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sophoraflavanone g  (MedChemExpress)
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MedChemExpress sophoraflavanone g
Figure 3. Evaluation of estrogenic activity of the four prenylated flavonoids by Western blotting. MCF-7 cells were treated with 10 nM E2 (panels (A–D)), 100 nM prenylated flavonoids (kurarinone, kushenols A or I, or sophoraflavanone G) (panels (E–H,M–P) for Erk1/2, and panels (I–L,Q–T) for Akt), or with vehicle (0.1% DMSO; 0 min) in the absence (panels (A,B,E–L)) or presence (pan- els (C,D,M–T)) of 1 µM ICI for the indicated times (min). The cell extracts were then subjected to Western blotting. The relative amounts of phosphorylated protein to that of total protein are shown in the graphs. Data from three independent experiments were statistically evaluated using a t-test; * p < 0.05. E2: 17β-estradiol.
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Figure 3. Evaluation of estrogenic activity of the four prenylated flavonoids by Western blotting. MCF-7 cells were treated with 10 nM E2 (panels (A–D)), 100 nM prenylated flavonoids (kurarinone, kushenols A or I, or sophoraflavanone G) (panels (E–H,M–P) for Erk1/2, and panels (I–L,Q–T) for Akt), or with vehicle (0.1% DMSO; 0 min) in the absence (panels (A,B,E–L)) or presence (pan- els (C,D,M–T)) of 1 µM ICI for the indicated times (min). The cell extracts were then subjected to Western blotting. The relative amounts of phosphorylated protein to that of total protein are shown in the graphs. Data from three independent experiments were statistically evaluated using a t-test; * p < 0.05. E2: 17β-estradiol.

Journal: Genes

Article Title: Estrogenic Prenylated Flavonoids in Sophora flavescens .

doi: 10.3390/genes15020204

Figure Lengend Snippet: Figure 3. Evaluation of estrogenic activity of the four prenylated flavonoids by Western blotting. MCF-7 cells were treated with 10 nM E2 (panels (A–D)), 100 nM prenylated flavonoids (kurarinone, kushenols A or I, or sophoraflavanone G) (panels (E–H,M–P) for Erk1/2, and panels (I–L,Q–T) for Akt), or with vehicle (0.1% DMSO; 0 min) in the absence (panels (A,B,E–L)) or presence (pan- els (C,D,M–T)) of 1 µM ICI for the indicated times (min). The cell extracts were then subjected to Western blotting. The relative amounts of phosphorylated protein to that of total protein are shown in the graphs. Data from three independent experiments were statistically evaluated using a t-test; * p < 0.05. E2: 17β-estradiol.

Article Snippet: Genes 2024, 15, 204 3 of 15Genes 2024, 15, x FOR PEER REVIEW of 15 Kurarinone was purchased from Sigma-Aldrich (St. Louis, MO, USA), kushenol A from BioBioPha (Kunming, China), and kushenol I and sophoraflavanone G from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Activity Assay, Western Blot

Figure 4. Evaluation of estrogenic activity of the four prenylated flavonoids (100 nM) by real-time RT-PCR. (A) The results of real-time RT-PCR using the designated 30 estrogen-responsive genes for E2 (a), kurarinone (b), kushenol A (c), kushenol I (d), and sophoraflavanone G (e). The graphs show the expression level of each estrogen-responsive gene (the name is shown at the bottom), obtained via real-time RT-PCR, and the bars show the mean ± SD (n = 3) of log2-transformed ratios of the data obtained in the presence of each chemical to those of data acquired in their absence (E2+/E2−, for example) in the Y axis. * p < 0.05: between each compound and the control (vehicle; 0.1% DMSO). (B) Correlation analysis of the profiles obtained by real-time RT-PCR. The gene expression profiles for the set of 30 estrogen-responsive genes were compared between E2 and the four prenylated flavonoids (panels a–d), or within the four prenylated flavonoids (panels e–j), based on linear regression, and the results were visualized as scatter plot graphs. The vertical and horizontal axes indicate log2 values for the indicated chemicals. The correlation coefficients (R-values) and the values for the significance of correlation (p-values) are indicated. E2: 17β-estradiol.

