human tgf-β1 Search Results


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    R&D Systems tgf β1
    Monocyte chemoattractant protein‐1 mediates tumor growth factor <t>β1‐primed</t> fibrogenic activation of hepatic stellate cells. (A) Experimental approach to analyze the fibrogenic activation of hSCs. LX‐2 cells were treated with <t>TGF‐ß1</t> for 1 hour and then supplied with fresh medium. MCP‐1 concentration in the culture supernatant was assessed at the indicated times. During cell cultivation, the culture medium was changed daily with or without the presence of MCP‐1 blocking antibody. At the end of the culturing phase, the cell count and type IV collagen protein levels were analyzed. (B) Assessment of extracellular secretion of MCP‐1 in the culture supernatant. Data represent mean ± SD. (C) Cell growth at 120 hours after transient TGF‐β1 stimulation (0, 10 ng/mL and 100 ng/mL) in the presence of either IgG control antibody or MCP‐1 blocking antibody ( # P
    Tgf β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf β1/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tgf β1 - by Bioz Stars, 2021-05
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    86
    R&D Systems recombinant human tgf β1
    Anti-fibrotic effects of IFN-γ-treated mesenchymal stem cells (MSCs) in the kidney of IRI rats. IFN-γ-treated or untreated rat MSCs were injected immediately after IRI induction. Twenty-one days later, protein levels of fibrosis markers were evaluated by western blot ( a ) and immunohistochemical ( b , c ) analyses. ( a ) Western blot analysis of α-SMA and <t>TGF-β1</t> in the rat kidney cortex. Protein levels were normalized to GAPDH levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. ( b ) Representative immunohistochemical staining of α-SMA, Collagen type I (Col-I), and Collagen type III (Col-III) in kidney sections. (scale bar, 100 µm). ( c ) Quantification of α-SMA-, Col-I-, and Col-III-positive areas (n = 5 in each group). Data are presented as the mean ± SD. ** p
    Recombinant Human Tgf β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human tgf β1/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human tgf β1 - by Bioz Stars, 2021-05
    86/100 stars
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    99
    PeproTech recombinant human tgf β1
    BP reduced collagen I production in lung fibroblast pre-stimulated with recombinant <t>TGF-β1.</t> TGF-β (5 ng/mL) stimulate for 12 h and ( A ) mRNA and ( B ) protein expression levels of BP treatment in several dosages (0, 10, 20, and 30 µg/mL) for 24 h on type I collagen, and SOX2 expressions. ( C ) mRNA and ( D ) protein expression levels of BP treatment for several time points (0, 1, 3, 6, 12, 24, and 48 h) in the dosage of 30 µg/mL on type I collagen and SOX2 expressions. The same amount of DMSO was added in 0 µg/mL groups as a vehicle control. Data showed 3 independent qPCR experiments and presented are mean ± SD. * denotes a significant decrease with the 0 µg/ml group of p
    Recombinant Human Tgf β1, supplied by PeproTech, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human tgf β1/product/PeproTech
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human tgf β1 - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    86
    R&D Systems human tgf β1
    Treatment over control for male and female cells cultured on microstructured Ti surfaces and treated with 17β-estradiol for 24 h at confluence on TCPS. Cell number ( a) and alkaline phosphatase-specific activity in cell lysates was assessed ( b ). Production of osteocalcin ( c ), osteoprotegerin ( d ), and active ( e ) and latent <t>TGF-β1</t> ( f ) after 24-h fresh medium incubation. * p
    Human Tgf β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tgf β1/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tgf β1 - by Bioz Stars, 2021-05
    86/100 stars
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    N/A
    This standard should be reconstituted in 100 µL Milli Q grade water The reconstituted analyte should be used within 60 minutes or aliquoted into screw capped polypropylene vials and stored
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    Image Search Results


