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Image Search Results
![Percentage survival was monitored using the xCELLigence RTCA of cetuximab-sensitive (Cet Sen ) cell lines ( a ), acquired cetuximab-resistant (Acq Res ) ( b ) and intrinsically cetuximab-resistant (Int Res ) cell lines which were either HPV-negative ( c ) or HPV-positive ( d ), until 48 h after treatment. Tumour cells were either treated with an isotype control (IgG1κ) or cetuximab [2 µg/ml], with or without healthy donor-derived NK cells (effector to target (E:T) ratio of 5:1) under both normoxic and hypoxic conditions. Fraction of ADCC induced killing was obtained by subtraction of the means of cetuximab and NK cells from cetuximab alone condition ( e ). Effect of hypoxia was determined within the different cetuximab resistance groups ( f ) and HPV status ( g ) within each group. Graphs represent mean ± SEM for each time point of follow up. Statistical significance was tested using a linear mixed model with Tukey’s pairwise comparison. NS not significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2851/pmc07462851/pmc07462851__41416_2020_934_Fig3_HTML.jpg)
Journal: British Journal of Cancer
Article Title: Cetuximab-induced natural killer cell cytotoxicity in head and neck squamous cell carcinoma cell lines: investigation of the role of cetuximab sensitivity and HPV status
doi: 10.1038/s41416-020-0934-3
Figure Lengend Snippet: Percentage survival was monitored using the xCELLigence RTCA of cetuximab-sensitive (Cet Sen ) cell lines ( a ), acquired cetuximab-resistant (Acq Res ) ( b ) and intrinsically cetuximab-resistant (Int Res ) cell lines which were either HPV-negative ( c ) or HPV-positive ( d ), until 48 h after treatment. Tumour cells were either treated with an isotype control (IgG1κ) or cetuximab [2 µg/ml], with or without healthy donor-derived NK cells (effector to target (E:T) ratio of 5:1) under both normoxic and hypoxic conditions. Fraction of ADCC induced killing was obtained by subtraction of the means of cetuximab and NK cells from cetuximab alone condition ( e ). Effect of hypoxia was determined within the different cetuximab resistance groups ( f ) and HPV status ( g ) within each group. Graphs represent mean ± SEM for each time point of follow up. Statistical significance was tested using a linear mixed model with Tukey’s pairwise comparison. NS not significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.
Article Snippet: After overnight incubation, cetuximab [2 µg/ml] or
Techniques: Derivative Assay