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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Effects of CoCl 2 -simulated hypoxia on the expression levels of matrix metalloproteinases in renal adenocarcinoma cells and renal tubular epithelial cells
doi: 10.3892/etm.2018.6337
Figure Lengend Snippet: (A-F) Effects of chemical hypoxia on the expression levels of MMPs differed. (A, C and E) Expression levels of RECK, MMP2, MMP9 and MMP14 in HK2, 786-0 and HMEC-1 cells were determined by western blot analysis after incubation with varying concentrations of CoCl2 from 0 to 200 µmol/l for 24 h. (B, D and F) Normalized densitometric quantitation. *P<0.05, #P>0.05 as indicated. In all cases, at least three independent experiments were performed. MMP, matrix metalloproteinase; HK2, human renal tubular epithelial cells; 786-0, human renal cell cancer cells; HMEC-1, human microvascular endothelial cells.
Article Snippet: The source and dilution of unconjugated antibodies used in this study are as follows: HIF-1α (rabbit pAb; NB100-134SS, 1:1,000; Novus Biologicals, Ltd., Cambridge, UK),
Techniques: Expressing, Western Blot, Incubation, Quantitation Assay