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ag490  (MedChemExpress)
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MedChemExpress ag490
Yin Yang 1 promotes epithelial-mesenchymal transition via activation of the Janus kinase 2/signal transducer and activator of transcription 3 pathway. a P < 0.001. A: Total and phosphorylated protein levels of Janus kinase (JAK) 2 and signal transducer and activator of transcription 3 (STAT3) in AGS and SGC7901 cells cocultured with Helicobacter pylori were analyzed by Western blotting; B: Expression of JAK2/STAT3 and epithelial-mesenchymal transition (EMT)-related proteins in AGS and SGC7901 cell lines with Yin Yang 1 (YY1) knockdown and overexpression; C: GEPIA database was used to analyze the relationship between YY1 and JAK2 expression in gastric cancer tissues; D: Joint Array for Public Research algorithm predicted the YY1 binding site in JAK2 promoter; E: Interaction between YY1 and JAK3 was detected using chromatin immunoprecipitation assay; F: Activation of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression following addition of coumermycin A1 in AGS cells with YY1 knockdown, and inhibition of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression with addition of <t>AG490</t> in AGS cells with YY1 overexpression. YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; JAK: Janus kinase; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research; TPM: Transcript per million.
Ag490, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Yin Yang 1 promotes epithelial-mesenchymal transition via activation of the Janus kinase 2/signal transducer and activator of transcription 3 pathway. a P < 0.001. A: Total and phosphorylated protein levels of Janus kinase (JAK) 2 and signal transducer and activator of transcription 3 (STAT3) in AGS and SGC7901 cells cocultured with Helicobacter pylori were analyzed by Western blotting; B: Expression of JAK2/STAT3 and epithelial-mesenchymal transition (EMT)-related proteins in AGS and SGC7901 cell lines with Yin Yang 1 (YY1) knockdown and overexpression; C: GEPIA database was used to analyze the relationship between YY1 and JAK2 expression in gastric cancer tissues; D: Joint Array for Public Research algorithm predicted the YY1 binding site in JAK2 promoter; E: Interaction between YY1 and JAK3 was detected using chromatin immunoprecipitation assay; F: Activation of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression following addition of coumermycin A1 in AGS cells with YY1 knockdown, and inhibition of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression with addition of AG490 in AGS cells with YY1 overexpression. YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; JAK: Janus kinase; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research; TPM: Transcript per million.

Journal: World Journal of Clinical Oncology

Article Title: Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori -induced gastric cancer progression

doi: 10.5306/wjco.v16.i12.112626

Figure Lengend Snippet: Yin Yang 1 promotes epithelial-mesenchymal transition via activation of the Janus kinase 2/signal transducer and activator of transcription 3 pathway. a P < 0.001. A: Total and phosphorylated protein levels of Janus kinase (JAK) 2 and signal transducer and activator of transcription 3 (STAT3) in AGS and SGC7901 cells cocultured with Helicobacter pylori were analyzed by Western blotting; B: Expression of JAK2/STAT3 and epithelial-mesenchymal transition (EMT)-related proteins in AGS and SGC7901 cell lines with Yin Yang 1 (YY1) knockdown and overexpression; C: GEPIA database was used to analyze the relationship between YY1 and JAK2 expression in gastric cancer tissues; D: Joint Array for Public Research algorithm predicted the YY1 binding site in JAK2 promoter; E: Interaction between YY1 and JAK3 was detected using chromatin immunoprecipitation assay; F: Activation of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression following addition of coumermycin A1 in AGS cells with YY1 knockdown, and inhibition of the EMT pathway and phosphorylated Janus kinase 2/phosphorylated signal transducer and activator of transcription 3 expression with addition of AG490 in AGS cells with YY1 overexpression. YY1: Yin Yang 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; JAK: Janus kinase; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research; TPM: Transcript per million.

Article Snippet: Coumermycin A1 [10 μM; HY-12000, MCE (Med Chem Express)] and AG490 (100 nM; HY-N7452, MCE) were added to the medium to activate or inhibit the pathway, respectively.

Techniques: Activation Assay, Western Blot, Expressing, Knockdown, Over Expression, Binding Assay, Chromatin Immunoprecipitation, Inhibition

Subcutaneous tumor formation experiment in nude mice. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. A: Representative images of subcutaneous tumors; B and C: Tumor volume growth curve and tumor weight measurements; D: Hematoxylin and eosin staining of subcutaneous tumors from four groups; E: Immunohistochemistry of subcutaneous tumors from four groups (Ki-67); F: Protein expression of the epithelial-mesenchymal transition and Janus kinase 2/signal transducer and activator of transcription 3 pathway with addition of AG490 from subcutaneous tumors. YY1: Yin Yang 1; JAK2: Janus kinase 2; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research.

Journal: World Journal of Clinical Oncology

Article Title: Yin Yang 1 activates JAK-STAT3-mediated epithelial-mesenchymal transition in Helicobacter pylori -induced gastric cancer progression

doi: 10.5306/wjco.v16.i12.112626

Figure Lengend Snippet: Subcutaneous tumor formation experiment in nude mice. a P < 0.05, b P < 0.01, c P < 0.001, d P < 0.0001. A: Representative images of subcutaneous tumors; B and C: Tumor volume growth curve and tumor weight measurements; D: Hematoxylin and eosin staining of subcutaneous tumors from four groups; E: Immunohistochemistry of subcutaneous tumors from four groups (Ki-67); F: Protein expression of the epithelial-mesenchymal transition and Janus kinase 2/signal transducer and activator of transcription 3 pathway with addition of AG490 from subcutaneous tumors. YY1: Yin Yang 1; JAK2: Janus kinase 2; STAT3: Signal transducer and activator of transcription 3; pJAK2: Phosphorylated Janus kinase 2; pSTAT3: Phosphorylated signal transducer and activator of transcription 3; JASPAR: Joint Array for Public Research.

Article Snippet: Coumermycin A1 [10 μM; HY-12000, MCE (Med Chem Express)] and AG490 (100 nM; HY-N7452, MCE) were added to the medium to activate or inhibit the pathway, respectively.

Techniques: Staining, Immunohistochemistry, Expressing