hy-n6939 Search Results


pab  (MedChemExpress)
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<t>PAB</t> inhibited the proliferation and viability in canine mammary tumor U27 cells. (A) Chemical structure of PAB. (B,C) Canine mammary tumor U27 cells were exposed to different concentrations of PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) for 24 h, LDH activity (B) in the supernatant was quantified using a colorimetric assay by measuring absorbance at 490 nm and the cell viability (C) of canine mammary tumor U27 cells was detected by CCK-8 assay. (D) Representative images of the proliferative ability of canine mammary tumor U27 cells treated with PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) via crystal violet cell proliferation assay. (E) Quantitative analysis of cells stained with crystal violet. Values represent mean ± SD of three biological replicates. (F) Representative images of morphological changes of U27 cells after PAB exposure at varying time <t>points.</t> <t>DMSO-treated</t> control cells maintained normal morphology and PAB-treated (2.5 μM) cells exhibited significant rounding. Scale bar: 200 μm. Unpaired two-tailed Student’s t -test or one-way ANOVA was performed. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.
Pab, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PAB inhibited the proliferation and viability in canine mammary tumor U27 cells. (A) Chemical structure of PAB. (B,C) Canine mammary tumor U27 cells were exposed to different concentrations of PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) for 24 h, LDH activity (B) in the supernatant was quantified using a colorimetric assay by measuring absorbance at 490 nm and the cell viability (C) of canine mammary tumor U27 cells was detected by CCK-8 assay. (D) Representative images of the proliferative ability of canine mammary tumor U27 cells treated with PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) via crystal violet cell proliferation assay. (E) Quantitative analysis of cells stained with crystal violet. Values represent mean ± SD of three biological replicates. (F) Representative images of morphological changes of U27 cells after PAB exposure at varying time points. DMSO-treated control cells maintained normal morphology and PAB-treated (2.5 μM) cells exhibited significant rounding. Scale bar: 200 μm. Unpaired two-tailed Student’s t -test or one-way ANOVA was performed. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.

Journal: Frontiers in Veterinary Science

Article Title: Pseudolaric acid B induces G2/M phase arrest in canine mammary tumor cells by targeting CDK1

doi: 10.3389/fvets.2025.1644200

Figure Lengend Snippet: PAB inhibited the proliferation and viability in canine mammary tumor U27 cells. (A) Chemical structure of PAB. (B,C) Canine mammary tumor U27 cells were exposed to different concentrations of PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) for 24 h, LDH activity (B) in the supernatant was quantified using a colorimetric assay by measuring absorbance at 490 nm and the cell viability (C) of canine mammary tumor U27 cells was detected by CCK-8 assay. (D) Representative images of the proliferative ability of canine mammary tumor U27 cells treated with PAB (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) via crystal violet cell proliferation assay. (E) Quantitative analysis of cells stained with crystal violet. Values represent mean ± SD of three biological replicates. (F) Representative images of morphological changes of U27 cells after PAB exposure at varying time points. DMSO-treated control cells maintained normal morphology and PAB-treated (2.5 μM) cells exhibited significant rounding. Scale bar: 200 μm. Unpaired two-tailed Student’s t -test or one-way ANOVA was performed. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.

Article Snippet: Pseudolaric acid B, PAB (10 mM in DMSO) (#HY-N6939, MedChemExpress, USA) and Ro-3306 (10 mM) (#HY-12529, MCE, Shanghai, China) were diluted with RPMI 1640 culture medium to final concentrations of 0.625, 1.25, 2.5, 5, 10, and 20 μM.

Techniques: Activity Assay, Colorimetric Assay, CCK-8 Assay, Proliferation Assay, Staining, Control, Two Tailed Test

Different expression analysis in genes of canine mammary tumor cells treated with PAB. Canine mammary tumor U27 cells were seeded in 6-well plates and treated with PAB (2.5 μM) or an equivalent concentration of DMSO (vehicle control) for 24 h ( n = 3). (A) PCA score plot of the transcriptomics. (B) Number of differentially expressed genes (DEGs) upregulated and downregulated. (C) Volcano plot showing DEGs of RNA-seq data. (D) The heatmap displayed the upregulated DEGs and downregulated DEGs ranked by fold change.

Journal: Frontiers in Veterinary Science

Article Title: Pseudolaric acid B induces G2/M phase arrest in canine mammary tumor cells by targeting CDK1

doi: 10.3389/fvets.2025.1644200

Figure Lengend Snippet: Different expression analysis in genes of canine mammary tumor cells treated with PAB. Canine mammary tumor U27 cells were seeded in 6-well plates and treated with PAB (2.5 μM) or an equivalent concentration of DMSO (vehicle control) for 24 h ( n = 3). (A) PCA score plot of the transcriptomics. (B) Number of differentially expressed genes (DEGs) upregulated and downregulated. (C) Volcano plot showing DEGs of RNA-seq data. (D) The heatmap displayed the upregulated DEGs and downregulated DEGs ranked by fold change.

Article Snippet: Pseudolaric acid B, PAB (10 mM in DMSO) (#HY-N6939, MedChemExpress, USA) and Ro-3306 (10 mM) (#HY-12529, MCE, Shanghai, China) were diluted with RPMI 1640 culture medium to final concentrations of 0.625, 1.25, 2.5, 5, 10, and 20 μM.

