Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: Inhibition of cologenecity in colorectal cancer cells by NVD. (Clonogenic assay; 7 days). a , b NVD administration (40 and 60 μM) of HCT116 and HT29 cells inhibiting colony formation. Each value represents a mean ± SD (n = 3)

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Inhibition, Clonogenic Assay

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: a , b NVD treatment on HCT116 and HT29 cells resulted in accumulation of cells at the G2-phase and growth inhibition and apoptosis. After 24 h incubation of NVD treated cells and staining with propidium iodide, DNA content was analyzed by flow cytometry. c , d NVD treatment on HCT116 and HT29 cells resulted in accumulation of cells at the G2-phase. After 24 h incubation of NVD treated cells and staining with propidium iodide, DNA content was analyzed by flow cytometry. Percentage of cell population in G2-phase of the cell cycle is shown. Experiments were performed in triplicate

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Inhibition, Incubation, Staining, Flow Cytometry

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: Percentage of cell population after administration of NVD

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques:

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: Effect of NVD treatment of HCT116 and HT29 ( a , b ) cells on protein expression of cdc2, WAF1/p21, cdk 2, 4 cyclin A, B1, E2 and ( c , d ) showing active Caspase 3, 7, 9 and PARP. Cells were administrated with NVD (20, 40 and 60 μM) for 24 h and 48 h and harvested. Total cell lysates were prepared and 40 μM proteins was subjected to SDS page followed by immunoblot analysis and chemiluminescence detection. Equal loading of protein was verified by stripping the Immunoblot and again probing it for Actin. The immunoblots shown here are representative of three individual experiments with similar results

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Expressing, SDS Page, Western Blot, Stripping Membranes

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: a Effect of treatment of NVD to HCT116 and HT29 cell lines on protein expression of Bax, Bcl2, Bak and Bcl-X L . b Immunoblot analysis of β-catenin expression of HCT116 and HT29 in NVD treated group as compared to control group. The cells were administrated with NVD for 48 h and harvested and cell lysates prepared. The data are representative of three independent experiments with similar results

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Expressing, Western Blot, Control

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: a Modulation of β -catenin expression by NVD treatment in HCT116 ( a ) and HT29 ( b ) cells. mRNA expression of β-catenin in NVD treated HCT116 and HT29 cells (RT-PCR), experiment performed in triplicate (mean ± SD), **p < 0.01. b Effect of NVD on protein expression of β-catenin, Survivin and phosphorylation of Akt at Ser473 in HCT116 and HT29 cells., Total cell lysate were prepared and 40 μM proteins was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-Actin

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Phospho-proteomics, SDS Page, Western Blot, Stripping Membranes

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: Imunofluorescence staining of HCT116 ( b ) and HT29 ( d ) demonstrating expression of β-catenin in both NVD treated (60 µM) as compared to control HCT116 ( a ) and HT29 ( c ) cells (untreated). Alexa fluor staining of β-catenin of both cell lines (green fluorescence) and counter stained with DAPI (blue fluorescence) were observed

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Staining, Expressing, Control, Fluorescence

Journal: Cell & Bioscience

Article Title: Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

doi: 10.1186/s13578-019-0277-z

Figure Lengend Snippet: Western blot analysis of cellular lysates prepared from HCT116 and HT29 cells. Lysates prepared from HCT116 and HT29 cells, initially seeded at density of 1.5 × 10 4 cells were administrated with 20, 40 and 60 µM concentrations of NVD. The blots probed with CK2 αα’, PI3-K, STAT3, STAT P-Try705, NFκB p65 P-S529, IκBα P-S32/36 and xIAP antibodies were shown. Actin blots are shown as loading controls. Data based on three different experiments, each carried out in triplicate

Article Snippet: Two human colorectal cancer cell lines HCT116 and HT29 (obtained from College of Pharmacy, King Saud University, KSA) were cultured in a 5% CO 2 atmosphere at 37 °C in medium containing Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC ® 30–2002TM), 10% fetal bovine serum (FBS, Gibco) as well as 1% penicillin/streptomycin.

Techniques: Western Blot