OriGene
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OriGene
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Image Search Results

Journal:
Article Title: Binding of LFA-1 (CD11a) to Intercellular Adhesion Molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) Triggers Transmigration of Human Immunodeficiency Virus Type 1-Infected Monocytes through Mucosal Epithelial Cells
doi: 10.1128/JVI.76.1.32-40.2002
Figure Lengend Snippet: Presence of HIV proviral DNA in MDM recovered after transmigration through an epithelial cell monolayer. DNA was extracted from MDM recovered from the basal transwell chamber. The 219-bp amplicon of HIV was visualized after migration on an agarose gel of PCR-amplified proviral DNA. Lane 1, DNA extracted from transmigrated MDM; lane 2, DNA extracted from transmigrated HIV-infected MDM at 4°C; lane 3, DNA extracted from transmigrated HIV-infected MDM at 37°C in the absence of proinflammatory stimuli; lanes 4 to 6, DNA extracted from transmigrated HIV-infected MDM under conditions where HEC-1 cells had been preincubated with anti-CD102 (lane 4) or anti-CD50 (lane 5) or both MAb (lane 6) and the assay was performed under proinflammatory conditions. Results are those from one representative experiment out of three that were performed.
Article Snippet: The following murine monoclonal antibodies (MAb) were purchased from the indicated sources: anti-CD50 (clone HP2/19), anti-CD102 (clone B-T1), anti-CD54 (clone HA58), rhodamine-labeled anti-HIV-1 p24 (RD KC57), Beckman Coulter, Brea, Calif.; anti-CXCR4, anti-CCR5 (clone 2D7), anti-TNF-α, anti-IL-1β, fluorescein-conjugated anti-CD50 (anti-CD50 fluorescein isothiocyanate [FITC]), anti-CD11a FITC (clone H111), anti-CD54 FITC (clone HA58), MAb to human immunoglobulin G (IgG) isotypes, Becton Dickinson, San Diego, Calif.,
Techniques: Transmigration Assay, Amplification, Migration, Agarose Gel Electrophoresis, Infection

Journal:
Article Title: Binding of LFA-1 (CD11a) to Intercellular Adhesion Molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) Triggers Transmigration of Human Immunodeficiency Virus Type 1-Infected Monocytes through Mucosal Epithelial Cells
doi: 10.1128/JVI.76.1.32-40.2002
Figure Lengend Snippet: Expression of CD50 (left), CD102 (middle), and CD54 (right) on the membrane of confluent HEC-1 cells, either unstimulated (top) or stimulated with N. meningitidis LPS (5 ng/ml), IL-1β (2 ng/ml), and TNF-α (10 ng/ml) (bottom). Relevant antibodies were conjugated with FITC, and cells were examined by laser confocal microscopy.
Article Snippet: The following murine monoclonal antibodies (MAb) were purchased from the indicated sources: anti-CD50 (clone HP2/19), anti-CD102 (clone B-T1), anti-CD54 (clone HA58), rhodamine-labeled anti-HIV-1 p24 (RD KC57), Beckman Coulter, Brea, Calif.; anti-CXCR4, anti-CCR5 (clone 2D7), anti-TNF-α, anti-IL-1β, fluorescein-conjugated anti-CD50 (anti-CD50 fluorescein isothiocyanate [FITC]), anti-CD11a FITC (clone H111), anti-CD54 FITC (clone HA58), MAb to human immunoglobulin G (IgG) isotypes, Becton Dickinson, San Diego, Calif.,
Techniques: Expressing, Confocal Microscopy

Journal:
Article Title: Binding of LFA-1 (CD11a) to Intercellular Adhesion Molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) Triggers Transmigration of Human Immunodeficiency Virus Type 1-Infected Monocytes through Mucosal Epithelial Cells
doi: 10.1128/JVI.76.1.32-40.2002
Figure Lengend Snippet: Inhibition of transmigration by preincubation of HEC-1 cells with anti-CD50, anti-CD54, and anti-CD102 MAb. The HEC-1 monolayer was preincubated with anti-CD50, anti-CD54, or anti-CD102 MAb (50 μg/ml) or with a mixture of anti-CD50 and anti-CD102 MAb for 30 min at room temperature prior to performing the transmigration assay under proinflammatory conditions. The results are expressed as in Fig. Fig.44.
Article Snippet: The following murine monoclonal antibodies (MAb) were purchased from the indicated sources: anti-CD50 (clone HP2/19), anti-CD102 (clone B-T1), anti-CD54 (clone HA58), rhodamine-labeled anti-HIV-1 p24 (RD KC57), Beckman Coulter, Brea, Calif.; anti-CXCR4, anti-CCR5 (clone 2D7), anti-TNF-α, anti-IL-1β, fluorescein-conjugated anti-CD50 (anti-CD50 fluorescein isothiocyanate [FITC]), anti-CD11a FITC (clone H111), anti-CD54 FITC (clone HA58), MAb to human immunoglobulin G (IgG) isotypes, Becton Dickinson, San Diego, Calif.,
Techniques: Inhibition, Transmigration Assay