Journal: Bioscience Reports

Article Title: Effects of rhBMP-2 gene transfection to periodontal ligament cells on osteogenesis

doi: 10.1042/BSR20160585

Figure Lengend Snippet: M: DNA ladder marker with relative molecular weight 1 kbp; (1) enzyme-digested product 5.4 kbp (pcDNA3.1) and 1.2 kbp (target gene); (2) recombinant plasmid pcDNA3.1-rhBMP-2 (6.6 kbp).

Article Snippet: The protein separated by electrophoresis was transformed into the PVDF membrane and 1:600 rhBMP-2 rabbit anti-human monoclonal antibody (Wuhan Boster Biological Technology Ltd., Wuhan, Hubei, China) was added into the blocked PVDF membrane.

Techniques: Marker, Molecular Weight, Recombinant, Plasmid Preparation

Journal: medRxiv

Article Title: Altered IgG4 Antibody Response to Repeated mRNA versus Protein COVID Vaccines

doi: 10.1101/2024.01.17.24301374

Figure Lengend Snippet: Randomly selected serum samples from COVID vaccine recipients included participants from 2019nCoV-307 ( ClinicalTrials.gov : NCT05463068 ) who received three homologous doses of Moderna (mRNA-1273, blue, n = 10) or Pfizer (BNT162b2, teal, n = 10) mRNA vaccine followed by one dose of Novavax (NVX-CoV2373), and from 2019nCoV-301 ( ClinicalTrials.gov : NCT04611802 ) who received four homologous doses of Novavax (purple, n = 18). Serum was collected ≥6 months after the last dose for homologous three-dose samples (open circle) and ∼4 weeks after fourth dose samples (filled circle). For each vaccine type, the GMFR between the third and fourth doses is indicated in bolded text above the fourth-dose data points. (A) Serum concentrations of anti–ancestral (Wuhan) rS–specific total IgG, IgG1, and IgG4 were measured using quantitative ELISA and reported as GMT with 95% CI. One subject (Moderna) was excluded due to a failed logic check. (B) ADCP, ADCC, and ADCD were analyzed using previously described surrogate SARS-CoV-2 ancestral rS–specific antibody Fc functional multiplex assays assessing median fluorescence intensity of FcγRIIa, FcγRIIIa, and C1q binding, respectively. One subject (Novavax) was excluded due to missing data (x3 Dose + Novavax). SAS ® 9.4 software (SAS Institute Inc., Cary, NC), PROC SGPLOT was used to detect far-outliers based on the log-transformed assay values, defined as Far-outliers = Observations > Q3 + 3*IQR or < Q1 – 3*IQR; none were found or excluded. Abbreviations: ADCC, antibody-dependent cellular cytotoxicity; ADCD, antibody-dependent complement deposition; ADCP, antibody-dependent cellular phagocytosis; CI, confidence interval; C1q complement component 1q; ELISA, enzyme-linked immunosorbent assay; FcγRIIa, Fcγ Receptor IIa; FcγRIIIa, Fcγ Receptor IIIa; GMFR, geometric mean fold rise; GMT, geometric mean titer; IgG, Immunoglobulin G; MFI, median fluorescent intensity; rS, recombinant spike protein; 3x, three-dose.

Article Snippet: The reference standards for ELISA were as follows; SARS-CoV-2 Spike RBD human IgG monoclonal antibody (Acro Biosystems, Cat# RAS009-C02) for total IgG, anti-SARS-CoV-2 Spike RBD human IgG1 monoclonal antibody (Acro Biosystems, Cat# SPD-M265) for IgG1, anti-SARS-CoV-2 spike RBD human IgG2 monoclonal antibody (Acro Biosystems, Cat# SPD-M400a), anti-SARS-CoV-2 spike RBD human IgG3 monoclonal antibody (Acro Biosystems, Cat# SPD-M401a), and anti-SARS-CoV-2 Spike RBD human IgG4 monoclonal antibody (Acro Biosystems, Cat# SPD-M402a) for IgG4.

Techniques: Enzyme-linked Immunosorbent Assay, Functional Assay, Multiplex Assay, Fluorescence, Binding Assay, Software, Transformation Assay, Recombinant