hy-n0770 Search Results


exogenous ils  (MedChemExpress)
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MedChemExpress exogenous ils
(A) CCK-8 assay assessing cytotoxicity <t>of</t> <t>isoliensinine</t> <t>(ILS).</t> Cell viability remained unaffected at 5 μM ILS, confirming its non-toxic concentration for subsequent experiments. (B-D) qPCR analysis of hypertrophic markers: ANP, BNP, and β-MHC. mRNA levels were normalized to β-actin. (E-F) Phalloidin staining (red: F-actin; blue: nuclei) and quantitative analysis of cell surface area. (G-I) Fluorescence imaging and quantification of intracellular ROS (DHE probe, red fluorescence) and mitochondrial superoxide (MitoSOX Green probe, green fluorescence). (J-M) Western blot analysis of apoptosis-related proteins: Caspase3, Bax, Bcl-2, and β-actin (loading control). Data presented as mean ± SEM (n ≥ 3), *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
Exogenous Ils, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n0770/Isoliensinine/bio_rxiv__2025__07__07__663610-48-9-12
Average 93 stars, based on 1 article reviews
exogenous ils - by Bioz Stars, 2026-10
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(A) CCK-8 assay assessing cytotoxicity of isoliensinine (ILS). Cell viability remained unaffected at 5 μM ILS, confirming its non-toxic concentration for subsequent experiments. (B-D) qPCR analysis of hypertrophic markers: ANP, BNP, and β-MHC. mRNA levels were normalized to β-actin. (E-F) Phalloidin staining (red: F-actin; blue: nuclei) and quantitative analysis of cell surface area. (G-I) Fluorescence imaging and quantification of intracellular ROS (DHE probe, red fluorescence) and mitochondrial superoxide (MitoSOX Green probe, green fluorescence). (J-M) Western blot analysis of apoptosis-related proteins: Caspase3, Bax, Bcl-2, and β-actin (loading control). Data presented as mean ± SEM (n ≥ 3), *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Journal: bioRxiv

Article Title: Isoliensinine Ameliorates Pathological Cardiac Hypertrophy After Aerobic Exercise by Activating Cytochrome c Oxidase Subunit 6A2

doi: 10.1101/2025.07.07.663610

Figure Lengend Snippet: (A) CCK-8 assay assessing cytotoxicity of isoliensinine (ILS). Cell viability remained unaffected at 5 μM ILS, confirming its non-toxic concentration for subsequent experiments. (B-D) qPCR analysis of hypertrophic markers: ANP, BNP, and β-MHC. mRNA levels were normalized to β-actin. (E-F) Phalloidin staining (red: F-actin; blue: nuclei) and quantitative analysis of cell surface area. (G-I) Fluorescence imaging and quantification of intracellular ROS (DHE probe, red fluorescence) and mitochondrial superoxide (MitoSOX Green probe, green fluorescence). (J-M) Western blot analysis of apoptosis-related proteins: Caspase3, Bax, Bcl-2, and β-actin (loading control). Data presented as mean ± SEM (n ≥ 3), *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: 3) To validate the functional role of isoliensinine (ILS), exogenous ILS (HY-N0770, MCE, USA) was administered to assess its effects on cardiac structure and function in TAC mice.

Techniques: CCK-8 Assay, Concentration Assay, Staining, Fluorescence, Imaging, Western Blot, Control