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radicicol  (MedChemExpress)
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MedChemExpress radicicol
Fig. 7. Effects of LKB1 inhibition on naringenin-induced protein expression of oxidized myofibers in C2C12 myotubes. (a,b) Western Blot and quantification of MyHC isoform in C2C12 myotubes treated with naringenin combined with or without <t>Radicicol.</t> (c,d) Western Blot and quantification of p-LKB1, LKB1, p-AMPK, AMPK and PGC-1α in C2C12 myotubes treated with naringenin combined with or without Radicicol. Data are presented as mean ± SD, n = 5. *P < 0.05, **P < 0.01 as compared to the control group, #P < 0.05, ##P < 0.01 as compared to the naringenin treatment group.
Radicicol, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 7. Effects of LKB1 inhibition on naringenin-induced protein expression of oxidized myofibers in C2C12 myotubes. (a,b) Western Blot and quantification of MyHC isoform in C2C12 myotubes treated with naringenin combined with or without Radicicol. (c,d) Western Blot and quantification of p-LKB1, LKB1, p-AMPK, AMPK and PGC-1α in C2C12 myotubes treated with naringenin combined with or without Radicicol. Data are presented as mean ± SD, n = 5. *P < 0.05, **P < 0.01 as compared to the control group, #P < 0.05, ##P < 0.01 as compared to the naringenin treatment group.

Journal: Journal of Functional Foods

Article Title: Naringenin promotes the expression of oxidized myofibers via the PKA signaling pathway in C57BL/6J mice and C2C12 cells

doi: 10.1016/j.jff.2023.105902

Figure Lengend Snippet: Fig. 7. Effects of LKB1 inhibition on naringenin-induced protein expression of oxidized myofibers in C2C12 myotubes. (a,b) Western Blot and quantification of MyHC isoform in C2C12 myotubes treated with naringenin combined with or without Radicicol. (c,d) Western Blot and quantification of p-LKB1, LKB1, p-AMPK, AMPK and PGC-1α in C2C12 myotubes treated with naringenin combined with or without Radicicol. Data are presented as mean ± SD, n = 5. *P < 0.05, **P < 0.01 as compared to the control group, #P < 0.05, ##P < 0.01 as compared to the naringenin treatment group.

Article Snippet: Antibodies against Phospho-PKA (bs-4022R), PKA (bs-0520R) and Phospho-LKB1 (bs-3249R) were purchased from Bioss (Beijing, China); MYH7-specific Polyclonal antibody (22280–1-AP), β-Tubulin (10094–1-AP), MYH2-specific Polyclonal antibody (55069–1-AP), LKB1 (10746–1-AP), AMPK (10929–2-AP), PGC-1α (66369–1-Ig) and obtained all the secondary antibodies from Proteintech (Wuhan, China); Phospho-AMPK (# 2535) from Cell Signaling Technology (Beverly, Ma, USA); H-89 dihydrochloride (H-89; HY-15979A), Radicicol (HY-N6769) and Compound C (CC; HY-13418A) were purchased from MedChemExpress (New Jersey, USA).

Techniques: Inhibition, Expressing, Western Blot, Control