hy-n0862 Search Results


harringtonine ht  (MedChemExpress)
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MedChemExpress harringtonine ht
a Polysome profiles from lysates with RNase A treatment of NC and SMYD5 KO1 Huh7 cell lines. Black arrows denote the disomes. b , c WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.001–1 mg/L, 15 min) or <t>Harringtonine</t> (HT) (0.1–10 μM, 15 min). d , e WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.01–1 mg/L, 15 min) or HT(10 μM, 15 min). Before ANS or HT treatment, the NC and SMYD5 KO1 Huh7 cell lines were treated with DMSO or ZAK inhibitor M443 (5 μM, 1 h) as indicated. Note: α-Tubulin was used as a control for panels in all above and below WB analyses.
Harringtonine Ht, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Polysome profiles from lysates with RNase A treatment of NC and SMYD5 KO1 Huh7 cell lines. Black arrows denote the disomes. b , c WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.001–1 mg/L, 15 min) or Harringtonine (HT) (0.1–10 μM, 15 min). d , e WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.01–1 mg/L, 15 min) or HT(10 μM, 15 min). Before ANS or HT treatment, the NC and SMYD5 KO1 Huh7 cell lines were treated with DMSO or ZAK inhibitor M443 (5 μM, 1 h) as indicated. Note: α-Tubulin was used as a control for panels in all above and below WB analyses.

Journal: Cell Research

Article Title: SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma

doi: 10.1038/s41422-024-01013-3

Figure Lengend Snippet: a Polysome profiles from lysates with RNase A treatment of NC and SMYD5 KO1 Huh7 cell lines. Black arrows denote the disomes. b , c WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.001–1 mg/L, 15 min) or Harringtonine (HT) (0.1–10 μM, 15 min). d , e WB analyses for phosphorylation of p38 in the NC and SMYD5 KO1 Huh7 cell lines treated with ANS (0.01–1 mg/L, 15 min) or HT(10 μM, 15 min). Before ANS or HT treatment, the NC and SMYD5 KO1 Huh7 cell lines were treated with DMSO or ZAK inhibitor M443 (5 μM, 1 h) as indicated. Note: α-Tubulin was used as a control for panels in all above and below WB analyses.

Article Snippet: For activating the phosphorylation of p38 and inhibiting ZAK in cells, anisomycin (ANS), Homoharringtonine (HHT), harringtonine (HT), Menadione (Mena), and M443 were obtained from MCE (HY-18982, HY-N0862, HY-14944, HY-B0332 and HY-112274).

Techniques: Phospho-proteomics, Control