    Monocyte chemoattractant protein‐1 mediates tumor growth factor β1‐primed fibrogenic activation of hepatic stellate cells. (A) Experimental approach to analyze the fibrogenic activation of hSCs. LX‐2 cells were treated with TGF‐ß1 for 1 hour and then supplied with fresh medium. MCP‐1 concentration in the culture supernatant was assessed at the indicated times. During cell cultivation, the culture medium was changed daily with or without the presence of MCP‐1 blocking antibody. At the end of the culturing phase, the cell count and type IV collagen protein levels were analyzed. (B) Assessment of extracellular secretion of MCP‐1 in the culture supernatant. Data represent mean ± SD. (C) Cell growth at 120 hours after transient TGF‐β1 stimulation (0, 10 ng/mL and 100 ng/mL) in the presence of either IgG control antibody or MCP‐1 blocking antibody ( # P

    Journal: Hepatology Communications

    Article Title: Role of monocyte chemoattractant protein‐1 in liver fibrosis with transient myeloproliferative disorder in down syndrome

    doi: 10.1002/hep4.1150

    Figure Lengend Snippet: Monocyte chemoattractant protein‐1 mediates tumor growth factor β1‐primed fibrogenic activation of hepatic stellate cells. (A) Experimental approach to analyze the fibrogenic activation of hSCs. LX‐2 cells were treated with TGF‐ß1 for 1 hour and then supplied with fresh medium. MCP‐1 concentration in the culture supernatant was assessed at the indicated times. During cell cultivation, the culture medium was changed daily with or without the presence of MCP‐1 blocking antibody. At the end of the culturing phase, the cell count and type IV collagen protein levels were analyzed. (B) Assessment of extracellular secretion of MCP‐1 in the culture supernatant. Data represent mean ± SD. (C) Cell growth at 120 hours after transient TGF‐β1 stimulation (0, 10 ng/mL and 100 ng/mL) in the presence of either IgG control antibody or MCP‐1 blocking antibody ( # P

    Article Snippet: Cells were stimulated with either recombinant human MCP‐1 (Z028029, GenScript, Piscataway, NJ) or TGF‐β1 (240‐B‐002, R & D Systems, Minneapolis, MN) and then incubated with either 1 μg/mL MCP‐1 blocking antibody (M2420; Sigma, St. Louis, MO) or mouse IgG (278‐810; Ancell, Bayport, MN).

    Techniques: Activation Assay, Concentration Assay, Blocking Assay, Cell Counting

    Anti-fibrotic effects of IFN-γ-treated mesenchymal stem cells (MSCs) in the kidney of IRI rats. IFN-γ-treated or untreated rat MSCs were injected immediately after IRI induction. Twenty-one days later, protein levels of fibrosis markers were evaluated by western blot ( a ) and immunohistochemical ( b , c ) analyses. ( a ) Western blot analysis of α-SMA and TGF-β1 in the rat kidney cortex. Protein levels were normalized to GAPDH levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. ( b ) Representative immunohistochemical staining of α-SMA, Collagen type I (Col-I), and Collagen type III (Col-III) in kidney sections. (scale bar, 100 µm). ( c ) Quantification of α-SMA-, Col-I-, and Col-III-positive areas (n = 5 in each group). Data are presented as the mean ± SD. ** p

    Journal: Scientific Reports

    Article Title: Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis

    doi: 10.1038/s41598-020-79664-6

    Figure Lengend Snippet: Anti-fibrotic effects of IFN-γ-treated mesenchymal stem cells (MSCs) in the kidney of IRI rats. IFN-γ-treated or untreated rat MSCs were injected immediately after IRI induction. Twenty-one days later, protein levels of fibrosis markers were evaluated by western blot ( a ) and immunohistochemical ( b , c ) analyses. ( a ) Western blot analysis of α-SMA and TGF-β1 in the rat kidney cortex. Protein levels were normalized to GAPDH levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. ( b ) Representative immunohistochemical staining of α-SMA, Collagen type I (Col-I), and Collagen type III (Col-III) in kidney sections. (scale bar, 100 µm). ( c ) Quantification of α-SMA-, Col-I-, and Col-III-positive areas (n = 5 in each group). Data are presented as the mean ± SD. ** p

    Article Snippet: Cell treatment with TGF-β1After incubation in DMEM supplemented with 0.1% FBS, CM from control hMSCs, or CM from hMSCs treated with IFN-γ for 24 h, human HK-2 cells were treated with 10 ng/ml recombinant human TGF-β1 (R & D Systems, Minneapolis, MN).

