protein expression of TGFβ1 and EMT key molecules was determined by treating HOXA11 overexpressed (G) or knocked down (H) gastric cancer cells with the TGFβ1 signaling pathway inhibitor P144. The data are presented as the mean ± SEM of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Disitertide (P144) , MedChemExpress , HY-P0118.

Techniques: Over Expression, Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence

Journal: iScience

Article Title: HOXA11 promotes lymphatic metastasis of gastric cancer via transcriptional activation of TGFβ1

doi: 10.1016/j.isci.2023.107346

Figure Lengend Snippet:

Article Snippet: Disitertide (P144) , MedChemExpress , HY-P0118.

Techniques: Recombinant, Membrane, Chromatin Immunoprecipitation, Magnetic Beads, Luciferase, Reporter Gene Assay, Enzyme-linked Immunosorbent Assay, Software