Journal: Cell Stem Cell

Article Title: Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells

doi: 10.1016/j.stem.2022.08.001

Figure Lengend Snippet:

Article Snippet: StemMACSTM Klf4 mRNA, human , Miltenyi Biotech , Cat#130-101-115.

Techniques: Recombinant, Transfection, Protease Inhibitor, Generated, In Vitro, Isolation, Software, Microscopy

Journal: Frontiers in Endocrinology

Article Title: Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial

doi: 10.3389/fendo.2022.841971

Figure Lengend Snippet: Baseline sample characteristics, the DietBra Trial.

Article Snippet: Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKineTM ELISA Kit and Human IL-6 PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions.

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial

doi: 10.3389/fendo.2022.841971

Figure Lengend Snippet: Comparisons of inflammatory biomarkers at the end of the intervention between two intervention groups. (A) TNF-α; (B) IL-1α; (C) MCP-1; (D) adiponectin; (E) IL-6; (F) leptin. Differences in TNF-α, MCP-1 adiponectin IL-6 and leptin were analyzed by the Mann-Whitney test and the difference in IL-lα was analyzed by the independent t-test. * p <0.05.

Article Snippet: Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKineTM ELISA Kit and Human IL-6 PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions.

Techniques: MANN-WHITNEY

Journal: Frontiers in Endocrinology

Article Title: Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial

doi: 10.3389/fendo.2022.841971

Figure Lengend Snippet: Comparison between baseline and final follow-up variables in two intervention groups with class II/III obesity, Brazil, 2016 (n = 40).

Article Snippet: Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKineTM ELISA Kit and Human IL-6 PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions.

Techniques: Comparison, Fat, Marker, Synthesized

Journal: Frontiers in Endocrinology

Article Title: Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial

doi: 10.3389/fendo.2022.841971

Figure Lengend Snippet: Comparison of changes (delta) in outcome variables between two intervention groups with class II/III obesity, Brazil (n = 34).

Article Snippet: Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKineTM ELISA Kit and Human IL-6 PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions.

Techniques: Comparison, Marker, Synthesized

Journal: Frontiers in Endocrinology

Article Title: Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial

doi: 10.3389/fendo.2022.841971

Figure Lengend Snippet: Comparison of outcome variables at the end of intervention between two intervention groups with class II/III obesity, Brazil, 2016 (n = 34).

Article Snippet: Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKineTM ELISA Kit and Human IL-6 PicoKineTM ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions.

Techniques: Comparison, Marker, Synthesized

Journal: BioMed Research International

Article Title: Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells

doi: 10.1155/2018/5241941

Figure Lengend Snippet: Primer sequences and Tm for RT-qPCR.

Article Snippet: The primary antibodies included B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X (Bax), IRE-1, FAS associated via death domain (FADD), FAS, β -actin (Wuhan Boster Biological Technology, Ltd.,), TNF- α , CHOP and caspase-12 (BIOSS, Beijing, China) at 1:500 dilution and Cleaved-caspase3 (Cell Signaling Technology, Inc., Danvers, MA, USA), cleaved-caspase8 (Beyotime Institute of Biotechnology, Shanghai, China) at 1:1 000 dilution.

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Vascular Protective Effects of Malus toringoides (Rehd.) Hughes Extracts and their Mechanism in Diabetic Rats and HUVECs

doi: 10.1155/2022/4348435

Figure Lengend Snippet: Effects of EMT on CX3CL1 expression in rat aortic histological sections and HUVECs. (a) Immunohistochemical detection of CX3CL1 expression in aortic sections from a diabetic rat model established for 6 weeks and administered treatment for 8 weeks. (b, c) HUVECs were pretreated for 12 h with the indicated concentrations of EMT and stimulated for 48 h with high glucose (25 mM). Western blot and ELISA analysis of CX3CL1 and CX3CR1 were performed as described in the materials and methods section. (d) After high glucose treatment, intense immunoreactions were observed in CX3CL1-positive cells (green), and blue staining of the nuclei was observed by DAPI. The values are mean ± SD of 3 independent experiments with triplicate samples. # P < 0.05 or ## P < 0.01 compared to the group treated with high glucose.

Article Snippet: ELISA kits for C-reactive protein (CRP, EK0978) tumor necrosis factor alpha (TNF- α , EK0526), interleukin 1 β (IL-1 β , EK0393), IL-6 (EK0412), VCAM-1 (EK0537), ICAM-1 (EK0370), E-selectin (EK0501) and CX3CL1 (EK0356) were obtained from BOSTER Biological Technology (Wuhan, China).

Techniques: Expressing, Immunohistochemical staining, Western Blot, Enzyme-linked Immunosorbent Assay, Staining