Journal: BMC gastroenterology

Article Title: The impact of probiotic supplementation on gastric motility and nutrient absorption in elderly patients with Gastrointestinal disorders.

doi: 10.1186/s12876-025-03740-2

Figure Lengend Snippet: Fig. 3 Comparison of intestinal permeability markers levels between two groups (after treatment 7d). (A) Occludin; (B) Zonulin; (C) LBP. LBP: Lipopolysac charide Binding Protein. *: P < 0.05; **: P < 0.01.Paired t-tests were used to compare pre- vs. post-treatment values within each group; independent t-tests were used for between-group comparisons at day 7

Article Snippet: The specific ELISA kits employed included: the human zonulin kit (EKC 36091, Biomatik USA, LLC; Wilmington, DE, USA), the human occludin kit (NBP2-80305, Novus Biologicals, LLC; Centennial, CO, USA), and the human LBP kit (DY870-05, R&D Systems, Inc.; Minneapolis, MN, USA).

Techniques: Comparison, Permeability, Binding Assay

Journal: Frontiers in Immunology

Article Title: Leukotriene A 4 Hydrolase Is a Candidate Predictive Biomarker for Successful Allergen Immunotherapy

doi: 10.3389/fimmu.2020.559746

Figure Lengend Snippet: Serum proteins with significant change of expression levels after 1-year treatment.

Article Snippet: Leukotriene A 4 hydrolase (LTA 4 H) (abx572445, Abbexa Ltd, Cambridge, UK), Mucin 5 subtype B (MUC5B) (CSB-E11201h; CUSABIO, Hubei, China), lipopolysaccharide binding protein (LBP) (DY870-05, R&D Systems Inc) and C4b-binding protein (C4BPB) (CSB-EL003954HU; CUSABIO, Hubei, China) from serum samples were measured using ELISA according to the manufacturer’s protocols.

Techniques: Expressing, Molecular Weight, Sequencing, Activation Assay

Journal: Frontiers in Immunology

Article Title: Leukotriene A 4 Hydrolase Is a Candidate Predictive Biomarker for Successful Allergen Immunotherapy

doi: 10.3389/fimmu.2020.559746

Figure Lengend Snippet: Validation of proteomic prognostic biomarkers using enzyme-linked immunosorbent assay (ELISA). The serum level of LTA 4 H in allergen immunotherapy (AIT) non-responders (A) and AIT responders (B) . (C) Receiver-operator characteristic (ROC) curve of LTA 4 H measured using ELISA. The serum level of MUC5B in AIT non-responders (D) and responders (E) . The serum level of lipopolysaccharide binding protein (LBP) in AIT non-responders (F) and responders (G) . The serum level of C4BPB in AIT non-responders (H) and responders (I) .

Article Snippet: Leukotriene A 4 hydrolase (LTA 4 H) (abx572445, Abbexa Ltd, Cambridge, UK), Mucin 5 subtype B (MUC5B) (CSB-E11201h; CUSABIO, Hubei, China), lipopolysaccharide binding protein (LBP) (DY870-05, R&D Systems Inc) and C4b-binding protein (C4BPB) (CSB-EL003954HU; CUSABIO, Hubei, China) from serum samples were measured using ELISA according to the manufacturer’s protocols.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Cell genomics

Article Title: Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe.

doi: 10.1016/j.xgen.2022.100248

Figure Lengend Snippet: Figure 5. Functional impact of negatively selected variants (A–D) Functional study of the LBP D283G variant. (A) qRT-PCR for LBP on cDNA from HEK293T cells non-transfected (NT) or transfected with an empty plasmid (EV), or plasmids encoding ancestral or derived LBP. Dots indicate three independent experiments, and the height of each bar their mean values. (B) Western blot of whole-cell lysates or cell culture supernatants from HEK293T cells either left NT, transfected with an EV, or C-terminally tagged plasmids expressing ancestral or derived LBP forms. LBP was detected with a polyclonal anti-LBP antibody and an antibody against the C-terminal DDK tag. An antibody against GADPH was used as loading control. The results shown are representative of three independent experiments. (C) LBP concentration in cell culture supernatants from transfected HEK293T cells as measured by ELISA. Dots indicate three independent experiments, and the height of each bar their mean values. (D) Binding of ancestral or derived LBP collected from cell culture supernatant at increasing concentrations to LPS and Pam2CSK4 assessed by a binding assay. Each point represents the mean of two biological replicates ± SD. (E and F) Functional study of the IL23R R381Q variant. (E) T cell blasts from seven healthy controls homozygous for the ancestral variant, seven patients ho- mozygous for the derived IL23R R381Q variant, and an IL-12Rb1-deficient patient were left unstimulated or were stimulated with IL-23 (ranging from 1 to 100 ng/ mL) or IFN-a2b as a positive control. STAT3 phosphorylation was evaluated by flow cytometry. A Wilcoxon test was used to assess whether observed control and IL23R R381Q-derived values belong to the same population. *p < 0.05, **p < 0.01. (F) T cell blasts from nine healthy controls homozygous for the ancestral IL23R variant, two individuals homozygous for IL23R R381Q, two homozygous for TYK2 P1104A, one TYK2/ patient, and one IL-12Rb1/ patient were stimulated with IL-1b for 24 h and with IL-12 or IL-23 for 6 h. Then, RNA sequencing was performed. The impact of the IL23R R381Q on the IFN-g network was dissected by performing gene set enrichment analysis (GSEA: fgsea) using hallmark gene sets (http://www.gsea-msigdb.org/) and a mean log2 fold-change (log2FC) in each condition. Dot heatmaps are shown for the 10 most strongly affected gene sets in healthy controls on IL-23 plus IL-1b stimulation when compared with IL-1b stimulation. (G–I) Functional study of the TLR3 L412F variant. (G) Principal-component analysis (PCA) of RNA sequencing-quantified gene expression for two controls ho- mozygous for the ancestral TLR3 variant, four TLR3 L412F homozygotes, and one complete TLR3-deficient primary fibroblast cell left non-stimulated (NS) or

Article Snippet: Membranes were probed by incubation for 1 h at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592, Merck, 1:10,000) and GAPDH (#sc-47724, Santa Cruz Biotechnology, 1:5,000).

Techniques: Functional Assay, Variant Assay, Quantitative RT-PCR, Transfection, Plasmid Preparation, Derivative Assay, Western Blot, Cell Culture, Expressing, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Positive Control, Phospho-proteomics, Cytometry, RNA Sequencing, Gene Expression

Journal: Cell Genomics

Article Title: Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe

doi: 10.1016/j.xgen.2022.100248

Figure Lengend Snippet:

Article Snippet: Site-directed mutagenesis was performed on a pCMV6 plasmid (#RC221961, OriGene) containing the LBP ancestral sequence, with appropriate primers ( ) and the Pfu Ultra II Fusion HS DNA (#600674, Agilent) polymerase, followed by digestion with Dpn I (#R0176L, New England Biolabs).

Techniques: Produced, Virus, Recombinant, Transfection, SYBR Green Assay, Western Blot, Protease Inhibitor, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Staining, Control, Software, Sequencing, DNA Library Preparation