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lipoteichoic acid  (MedChemExpress)
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MedChemExpress lipoteichoic acid
Galectin-3 exerts antibacterial activity by binding to the teichoic acids. A-C Fourier transform infrared (FTIR) spectrum analysis of peptidoglycan (PGN), <t>lipoteichoic</t> acid (LTA), and wall teichoic acid (WTA). D Lysozyme digestion test of PGN. E Utilization of ELISA to evaluate the binding affinity of galectin-3 to PGN, LTA, and WTA. F Determination of the MIC through microdilution assay to assess the effect of LTA and WTA on the inhibition of S. suis by galectin-3. G Zeta potential determination. H Percent of bacterial cell surface hydrophobicity. I Membrane potential was determined by fluorescence intensity measurements using a membrane potential probe, the DiOC 2 (3) (50 μM). Data are presented as mean ± SD derived from three independent experiments. One-way ANOVA was employed to evaluate statistical significance. NS, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.
Lipoteichoic Acid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Galectin-3 exerts antibacterial activity by binding to the teichoic acids. A-C Fourier transform infrared (FTIR) spectrum analysis of peptidoglycan (PGN), lipoteichoic acid (LTA), and wall teichoic acid (WTA). D Lysozyme digestion test of PGN. E Utilization of ELISA to evaluate the binding affinity of galectin-3 to PGN, LTA, and WTA. F Determination of the MIC through microdilution assay to assess the effect of LTA and WTA on the inhibition of S. suis by galectin-3. G Zeta potential determination. H Percent of bacterial cell surface hydrophobicity. I Membrane potential was determined by fluorescence intensity measurements using a membrane potential probe, the DiOC 2 (3) (50 μM). Data are presented as mean ± SD derived from three independent experiments. One-way ANOVA was employed to evaluate statistical significance. NS, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Veterinary Research

Article Title: Galectin-3: a novel antimicrobial host factor identified in goat nasal mucus

doi: 10.1186/s13567-025-01586-w

Figure Lengend Snippet: Galectin-3 exerts antibacterial activity by binding to the teichoic acids. A-C Fourier transform infrared (FTIR) spectrum analysis of peptidoglycan (PGN), lipoteichoic acid (LTA), and wall teichoic acid (WTA). D Lysozyme digestion test of PGN. E Utilization of ELISA to evaluate the binding affinity of galectin-3 to PGN, LTA, and WTA. F Determination of the MIC through microdilution assay to assess the effect of LTA and WTA on the inhibition of S. suis by galectin-3. G Zeta potential determination. H Percent of bacterial cell surface hydrophobicity. I Membrane potential was determined by fluorescence intensity measurements using a membrane potential probe, the DiOC 2 (3) (50 μM). Data are presented as mean ± SD derived from three independent experiments. One-way ANOVA was employed to evaluate statistical significance. NS, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: His-tag antibody (Proteintech, 1:5000, HRP-66005), galectin-3/LGALS3 antibody (Abclonal, IHC, 1:100; WB, 1:2000; A11198), β-actin antibody (Abways, 1:5000, AB0035), Enhanced ATP Assay Kit (Beyotime, S0027), Bacterial Viability Assay Kit (Beyotime, C2030S), BCA Protein Quantification kit (Vazyme, E112-01), DiOC 2 (3) (Solarbio, 905-96-4), Alkaline phosphatase assay kit (Nanjing Jiancheng, A059-2-2), recombinant human galectin-3/LGALS3 Protein (ABclonal, RP00057), recombinant Sumo-Flag-His protein (Bioworlde, NBP0540-2), lipoteichoic acid from Staphylococcus aureus (MCE, HY-N9481), and peptidoglycan from Staphylococcus aureus (Maclin Reagents, P742432).

Techniques: Activity Assay, Binding Assay, Fourier Transform Infrared Spectroscopy, Enzyme-linked Immunosorbent Assay, Microdilution Assay, Inhibition, Zeta Potential Analyzer, Cell Surface Hydrophobicity, Membrane, Fluorescence, Derivative Assay