Journal: Molecular Therapy. Nucleic Acids
Article Title: Genetic Alphabet Expansion Provides Versatile Specificities and Activities of Unnatural-Base DNA Aptamers Targeting Cancer Cells
Figure Lengend Snippet: Cancer Cell Imaging by the Ds-DNA Aptamers A variety of cancer cell lines (MCF7, MDA-MB-231, T-47D, MIA PaCa-2, PC-3, HeLa, A549, and CCRF-CEM), as well as normal cell lines (MCF10A and HUVEC), were incubated with 250 nM each of Alexa 488-labeled Ds-DNA aptamer and AS1411-26 (as a control) at 4°C for 30 min and then analyzed by fluorescent microscopy.
Article Snippet: For the specificity profiling of the obtained aptamers, other types of cell lines—MDA-MB-453 (BRC, #RCB1192), HCT-116 (BRC, #RCB2979), A549 (BRC, #RCB0098), MKN45 (BRC, #RCB1001), NIH:OVCAR-3 (BRC, #RCB2135), PC-3 (ATCC, #CRL-1435), MIA PaCa-2 (from the Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer (IDAC), Tohoku University, #TKG0227), Panc-1 (IDAC, #TKG0606), PLC/PRF/5 (from the Japanese Collection of Research Bioresources Cell Bank, #JCRB0406), HeLa (BRC, #RCB0007), KG-1 (BRC, #RCB1166), CCRF-CEM (IDAC, #I-4924), and HUVEC (Lonza, #CC-2519)—were also cultured according to the suppliers’ instructions and used for evaluations of the aptamers’ binding.
Techniques: Imaging, Multiple Displacement Amplification, Incubation, Labeling, Microscopy