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Image Search Results

Journal: Oncology Letters
Article Title: miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1
doi: 10.3892/ol.2017.5709
Figure Lengend Snippet: miR-150 is downregulated in OS patient tissues and cell lines. (A) Relative expression levels of miR-150 in 40 OS patient tissue samples and adjacent normal tissue sample counterparts. The relative expression levels of miR-150 were determined using RT-qPCR analysis. (B) Relative expression levels of miR-150, determined using RT-qPCR analysis in U2OS, MG63 and SaOS2 OS cell lines and the normal hFOB1.19 human OS cell line. Student's t -test was used to analyze significant differences.*P<0.05. Data are presented as the mean + standard deviation. OS, osteosarcoma; miR, microRNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
Article Snippet: The SaOS2, U2OS and
Techniques: Expressing, Quantitative RT-PCR, Standard Deviation, Real-time Polymerase Chain Reaction

Journal: Oncology Letters
Article Title: miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1
doi: 10.3892/ol.2017.5709
Figure Lengend Snippet: miR-150 overexpression is inversely correlated with OS cell growth. (A) U2OS, (B) MG63 and (C) SaOS2 OS cell lines were transfected with miR-150 and the numbers of cells were determined at different time points using a fluorescence-based CyQUANT cell proliferation assay kit. In all cases, Student's t -test was used to analyze significant differences, *P<0.05. Data are presented as the mean + standard deviation. OS, osteosarcoma; miR, microRNA; NC, negative control.
Article Snippet: The SaOS2, U2OS and
Techniques: Over Expression, Transfection, Fluorescence, CyQUANT Assay, Proliferation Assay, Standard Deviation, Negative Control

Journal: Oncology Letters
Article Title: miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1
doi: 10.3892/ol.2017.5709
Figure Lengend Snippet: miR-150 overexpression is inversely correlated with OS cell migration and invasion. Transwell migration and invasion assays of (A) U2OS, (B) MG63 and (C) SaOS2 OS cell lines. The assays were performed following transfection of the cell lines with miR-150. Representative images of migrated and invaded cells on the membrane (magnification, ×100). OS, osteosarcoma; miR, microRNA; NC, negative control.
Article Snippet: The SaOS2, U2OS and
Techniques: Over Expression, Migration, Transfection, Negative Control

Journal: Oncology Letters
Article Title: miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1
doi: 10.3892/ol.2017.5709
Figure Lengend Snippet: miR-150 negatively regulates gene expression of ROCK1 in OS cell lines. (A) Reverse transcription-quantitative polymerase chain reaction analysis of mRNA expression of ROCK1 in U2OS, MG63 and SaOS2 OS cell lines overexpressing miR150. Student's t -test was used to analyze significant differences. *P<0.05. Data are presented as the mean + standard deviation. (B) Western blot analysis of protein expression of ROCK1 in U2OS, MG63 and SaOS2 OS cell lines overexpressing miR150. A corresponding NC was used in the absence of miR-150. β-actin protein was used as an additional control. miR, microRNA; ROCK1, Rho-associated kinase 1; NC, negative control.
Article Snippet: The SaOS2, U2OS and
Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, Western Blot, Negative Control