Techniques: Expressing, Concentration Assay, Control, Transcriptomics, RNA Sequencing

Functional enrichment analysis of DEGs in canine mammary tumor cells treated with PAB. Canine mammary tumor U27 cells were seeded in 6-well plates and treated with PAB (2.5 μM) or an equivalent concentration of DMSO (vehicle control) for 24 h ( n = 3). (A) GO enrichment analysis of DEGs. (B) KEGG enrichment analysis of DEGs. The size of the dots represents the number of differential genes, and the intensity of the color represents the level of significance (the redder the color, the smaller the p -value) (C) PPI analysis of DEGs was performed using Cytoscape software. DEGs are arranged according to the betweenness centrality. The larger betweenness centrality, the larger the circle. (D) Validation of DEGs expression using RT-qPCR. GAPDH gene as a standardized internal reference. N = 3 for each group. Values represent mean ± SD of three biological replicates. Unpaired two-tailed Student’s t -test was performed. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant. FPRM, Fragments Per Kilobase of exon model per Million mapped fragments.

Journal: Frontiers in Veterinary Science

Article Title: Pseudolaric acid B induces G2/M phase arrest in canine mammary tumor cells by targeting CDK1

doi: 10.3389/fvets.2025.1644200

Figure Lengend Snippet: Functional enrichment analysis of DEGs in canine mammary tumor cells treated with PAB. Canine mammary tumor U27 cells were seeded in 6-well plates and treated with PAB (2.5 μM) or an equivalent concentration of DMSO (vehicle control) for 24 h ( n = 3). (A) GO enrichment analysis of DEGs. (B) KEGG enrichment analysis of DEGs. The size of the dots represents the number of differential genes, and the intensity of the color represents the level of significance (the redder the color, the smaller the p -value) (C) PPI analysis of DEGs was performed using Cytoscape software. DEGs are arranged according to the betweenness centrality. The larger betweenness centrality, the larger the circle. (D) Validation of DEGs expression using RT-qPCR. GAPDH gene as a standardized internal reference. N = 3 for each group. Values represent mean ± SD of three biological replicates. Unpaired two-tailed Student’s t -test was performed. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant. FPRM, Fragments Per Kilobase of exon model per Million mapped fragments.

Article Snippet: Pseudolaric acid B, PAB (10 mM in DMSO) (#HY-N6939, MedChemExpress, USA) and Ro-3306 (10 mM) (#HY-12529, MCE, Shanghai, China) were diluted with RPMI 1640 culture medium to final concentrations of 0.625, 1.25, 2.5, 5, 10, and 20 μM.

Techniques: Functional Assay, Concentration Assay, Control, Software, Biomarker Discovery, Expressing, Quantitative RT-PCR, Two Tailed Test

PAB induced G2/M cell cycle arrest by modulation of CDK1. (A) Molecular docking computer simulation predicts the binding site of PAB and CDK1 protein. (B) Western blot analysis of CDK1 protein expression under different concentrations (0, 0.625, 1.25, 2.5, 5 or 10 μM) for 24 h of PAB-treated canine mammary tumor U27 cells. β-Actin served as an internal reference and quantitative analysis of band intensities was performed using ImageJ software. (C,D) Representative western blot images of PAB (2.5 μM) and CDK1 from the CETSA (U27 cells, at 42 to 67 °C) and quantitatively analyzed using Image J software ( n = 3). (E) Canine mammary tumor U27 cells were exposed to different concentrations of Ro-0036 (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) for 24 h, the cell viability of canine mammary tumor U27 cells was detected by CCK-8 assay. (F–J) CDK1 downstream target genes ( BIRC5 , PLK1 , CDCA5 , RB1 , P53 ) mRNA expression levels were assayed after PAB or Ro-3306 treatment of U27 canine mammary tumor cells. Unpaired two-tailed Student’s t-test or one-way ANOVA was performed. (K–M) The cell cycle distribution of PAB-treated (0, 0.625, 1.25, 2.5, 5 or 10 μM) canine mammary tumor U27 cells for 12 h was determined by flow cytometry using DAPI staining.

Journal: Frontiers in Veterinary Science

Article Title: Pseudolaric acid B induces G2/M phase arrest in canine mammary tumor cells by targeting CDK1

doi: 10.3389/fvets.2025.1644200

Figure Lengend Snippet: PAB induced G2/M cell cycle arrest by modulation of CDK1. (A) Molecular docking computer simulation predicts the binding site of PAB and CDK1 protein. (B) Western blot analysis of CDK1 protein expression under different concentrations (0, 0.625, 1.25, 2.5, 5 or 10 μM) for 24 h of PAB-treated canine mammary tumor U27 cells. β-Actin served as an internal reference and quantitative analysis of band intensities was performed using ImageJ software. (C,D) Representative western blot images of PAB (2.5 μM) and CDK1 from the CETSA (U27 cells, at 42 to 67 °C) and quantitatively analyzed using Image J software ( n = 3). (E) Canine mammary tumor U27 cells were exposed to different concentrations of Ro-0036 (0, 0.625, 1.25, 2.5, 5, 10 or 20 μM) for 24 h, the cell viability of canine mammary tumor U27 cells was detected by CCK-8 assay. (F–J) CDK1 downstream target genes ( BIRC5 , PLK1 , CDCA5 , RB1 , P53 ) mRNA expression levels were assayed after PAB or Ro-3306 treatment of U27 canine mammary tumor cells. Unpaired two-tailed Student’s t-test or one-way ANOVA was performed. (K–M) The cell cycle distribution of PAB-treated (0, 0.625, 1.25, 2.5, 5 or 10 μM) canine mammary tumor U27 cells for 12 h was determined by flow cytometry using DAPI staining.

Article Snippet: Pseudolaric acid B, PAB (10 mM in DMSO) (#HY-N6939, MedChemExpress, USA) and Ro-3306 (10 mM) (#HY-12529, MCE, Shanghai, China) were diluted with RPMI 1640 culture medium to final concentrations of 0.625, 1.25, 2.5, 5, 10, and 20 μM.

Techniques: Binding Assay, Western Blot, Expressing, Software, CCK-8 Assay, Two Tailed Test, Flow Cytometry, Staining