    Techniques: Injection, Western Blot, Immunohistochemistry, Staining

    Inhibitory effects of PTGES siRNA on the anti-fibrotic effects of IFN-γ-treated rMSCs in IRI rats. After rMSCs transfected with NC siRNA or PTGES siRNA were treated with IFN-γ, the cells were injected into rats immediately after IRI induction. Twenty-one days later, protein levels of fibrosis markers in the kidney were evaluated by western blot and immunohistochemical analyses. ( a ) Secreted PGE2 levels in CM from IFN-γ-treated rMSCs transfected with NC siRNA or PTGES siRNA were evaluated by an ELISA (n = 5 in each group). ( b , c ) Western blot analysis of α-SMA and TGF-β1 in the rat kidney cortex of IRI rats. Protein levels were normalized to GAPDH levels (n = 4 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Sham, non-IRI procedure; PBS, PBS injection; NC siRNA, injection of IFN-γ rMSCs transfected with NC siRNA; PTGES siRNA, injection of IFN-γ rMSCs transfected with PTGES siRNA. ( d ) Representative immunohistochemical staining of α-SMA and Col-III in kidney sections (scale bar, 100 µm). ( e ) Quantification of α-SMA- and Col-III-positive areas (n = 4 in each group). Data are presented as the mean ± SD. * p

    Journal: Scientific Reports

    Article Title: Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis

    doi: 10.1038/s41598-020-79664-6

    Figure Lengend Snippet: Inhibitory effects of PTGES siRNA on the anti-fibrotic effects of IFN-γ-treated rMSCs in IRI rats. After rMSCs transfected with NC siRNA or PTGES siRNA were treated with IFN-γ, the cells were injected into rats immediately after IRI induction. Twenty-one days later, protein levels of fibrosis markers in the kidney were evaluated by western blot and immunohistochemical analyses. ( a ) Secreted PGE2 levels in CM from IFN-γ-treated rMSCs transfected with NC siRNA or PTGES siRNA were evaluated by an ELISA (n = 5 in each group). ( b , c ) Western blot analysis of α-SMA and TGF-β1 in the rat kidney cortex of IRI rats. Protein levels were normalized to GAPDH levels (n = 4 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Sham, non-IRI procedure; PBS, PBS injection; NC siRNA, injection of IFN-γ rMSCs transfected with NC siRNA; PTGES siRNA, injection of IFN-γ rMSCs transfected with PTGES siRNA. ( d ) Representative immunohistochemical staining of α-SMA and Col-III in kidney sections (scale bar, 100 µm). ( e ) Quantification of α-SMA- and Col-III-positive areas (n = 4 in each group). Data are presented as the mean ± SD. * p

    Article Snippet: Cell treatment with TGF-β1After incubation in DMEM supplemented with 0.1% FBS, CM from control hMSCs, or CM from hMSCs treated with IFN-γ for 24 h, human HK-2 cells were treated with 10 ng/ml recombinant human TGF-β1 (R & D Systems, Minneapolis, MN).

    Techniques: Transfection, Injection, Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Staining

    DAMPs and the effects of MSCs treated with IFN-γ on renal fibrosis. ( a ) HMGB1 and IL-18 are members of DAMPs—HMGB1 was reported to promote the migration of MSCs, whereas IL-18 contributed to the secretion of IFN-γ. IFN-γ secreted from natural killer cells in injured tissues activates MSCs that exert anti-inflammatory effects. However, such activation require a long period of time, which delays the effect of MSCs administered for therapeutic purposes. ( b ) IFN-γ stimulation promotes the secretion of prostaglandin E2 from MSCs, and increased prostaglandin E2 induces polarization of immunosuppressive CD163-positive macrophages, suppressing the persistence of inflammation. MSCs treated with IFN-γ also directly inhibit profibrogenic TGF-β1/Smad signaling, leading to the prevention of fibrosis.