Journal: Genes

Article Title: Estrogenic Prenylated Flavonoids in Sophora flavescens .

doi: 10.3390/genes15020204

Figure Lengend Snippet: Figure 4. Evaluation of estrogenic activity of the four prenylated flavonoids (100 nM) by real-time RT-PCR. (A) The results of real-time RT-PCR using the designated 30 estrogen-responsive genes for E2 (a), kurarinone (b), kushenol A (c), kushenol I (d), and sophoraflavanone G (e). The graphs show the expression level of each estrogen-responsive gene (the name is shown at the bottom), obtained via real-time RT-PCR, and the bars show the mean ± SD (n = 3) of log2-transformed ratios of the data obtained in the presence of each chemical to those of data acquired in their absence (E2+/E2−, for example) in the Y axis. * p < 0.05: between each compound and the control (vehicle; 0.1% DMSO). (B) Correlation analysis of the profiles obtained by real-time RT-PCR. The gene expression profiles for the set of 30 estrogen-responsive genes were compared between E2 and the four prenylated flavonoids (panels a–d), or within the four prenylated flavonoids (panels e–j), based on linear regression, and the results were visualized as scatter plot graphs. The vertical and horizontal axes indicate log2 values for the indicated chemicals. The correlation coefficients (R-values) and the values for the significance of correlation (p-values) are indicated. E2: 17β-estradiol.

Article Snippet: Genes 2024, 15, 204 3 of 15Genes 2024, 15, x FOR PEER REVIEW of 15 Kurarinone was purchased from Sigma-Aldrich (St. Louis, MO, USA), kushenol A from BioBioPha (Kunming, China), and kushenol I and sophoraflavanone G from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Activity Assay, Quantitative RT-PCR, Expressing, Transformation Assay, Control, Gene Expression

Figure 5. Evaluation of estrogenic activity of the four prenylated flavonoids (1 µM) by real-time RT-PCR. (A) The results of real-time RT-PCR (n = 3) for each of the designated 30 estrogen-responsive genes for E2 (a), kurarinone (b), kushenol A (c), kushenol I (d), and sophoraflavanone G (e). For details, see Figure 4 legend. (B) Correlation analysis of the profiles obtained by real-time RT-PCR. The gene expression profiles for the set of 30 estrogen-responsive genes were compared between E2 and the four prenylated flavonoids (panels a–d), or within the four prenylated flavonoids (panels e–j), based on linear regression, and the results were visualized as scatter plot graphs. The vertical and horizontal axes indicate log2 values for the indicated chemicals. The correlation coefficients (R-values) and the values for the significance of correlation (p-values) are indicated. E2: 17β-estradiol.

Journal: Genes

Article Title: Estrogenic Prenylated Flavonoids in Sophora flavescens .

doi: 10.3390/genes15020204

Figure Lengend Snippet: Figure 5. Evaluation of estrogenic activity of the four prenylated flavonoids (1 µM) by real-time RT-PCR. (A) The results of real-time RT-PCR (n = 3) for each of the designated 30 estrogen-responsive genes for E2 (a), kurarinone (b), kushenol A (c), kushenol I (d), and sophoraflavanone G (e). For details, see Figure 4 legend. (B) Correlation analysis of the profiles obtained by real-time RT-PCR. The gene expression profiles for the set of 30 estrogen-responsive genes were compared between E2 and the four prenylated flavonoids (panels a–d), or within the four prenylated flavonoids (panels e–j), based on linear regression, and the results were visualized as scatter plot graphs. The vertical and horizontal axes indicate log2 values for the indicated chemicals. The correlation coefficients (R-values) and the values for the significance of correlation (p-values) are indicated. E2: 17β-estradiol.

Article Snippet: Genes 2024, 15, 204 3 of 15Genes 2024, 15, x FOR PEER REVIEW of 15 Kurarinone was purchased from Sigma-Aldrich (St. Louis, MO, USA), kushenol A from BioBioPha (Kunming, China), and kushenol I and sophoraflavanone G from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Activity Assay, Quantitative RT-PCR, Gene Expression