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Amersham Life Sciences Inc
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Image Search Results
Journal: Retrovirology
Article Title: Identification and characterization of naturally occurring splice variants of SAMHD1
doi: 10.1186/1742-4690-9-86
Figure Lengend Snippet: Immunofluorescence analysis of SAMHD1 WT and splice variants. HeLa cells were either mock transfected (panel A ) or transfected with 1 μg of pcDNA-SAMHD1 WT (panel B ), 5 μg of Δ14 (panel C ), or 4 μg of Δ8-9 (panel D ). Total amounts of transfected DNA were adjusted to 5 μg with empty vector DNA as necessary. After the transfection, cells were replated onto cover slips, grown for 24 h, and then fixed with methanol (10 min, -20°C). Cells were dually stained with rabbit polyclonal antibody to SAMHD1 (SAM416; red) and a mouse monoclonal antibody to lamin-B (green). Samples were analyzed on a confocal microscope as detailed in Methods.
Article Snippet: A mouse monoclonal antibody to
Techniques: Immunofluorescence, Transfection, Plasmid Preparation, Staining, Microscopy