    Journal: Scientific Reports

    Article Title: Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis

    doi: 10.1038/s41598-020-79664-6

    Figure Lengend Snippet: DAMPs and the effects of MSCs treated with IFN-γ on renal fibrosis. ( a ) HMGB1 and IL-18 are members of DAMPs—HMGB1 was reported to promote the migration of MSCs, whereas IL-18 contributed to the secretion of IFN-γ. IFN-γ secreted from natural killer cells in injured tissues activates MSCs that exert anti-inflammatory effects. However, such activation require a long period of time, which delays the effect of MSCs administered for therapeutic purposes. ( b ) IFN-γ stimulation promotes the secretion of prostaglandin E2 from MSCs, and increased prostaglandin E2 induces polarization of immunosuppressive CD163-positive macrophages, suppressing the persistence of inflammation. MSCs treated with IFN-γ also directly inhibit profibrogenic TGF-β1/Smad signaling, leading to the prevention of fibrosis.

    Article Snippet: Cell treatment with TGF-β1After incubation in DMEM supplemented with 0.1% FBS, CM from control hMSCs, or CM from hMSCs treated with IFN-γ for 24 h, human HK-2 cells were treated with 10 ng/ml recombinant human TGF-β1 (R & D Systems, Minneapolis, MN).

    Techniques: Migration, Activation Assay

    Paracrine effects of IFN-γ-treated human MSCs (hMSCs) on the TGF-β/Smad signaling pathway and phenotypic changes of macrophages. ( a , b ) After HK-2 cells, a human proximal tubular cell line , were incubated with conditioned medium (CM) obtained from IFN-γ-treated or untreated hMSCs for 24 h, the cells were treated with TGF-β1 for 30 min ( a ) or 24 h ( b ). ( a ) Western blot analysis of phosphorylated Smad2 (p-Smad2) and Smad2 in HK-2 cells. p-Smad2 protein levels were normalized to Smad2 levels (n = 5 in each group). ( b ) Western blot analysis of α-SMA in HK-2 cells. α-SMA protein levels were normalized to GAPDH levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Control hMSCs, CM from hMSCs without IFN-γ treatment; IFN-γ hMSCs, CM from hMSCs treated with IFN-γ. ( c ) Concentration of PGE2 in CM from hMSCs was evaluated by an ELISA at 24 or 48 h after IFN-γ stimulation. HK-2 cells were used as a negative control (n = 5 in each group). HK-2, CM from HK-2 cells. ( d ) Phenotypic changes of macrophages were evaluated by CD163 and CD68 protein levels. To induce differentiation of monocytic THP-1 cells into macrophages, the cells were treated with PMA for 48 h. The induced THP-1 cells were incubated with CM from IFN-γ-treated or untreated hMSCs. After 48 h, the cells were harvested and subjected to western blot analysis of CD163, CD206 and CD68. CD163 and CD206 protein levels were normalized to the GAPDH and CD68 levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Data are presented as the mean ± SD. * p

    Journal: Scientific Reports

    Article Title: Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis

    doi: 10.1038/s41598-020-79664-6

    Figure Lengend Snippet: Paracrine effects of IFN-γ-treated human MSCs (hMSCs) on the TGF-β/Smad signaling pathway and phenotypic changes of macrophages. ( a , b ) After HK-2 cells, a human proximal tubular cell line , were incubated with conditioned medium (CM) obtained from IFN-γ-treated or untreated hMSCs for 24 h, the cells were treated with TGF-β1 for 30 min ( a ) or 24 h ( b ). ( a ) Western blot analysis of phosphorylated Smad2 (p-Smad2) and Smad2 in HK-2 cells. p-Smad2 protein levels were normalized to Smad2 levels (n = 5 in each group). ( b ) Western blot analysis of α-SMA in HK-2 cells. α-SMA protein levels were normalized to GAPDH levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Control hMSCs, CM from hMSCs without IFN-γ treatment; IFN-γ hMSCs, CM from hMSCs treated with IFN-γ. ( c ) Concentration of PGE2 in CM from hMSCs was evaluated by an ELISA at 24 or 48 h after IFN-γ stimulation. HK-2 cells were used as a negative control (n = 5 in each group). HK-2, CM from HK-2 cells. ( d ) Phenotypic changes of macrophages were evaluated by CD163 and CD68 protein levels. To induce differentiation of monocytic THP-1 cells into macrophages, the cells were treated with PMA for 48 h. The induced THP-1 cells were incubated with CM from IFN-γ-treated or untreated hMSCs. After 48 h, the cells were harvested and subjected to western blot analysis of CD163, CD206 and CD68. CD163 and CD206 protein levels were normalized to the GAPDH and CD68 levels (n = 5 in each group). The blots are the cropped images from different parts of the same gel. Full-length gel images are provided in the supplementary file. The samples derive from the same experiment and that gels were processed in parallel. Data are presented as the mean ± SD. * p

