human umbilical artery smooth muscle cells Search Results


huasmc  (PromoCell)
94
PromoCell huasmc
a, b. G-baToN can detect cancer cell-endothelial cell (EC) interactions. HUVECs expressing αGFP were co-cultured with or without Tomato pos sGFP-expressing lung cancer sender cells at a 1:1 ratio for 24 hours. ( a ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either <t>control</t> <t>HUVEC</t> receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( b ) MFI of GFP on PI neg Tomato neg BFP pos CD31 pos Receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. c , d. G-baToN can detect endothelial cell (EC)-smooth muscle cell (SMC) interactions. Primary human umbilical artery smooth muscle cells <t>(HUASMC)</t> expressing αGFP were co-cultured with or without sGFP-expressing HUVEC senders cells at a 1:1 ratio for 24 hours. ( c ) Representative images of sGFP-expressing HUVEC sender cells co-cultured with either control HUASMC receiver cells (expressing BFP) or αGFP HUASMC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( d ) MFI of GFP on PI neg BFP pos receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. e , f , g. G-baToN can detect cancer cell-endothelial cell (EC) interactions in 3D-microfluidic culture. ( e ) Details on design of 3D-microfluidic devices for cancer cell-endothelial cell co-culture. ( f ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:10 ratio for 24 hours. Scale bars = 200 μm. ( g ) Average number of GFP pos hUVEC after co-culture with cancer cells for 24 h. 10 areas from three chips with 200X magnification were used for the quantification. ** p<0.01, n = 10.
Huasmc, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human umbilical artery  (Cell Applications Inc)
93
Cell Applications Inc human umbilical artery
a, b. G-baToN can detect cancer cell-endothelial cell (EC) interactions. HUVECs expressing αGFP were co-cultured with or without Tomato pos sGFP-expressing lung cancer sender cells at a 1:1 ratio for 24 hours. ( a ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either <t>control</t> <t>HUVEC</t> receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( b ) MFI of GFP on PI neg Tomato neg BFP pos CD31 pos Receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. c , d. G-baToN can detect endothelial cell (EC)-smooth muscle cell (SMC) interactions. Primary human umbilical artery smooth muscle cells <t>(HUASMC)</t> expressing αGFP were co-cultured with or without sGFP-expressing HUVEC senders cells at a 1:1 ratio for 24 hours. ( c ) Representative images of sGFP-expressing HUVEC sender cells co-cultured with either control HUASMC receiver cells (expressing BFP) or αGFP HUASMC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( d ) MFI of GFP on PI neg BFP pos receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. e , f , g. G-baToN can detect cancer cell-endothelial cell (EC) interactions in 3D-microfluidic culture. ( e ) Details on design of 3D-microfluidic devices for cancer cell-endothelial cell co-culture. ( f ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:10 ratio for 24 hours. Scale bars = 200 μm. ( g ) Average number of GFP pos hUVEC after co-culture with cancer cells for 24 h. 10 areas from three chips with 200X magnification were used for the quantification. ** p<0.01, n = 10.
Human Umbilical Artery, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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huasmc p10964  (Innoprot Inc)
93
Innoprot Inc huasmc p10964
a, b. G-baToN can detect cancer cell-endothelial cell (EC) interactions. HUVECs expressing αGFP were co-cultured with or without Tomato pos sGFP-expressing lung cancer sender cells at a 1:1 ratio for 24 hours. ( a ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either <t>control</t> <t>HUVEC</t> receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( b ) MFI of GFP on PI neg Tomato neg BFP pos CD31 pos Receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. c , d. G-baToN can detect endothelial cell (EC)-smooth muscle cell (SMC) interactions. Primary human umbilical artery smooth muscle cells <t>(HUASMC)</t> expressing αGFP were co-cultured with or without sGFP-expressing HUVEC senders cells at a 1:1 ratio for 24 hours. ( c ) Representative images of sGFP-expressing HUVEC sender cells co-cultured with either control HUASMC receiver cells (expressing BFP) or αGFP HUASMC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( d ) MFI of GFP on PI neg BFP pos receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. e , f , g. G-baToN can detect cancer cell-endothelial cell (EC) interactions in 3D-microfluidic culture. ( e ) Details on design of 3D-microfluidic devices for cancer cell-endothelial cell co-culture. ( f ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:10 ratio for 24 hours. Scale bars = 200 μm. ( g ) Average number of GFP pos hUVEC after co-culture with cancer cells for 24 h. 10 areas from three chips with 200X magnification were used for the quantification. ** p<0.01, n = 10.
Huasmc P10964, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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human umbilical artery vascular smooth muscle cells (vsmcs)  (Lonza)
90
Lonza human umbilical artery vascular smooth muscle cells (vsmcs)
Microcontact printing used to provide spatial cues <t>for</t> <t>vascular</t> tissue self-organization. (A) Phase contrast images of <t>VSMCs</t> micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).
Human Umbilical Artery Vascular Smooth Muscle Cells (Vsmcs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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human umbilical artery smooth muscle cells (uasmc)  (Lonza)
90
Lonza human umbilical artery smooth muscle cells (uasmc)
Microcontact printing used to provide spatial cues <t>for</t> <t>vascular</t> tissue self-organization. (A) Phase contrast images of <t>VSMCs</t> micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).
Human Umbilical Artery Smooth Muscle Cells (Uasmc), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human umbilical artery smooth muscle cells (uasmc)/product/Lonza
Average 90 stars, based on 1 article reviews
human umbilical artery smooth muscle cells (uasmc) - by Bioz Stars, 2025-12
90/100 stars
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Image Search Results


