Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Resistin Enhances Monocyte Chemoattractant Protein-1 Production in Human Synovial Fibroblasts and Facilitates Monocyte Migration.

doi: 10.33594/000000029

Figure Lengend Snippet: Fig. 5. Resistin promotes MCP-1-mediated monocyte migration by inhibiting miR-33a and miR-33b. (A&B) OASFs were incubated with resistin for 24 h and miRNA expression was examined by qPCR. (C) OASFs were transfected with the miR-33a and miR-33b mimics then incubated with resistin for 24 h; MCP-1 expression was measured by qPCR. (D) CM was applied to THP-1 cells and analyzed for migration activity. Results are expressed as the mean ± SEM. *p<0.05 as compared with the control group; #p<0.05 as compared with the resistin-treated group.

Article Snippet: KG Chen et al.: Resistin Enhances MCP-1-Mediated Monocyte Migration Materials and Methods Materials Recombinant human resistin was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Migration, Incubation, Expressing, Transfection, Activity Assay, Control

Journal: Journal of biomedical science

Article Title: Neutrophil-derived reactive agents induce a transient SpeB negative phenotype in Streptococcus pyogenes.

doi: 10.1186/s12929-023-00947-x

Figure Lengend Snippet: Fig. 1 Reversible loss of SpeB is associated with tissue pathology and inflammation. A Distribution of SpeB+ and SpeB− GAS clones directly isolated from NSTI patient tissue biopsies (n = 23). B Percentage of SpeB+ and SpeB− GAS clones after the passage in THY media (p1, passage 1; p2, passage 2). Representative analysis of 2006 GAS patient isolate is shown. C–G Correlation analysis of bacterial load (left panel; n = 81 biopsies) or percentage of SpeB− clones (right panel; n = 23 biopsies) with the presence of HMGB1 (C), IL-8 (D), infiltrating neutrophils (E), and resistin (F) in patient biopsies. Correlation was determined using Spearman test. Semiquantitative acquired computerized image analyses (ACIA) of immuno-histochemical staining were performed as described in the methods section. The cell area was defined by the hematoxylin counterstaining, and the results are presented as percent positively stained area × mean intensity of positive staining

Article Snippet: The following antibodies were used for immunohistochemistry: anti-human HMGB1 (clone EPR3507; Abcam), anti-human IL-8 (clone NAP-1; Invitrogen), anti-human resistin (clone 184,305; R&D systems), and anti-human neutrophilelastase (clone NP57; DAKO).

Techniques: Clone Assay, Isolation, Staining

Journal: The Journal of Clinical Hypertension

Article Title: Masked Hypertension and Atherogenesis: The Impact on Adiponectin and Resistin Plasma Levels

doi: 10.1111/j.1751-7176.2008.00070.x

Figure Lengend Snippet: Resistin and Adiponectin Plasma Levels of the Study Groups

Article Snippet: The adiponectin plasma levels (RD195023100; Human Adiponectin ELISA Bio Vendor Laboratory Medicine Inc, Brno, Czech Republic) and the resistin plasma levels (RD191016100; Human Resistin ELISA Bio Vendor Laboratory Medicine Inc.) were measured by immunoassay .

Techniques: Clinical Proteomics

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: Resistin induces HCT-116 and SW-48 cell adhesion to HUVECs. HCT-116 and SW-48 cells were maintained as controls or stimulated with resistin and then stained with a crystal violet solution. The adhesion of HCT-116 and SW-48 cells to HUVECs was assessed. HCT-116 and SW-48 cells incubated with resistin (50 ng/mL) plus resistin-neutralizing antibodies (Ab) exhibited no increase in HUVEC adhesion. Data represent the mean ± standard error of the mean (SEM) from four independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. cells treated with resistin only.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Staining, Incubation, Control