    Article Snippet: Cell treatment with TGF-β1After incubation in DMEM supplemented with 0.1% FBS, CM from control hMSCs, or CM from hMSCs treated with IFN-γ for 24 h, human HK-2 cells were treated with 10 ng/ml recombinant human TGF-β1 (R & D Systems, Minneapolis, MN).

    Techniques: Incubation, Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control

    BP reduced collagen I production in lung fibroblast pre-stimulated with recombinant TGF-β1. TGF-β (5 ng/mL) stimulate for 12 h and ( A ) mRNA and ( B ) protein expression levels of BP treatment in several dosages (0, 10, 20, and 30 µg/mL) for 24 h on type I collagen, and SOX2 expressions. ( C ) mRNA and ( D ) protein expression levels of BP treatment for several time points (0, 1, 3, 6, 12, 24, and 48 h) in the dosage of 30 µg/mL on type I collagen and SOX2 expressions. The same amount of DMSO was added in 0 µg/mL groups as a vehicle control. Data showed 3 independent qPCR experiments and presented are mean ± SD. * denotes a significant decrease with the 0 µg/ml group of p

    Journal: International Journal of Molecular Sciences

    Article Title: Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide

    doi: 10.3390/ijms19103024

    Figure Lengend Snippet: BP reduced collagen I production in lung fibroblast pre-stimulated with recombinant TGF-β1. TGF-β (5 ng/mL) stimulate for 12 h and ( A ) mRNA and ( B ) protein expression levels of BP treatment in several dosages (0, 10, 20, and 30 µg/mL) for 24 h on type I collagen, and SOX2 expressions. ( C ) mRNA and ( D ) protein expression levels of BP treatment for several time points (0, 1, 3, 6, 12, 24, and 48 h) in the dosage of 30 µg/mL on type I collagen and SOX2 expressions. The same amount of DMSO was added in 0 µg/mL groups as a vehicle control. Data showed 3 independent qPCR experiments and presented are mean ± SD. * denotes a significant decrease with the 0 µg/ml group of p

    Article Snippet: In vitro studies of lung fibrosis were performed in normal Human lung fibroblast (NHLF), which was stimulated into fibrogenesis by recombinant human TGF-β1 (PeproTech, Catalog Number: 100-21, 5 ng/mL) for 12 h.

    Techniques: Recombinant, Expressing, Real-time Polymerase Chain Reaction

    Treatment over control for male and female cells cultured on microstructured Ti surfaces and treated with 17β-estradiol for 24 h at confluence on TCPS. Cell number ( a) and alkaline phosphatase-specific activity in cell lysates was assessed ( b ). Production of osteocalcin ( c ), osteoprotegerin ( d ), and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Journal: Biology of Sex Differences

    Article Title: Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces

    doi: 10.1186/s13293-018-0190-x

    Figure Lengend Snippet: Treatment over control for male and female cells cultured on microstructured Ti surfaces and treated with 17β-estradiol for 24 h at confluence on TCPS. Cell number ( a) and alkaline phosphatase-specific activity in cell lysates was assessed ( b ). Production of osteocalcin ( c ), osteoprotegerin ( d ), and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Article Snippet: Active TGF-β1 was measured prior to acidification of the conditioned media, using an enzyme-linked immunoassay (ELISA) kit specific for human TGF-β1 (TGF-β1 DuoSet, R & D System, Minneapolis, MN).

