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Image Search Results

Journal: Molecules
Article Title: Exopolysaccharide from Lactobacillus plantarum HY7714 Protects against Skin Aging through Skin–Gut Axis Communication
doi: 10.3390/molecules26061651
Figure Lengend Snippet: Effect of HY7714 EPS on ECM modulation in HS68 cells. ( A ) The intracellular level of matrix metalloproteinase1 (MMP1) in HS68 cells treated with HY7714 EPS was measured using an MMP1 ELISA kit. ( B , C ) Relative mRNA levels of MMP1 and HAS1 in HS68 cells were calculated by qPCR and normalized against GAPDH. ( D ) Western blotting for MMP1 and COLa1 in HS68 cells, and quantification of band density. Data are expressed as means ± SD ( n = 4), and values labeled with different letters are significantly different, p < 0.05 (a > b > c). CON, control cells.
Article Snippet: Cell culture medium was collected, and MMP1 was quantified using commercial
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Labeling

Journal: Molecules
Article Title: Exopolysaccharide from Lactobacillus plantarum HY7714 Protects against Skin Aging through Skin–Gut Axis Communication
doi: 10.3390/molecules26061651
Figure Lengend Snippet: Effect of HY7714 EPS on protein levels of MMPs and intracellular hyaluronic acid (HA) in UVB-irradiated HS68 cells. ( A ) Relative levels of intracellular MMP1 in HS68 cells treated with HY7714 EPS were measured using an MMP1 ELISA kit. MMP1 production was amplified by pre-treatment with 100 ng/mL TNFα for 2 h. ( B ) Intracellular HA in UVB-exposed cells treated with HY7714 EPS was measured using an HA ELISA kit. ( C ) Western blotting for MMP1 and MMP13 in HS68 cells, and quantification of band density. Data are expressed as means ± SD ( n = 3), and values labeled with different letters are significantly different, p < 0.05 (a > b > c).
Article Snippet: Cell culture medium was collected, and MMP1 was quantified using commercial
Techniques: Irradiation, Enzyme-linked Immunosorbent Assay, Amplification, Western Blot, Labeling

Journal: Stem Cell Research & Therapy
Article Title: Exosomes derived from human umbilical cord mesenchymal stem cells inhibit vein graft intimal hyperplasia and accelerate reendothelialization by enhancing endothelial function
doi: 10.1186/s13287-020-01639-1
Figure Lengend Snippet: hucMSC-exosomes inhibited intimal hyperplasia and luminal stenosis in arterialized vein grafts. a Representative ultrasound images of vein grafts from the PBS group (left panel) and exosome group (right panel). Quantification of the luminal diameter ( b ) and peak-systolic velocity ( c ) in vein grafts. d Histologic images of haematoxylin and eosin staining (HE) staining in vein grafts from the normal vein group (left panel), PBS group (middle panel), and exosome group (right panel). e Quantification of the neointimal thickness in vein grafts. f Immunohistochemical staining of matrix metalloproteinase 2 (MMP-2) in vein grafts from the PBS group (left panel) and exosome group (right panel). g Immunohistochemical staining of matrix metalloproteinase 9 (MMP-9) in vein grafts from the PBS group (left panel) and exosome group (right panel). h Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in vein grafts from the PBS group (left panel) and exosome group (right panel). i Quantitative analysis of PCNA-positive index in vein grafts. The PCNA-positive index was quantified as the percentage of total nuclei in the neointima. Scale bar is 200 μm in panels d , f , g , and h . The results are presented as the mean ± SD, n = 6 for each group. An asterisk represents statistically significant difference compared with the PBS group ( P < 0.05). Number sign represents statistically significant difference compared with the normal vein group ( P < 0.05)
Article Snippet:
Techniques: Staining, Immunohistochemical staining