Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on cell viability in human breast cancer cells. ( A ) The chemical structure of alisol A. ( B ) Effects of alisol A on cell viability in MDA-MB-231, MDA-MB-453, and MCF-7 cells. Cells were treated with different concentrations of alisol A for 24 h. Then, cell viability was quantified by the MTT assay. Data represent the mean ± S.D of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: MTT Assay

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on induction of cell apoptosis. ( A ) Quantification of apoptotic cells was performed by flow cytometer. MDA-MB-231 cells were treated with different concentrations of alisol A for 24 h. Cells were stained with Annexin-V-FITC/7AAD according to the manufacturer’s instructions. ( B ) Effects of alisol A on the expression of caspases in the MDA-MB-231 cells. The cells were treated with alisol A for 24 h. The cell lysates were collected and subjected to Western blotting analysis. Results are representative of at least three independent experiments showing similar results.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Flow Cytometry, Staining, Expressing, Western Blot

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on induction of autophagy. ( A ) Autophagy in alisol A-treated MDA-MB-231 cells were stained with AO and examined under a fluorescence microscope. Quantitation of formation of autophagolysosomes in alisol A-treated cancer cells was performed. ( B ) Western blotting analysis of the protein expression of LC3 in MDA-MB-231 cells treated with alisol A for 12 h. Data are expressed as mean ± S.D of at least three independent experiments. ** p < 0.01.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Staining, Fluorescence, Microscopy, Quantitation Assay, Western Blot, Expressing

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of 3-MA on alisol A-induced autophagy. ( A ) Autophagy inhibitor 3-MA reversed the cytotoxic effects induced by alisol A. MDA-MB-231 cells were pre-incubated with or without 3-MA (5 mM) for 2 h and then incubated with alisol A (20 µM) for another 24 h, after which the cells were subjected to MTT assay. ( B ) Autophagy in alisol A-treated MDA-MB-231 cells were inhibited by 3-MA. Quantitation of formation of autophagolysosomes in treated cancer cells was performed. Data are expressed as mean ± S.D of at least three independent experiments. * p < 0.05, *** p < 0.001.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Incubation, MTT Assay, Quantitation Assay

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on cell cycle in MDA-MB-231 cells. ( A ) MDA-MB-231 cells were treated with alisol A for 24 h, fixed in 70% ethanol at 4 °C overnight, and stained with propidium iodide (PI). Cell cycle distribution was assessed by flow cytometry. Quantified histograms display the effects of alisol A on cell cycle distribution. ( B ) The expression of cell cycle-related proteins, Cyclin D1 and p21 in MDA-MB-231 cells were analyzed by Western blotting. Data are presented as the mean ± S.D of three independent experiments. * p < 0.05, *** p < 0.001.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Staining, Flow Cytometry, Expressing, Western Blot

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on autophagy related signaling pathways. The cells were treated with alisol A for 24 h. The cell lysates were collected and subjected to Western blotting analysis to detect the expression of proteins in cancer cells. Results are representative of at least three independent experiments showing similar results.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Protein-Protein interactions, Western Blot, Expressing

Journal: Molecules

Article Title: Alisol A Suppresses Proliferation, Migration, and Invasion in Human Breast Cancer MDA-MB-231 Cells

doi: 10.3390/molecules24203651

Figure Lengend Snippet: Effects of alisol A on cell migration and invasion. ( A ) The effects of alisol A on cell migration was measured by wound healing assay. ( B ) Transwell migration (left) and invasion (right) assay was used to evaluate the in vitro anti-metastatic effects of alisol A. ( C ) Effects of alisol A on MMP-9 activity by gelatin zymography assay. ( D ) Effects of alisol A on MMP-2 and MMP-9 protein expression in MDA-MB-231 cells. Data are presented as the mean ± S.D of three independent experiments. ** p < 0.01, *** p < 0.001.

Article Snippet: Alisol A was purchased from MedChemExpress (Monmouth Junction, NJ, USA) (The chemical structure is shown in A).

Techniques: Migration, Wound Healing Assay, In Vitro, Activity Assay, Zymography Assay, Expressing