a, b. G-baToN can detect cancer cell-endothelial cell (EC) interactions. HUVECs expressing αGFP were co-cultured with or without Tomato pos sGFP-expressing lung cancer sender cells at a 1:1 ratio for 24 hours. ( a ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( b ) MFI of GFP on PI neg Tomato neg BFP pos CD31 pos Receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. c , d. G-baToN can detect endothelial cell (EC)-smooth muscle cell (SMC) interactions. Primary human umbilical artery smooth muscle cells (HUASMC) expressing αGFP were co-cultured with or without sGFP-expressing HUVEC senders cells at a 1:1 ratio for 24 hours. ( c ) Representative images of sGFP-expressing HUVEC sender cells co-cultured with either control HUASMC receiver cells (expressing BFP) or αGFP HUASMC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( d ) MFI of GFP on PI neg BFP pos receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. e , f , g. G-baToN can detect cancer cell-endothelial cell (EC) interactions in 3D-microfluidic culture. ( e ) Details on design of 3D-microfluidic devices for cancer cell-endothelial cell co-culture. ( f ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:10 ratio for 24 hours. Scale bars = 200 μm. ( g ) Average number of GFP pos hUVEC after co-culture with cancer cells for 24 h. 10 areas from three chips with 200X magnification were used for the quantification. ** p<0.01, n = 10.

Journal: bioRxiv

Article Title: G-baToN: a versatile reporter system for cancer cell-stromal cell interactions

doi: 10.1101/2020.02.01.929877

Figure Lengend Snippet: a, b. G-baToN can detect cancer cell-endothelial cell (EC) interactions. HUVECs expressing αGFP were co-cultured with or without Tomato pos sGFP-expressing lung cancer sender cells at a 1:1 ratio for 24 hours. ( a ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( b ) MFI of GFP on PI neg Tomato neg BFP pos CD31 pos Receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. c , d. G-baToN can detect endothelial cell (EC)-smooth muscle cell (SMC) interactions. Primary human umbilical artery smooth muscle cells (HUASMC) expressing αGFP were co-cultured with or without sGFP-expressing HUVEC senders cells at a 1:1 ratio for 24 hours. ( c ) Representative images of sGFP-expressing HUVEC sender cells co-cultured with either control HUASMC receiver cells (expressing BFP) or αGFP HUASMC receiver cells at a 1:1 ratio for 24 hours. Scale bars = 50 μm. ( d ) MFI of GFP on PI neg BFP pos receiver cells was assessed by flow cytometry and is shown as mean +/- SD of triplicate cultures. ** p<0.01, n=3. e , f , g. G-baToN can detect cancer cell-endothelial cell (EC) interactions in 3D-microfluidic culture. ( e ) Details on design of 3D-microfluidic devices for cancer cell-endothelial cell co-culture. ( f ) Representative images of Tomato pos sGFP-expressing lung cancer sender cells co-cultured with either control HUVEC receiver cells (HUVECs expressing BFP) or αGFP HUVEC receiver cells at a 1:10 ratio for 24 hours. Scale bars = 200 μm. ( g ) Average number of GFP pos hUVEC after co-culture with cancer cells for 24 h. 10 areas from three chips with 200X magnification were used for the quantification. ** p<0.01, n = 10.

Article Snippet: HEK-293T, B16-F10, A549, H460 and HUVEC cells were originally purchased from ATCC; HUASMC were purchased from PromoCell (C-12500); H82 cells were kindly provided by Julien Sage (Stanford School of Medicine); KP (238N1) and KPT (2985T2) lung adenocarcinoma cells were generated in the Winslow Lab. HEK-293T, 238N1, 2985T2 and B16-F10 cells were cultured in DMEM containing 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin.

Techniques: Expressing, Cell Culture, Flow Cytometry, Co-Culture Assay

Microcontact printing used to provide spatial cues for vascular tissue self-organization. (A) Phase contrast images of VSMCs micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).

Journal: Integrative biology : quantitative biosciences from nano to macro

Article Title: Vascular Smooth Muscle Contractility Depends on Cell Shape

doi: 10.1039/c1ib00061f

Figure Lengend Snippet: Microcontact printing used to provide spatial cues for vascular tissue self-organization. (A) Phase contrast images of VSMCs micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).

Article Snippet: Human umbilical artery vascular smooth muscle cells (VSMCs) were purchased from Lonza (Walkersville, MD) at passage 3 and cultured in growth medium consisting of M199 culture medium (GIBCO, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen), 10 mM HEPES (GIBCO, Invitrogen, Carlsbad, CA), 3.5 g/L glucose, 2mg/L vitamin B-12, 50 U/ml penicillin and 50 U/mL streptomycin (GIBCO).

Techniques: Staining