    Techniques: Cell Culture, Activity Assay, Incubation

    Treatment over control for male and female cells cultured on microstructured Ti surfaces and treated with 1α,25(OH) 2 D 3 for 24 h at confluence on TCPS. Cell number ( a ), and alkaline phosphatase specific activity in cell lysates was assessed ( b ). Production of osteocalcin ( c ), osteoprotegerin ( d ), and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Journal: Biology of Sex Differences

    Article Title: Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces

    doi: 10.1186/s13293-018-0190-x

    Figure Lengend Snippet: Treatment over control for male and female cells cultured on microstructured Ti surfaces and treated with 1α,25(OH) 2 D 3 for 24 h at confluence on TCPS. Cell number ( a ), and alkaline phosphatase specific activity in cell lysates was assessed ( b ). Production of osteocalcin ( c ), osteoprotegerin ( d ), and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Article Snippet: Active TGF-β1 was measured prior to acidification of the conditioned media, using an enzyme-linked immunoassay (ELISA) kit specific for human TGF-β1 (TGF-β1 DuoSet, R & D System, Minneapolis, MN).

    Techniques: Cell Culture, Activity Assay, Incubation

    Donor-specific response of male and female cells cultured on microstructured Ti surfaces and treated with 17β-estradiol for 24 h at confluence on TCPS. Cell number was assessed to measure proliferation ( a , f ). Alkaline phosphatase-specific activity in cell lysates was assessed ( b , g ). Production of osteocalcin ( c , h ), osteoprotegerin ( d , i ), and latent TGF-β1 ( e , j ) after 24-h fresh medium incubation. * p

    Journal: Biology of Sex Differences

    Article Title: Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces

    doi: 10.1186/s13293-018-0190-x

    Figure Lengend Snippet: Donor-specific response of male and female cells cultured on microstructured Ti surfaces and treated with 17β-estradiol for 24 h at confluence on TCPS. Cell number was assessed to measure proliferation ( a , f ). Alkaline phosphatase-specific activity in cell lysates was assessed ( b , g ). Production of osteocalcin ( c , h ), osteoprotegerin ( d , i ), and latent TGF-β1 ( e , j ) after 24-h fresh medium incubation. * p

    Article Snippet: Active TGF-β1 was measured prior to acidification of the conditioned media, using an enzyme-linked immunoassay (ELISA) kit specific for human TGF-β1 (TGF-β1 DuoSet, R & D System, Minneapolis, MN).

    Techniques: Cell Culture, Activity Assay, Incubation

    Response of male and female osteoblasts isolated from human donors to microstructured Ti surfaces (PT, SLA, modSLA). Cell number was assessed to determine proliferation of cells, at confluence on TCPS ( a ). Alkaline phosphatase-specific activity was determined in cell lysates ( b ). Production of osteocalcin ( c) , osteoprotegerin ( d) , and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Journal: Biology of Sex Differences

    Article Title: Human osteoblasts exhibit sexual dimorphism in their response to estrogen on microstructured titanium surfaces

    doi: 10.1186/s13293-018-0190-x

    Figure Lengend Snippet: Response of male and female osteoblasts isolated from human donors to microstructured Ti surfaces (PT, SLA, modSLA). Cell number was assessed to determine proliferation of cells, at confluence on TCPS ( a ). Alkaline phosphatase-specific activity was determined in cell lysates ( b ). Production of osteocalcin ( c) , osteoprotegerin ( d) , and active ( e ) and latent TGF-β1 ( f ) after 24-h fresh medium incubation. * p

    Article Snippet: Active TGF-β1 was measured prior to acidification of the conditioned media, using an enzyme-linked immunoassay (ELISA) kit specific for human TGF-β1 (TGF-β1 DuoSet, R & D System, Minneapolis, MN).

    Techniques: Isolation, Activity Assay, Incubation