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: Resistin increases the expressions of ICAM-1 and VCAM-1 in HCT-116 cells. HCT-116 cells were maintained as control or stimulated with resistin. ICAM-1 and VCAM-1 mRNA ( A , B ) and protein ( C , D ) expression was analyzed. Data in ( A , B ) represent the mean ± SEM from three independent experiments. The results in ( C , D ) are representative of three independent experiments with similar results. * p < 0.05 vs. control cells.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: Blocking ICAM-1 and VCAM-1 in HCT-116 cells inhibits their adhesion to HUVECs. HCT-116 cells were pretreated with specific neutralizing antibodies ( A ) or siRNAs ( B ) for control (IgG/si-CL), ICAM-1, VCAM-1, or both and then were maintained as control or stimulated with resistin. HCT-116 cell adhesion was determined. Data represent the mean ± SEM from four independent experiments. * p < 0.05 vs. control cells (CL); # p < 0.05 vs. cells pretreated with IgG or si-CL and then treated with resistin only; ** p < 0.05 vs. cells pretreated with ICAM-1 or VCAM-1 neutralizing antibody and then treated with resistin.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Blocking Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: Resistin-initiated HCT-116 adhesion to HUVECs is mediated by NF-κB activation. ( A ) and ( C ) HCT-116 cells were maintained as control or stimulated with resistin for 1, 2, and 4 h and then NF-κB activation ( A ) and p65 phosphorylation ( C ) were determined by ELISA kit and Western blot, respectively; ( B , D , E ) HCT-116 cells were pretreated with ( B , D ) DMSO or NF-κB inhibitors (PDTC or SN50) and ( E ) control or p65 specific siRNA and then stimulated with resistin. HCT-116 cell adhesion and ICAM-1/VCAM-1 mRNA expression were analyzed. Data represent the mean ± SEM from three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. DMSO or si-CL cells with resistin treatment; § p < 0.05 vs. PDTC, SN50, or si-p65 cells without resistin treatment.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Activation Assay, Control, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: FA inhibits resistin-initiated HCT-116 cell adhesion to HUVECs. ( A ) HCT-116 cells were pretreated with FA at 0, 1, 5 and 10 μg/mL and then were maintained as control or stimulated with resistin; ( B ) HCT-116 cells were pretreated with FA at 0 or 10 μg/mL and then were maintained as control or stimulated with resistin at 10, 25 and 50 ng/mL. HCT-116 cell adhesion was determined. Data represent the mean ± SEM from three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. cells treated with resistin only.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: FA inhibits resistin-induced ICAM-1 and VCAM-1 expression in HCT-116 cells. HCT-116 cells were pretreated with FA at 0, 1, 5 and 10 μg/mL and then were maintained as control or stimulated with resistin. ICAM-1 and VCAM-1 mRNA ( A , B ) and protein ( C , D ) expression was determined. Data in ( A , B ) represent the mean ± SEM from three independent experiments. The results in ( C , D ) are representative of three independent experiments with similar results. * p < 0.05 vs. control cells; # p < 0.05 vs. cells treated with resistin only.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Fulvic Acid Attenuates Resistin-Induced Adhesion of HCT-116 Colorectal Cancer Cells to Endothelial Cells

doi: 10.3390/ijms161226174

Figure Lengend Snippet: FA inhibits the resistin effect on HCT-116 cell adhesion to HUVECs by down-regulating NF-κB activity. HCT-116 cells were pretreated with FA at 0, 1, 5 and 10 μg/mL and then were maintained as control or stimulated with resistin. The activation of NF-κB was determined. Data represent the mean ± SEM from three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. cells treated with resistin only.

Article Snippet: Recombinant human resistin was purchased from R & D Systems (Minneapolis, MN, USA).

Techniques: Activity Assay, Control, Activation Assay

Journal: Cell Reports Medicine

Article Title: Clinical-proteomic classification and precision treatment strategy of chordoma

doi: 10.1016/j.xcrm.2024.101757

Figure Lengend Snippet: Imageological-proteomic analysis of chordoma (A) Representative CT manifestation of sclerotic and osteolytic chordoma. Protein levels (B) and Representative IHC images (C) of RETN in chordoma with or without bone destruction. GSEA enrichment analysis of DEPs between chordoma with or without bone destruction revealed statistic difference in degradation of the ECM (D) and osteopontin signaling (E). (F) Representative T1-weighted images of MRI for chordoma (hypointensity, isointensity, heterogeneous hyperintensity). (G) Kaplan-Meier curve of OS between hypo-/iso-intensity and heterogeneous hyperintensity. Protein levels (H) and representative IHC images (I) of AHNAK2 in chordoma hypo-/iso-intensity or heterogeneous hyperintensity. GSEA enrichment analysis of DEPs between hypo-/iso-intensity and heterogeneous hyperintensity revealed statistic difference in mTORC1 signaling (J) and G2M DNA damage checkpoint (K). Abbreviations: CT, computed tomography; IHC, immunohistochemistry; OS, overall survival; DEPs, differentially expressed proteins; GSEA, gene set enrichment analysis; ECM, extracellular matrix; MRI, magnetic resonance imaging.

Article Snippet: RETN Polyclonal antibody , Proteintech , Cat# 18170-1-AP; RRID: AB_2918058.

Techniques: Computed Tomography, Immunohistochemistry, Magnetic Resonance Imaging

Journal: Cell Reports Medicine

Article Title: Clinical-proteomic classification and precision treatment strategy of chordoma

doi: 10.1016/j.xcrm.2024.101757

Figure Lengend Snippet:

Article Snippet: RETN Polyclonal antibody , Proteintech , Cat# 18170-1-AP; RRID: AB_2918058.

Techniques: Diagnostic Assay, Recombinant, Sequencing, Software