Journal: Developmental cell

Article Title: TRAIL Receptors Serve as Stress-Associated Molecular Patterns to Promote ER-Stress-Induced Inflammation.

doi: 10.1016/j.devcel.2020.01.031

Figure Lengend Snippet: Figure 2. Taxane-Induced Cytokine Production Is FADD and TRAIL Receptor Dependent (A) HeLa cells were treated with taxol in the presence or absence of z-VAD-fmk or q-VD-OPh (10 mM). After 24 h, apoptosis was determined by morphological assessment on triplicate counts of a minimum of 100 cells per field and cytokines in culture supernatants were determined by ELISA.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ficoll-Paque Plus GE Life Sciences Cat #17-1440-02 Recombinant human M-CSF Medical Supply Co. Ltd Cat #213-10280–1 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma Chemical Co. Cat #I6758 Imidazole Sigma Chemical Co. Cat #I5513 Kanamycin Sigma Chemical Co. Cat #60615 Ampicillin Sigma Chemical Co. Cat #A9518 Pfu DNA Polymerase Promega Cat #M774A DpnI enzyme New England BioLabs Cat #R0176L Fast Digest BsmBI New England BioLabs Cat #B7024 T4 polynucleotide kinase New England BioLabs Cat #M0201 T4 ligation buffer New England BioLabs Cat #B0202S Quick ligase New England BioLabs Cat #M2200 Fast alkaline phosphatase Thermofisher Scientific Cat #EF0654 GenJet transfection reagent SignaGen Cat #SL100488 Blasticidin S hydrochloride Sigma Chemical Co. Cat #15205 Puromycin dihydrochloride Sigma Chemical Co. Cat #P9620 Propidium iodide (PI) Sigma Chemical Co. Cat #P4170 Triton-X100 Sigma Chemical Co. Cat #T8532 Strepavidin-sepharose High Performance GE Life Sciences Cat #17-5113-01 Protein A/G-PLUS-Agarose Santa-Cruz Biotechnology Cat #sc-2003 Amintra NiNTA Resin Expedeon Cat #ANN0025 GeneJet SignaGen Laboratories Cat #SL100488 SuperKiller TRAIL Adipogen Cat #AG-40T-0002-C020 Recombinant IL-1-a Martin Laboratory N/A Recombinant TNF-a Roche Cat #11371843001 Flow cytometry staining buffer Invitrogen Cat #00-4222-26 SlowFade Gold antifade mountant Invitrogen Cat #S36936 Critical Commercial Assays Human IL-6 ELISA Duoset R&D systems Cat #DY206 Human IL-8 ELISA Duoset R&D systems Cat #DY208 Human CXCL1 ELISA Duoset R&D systems Cat #DY275 Human RANTES ELISA Duoset R&D systems Cat #DY278 Human MCP-1 ELISA Duoset R&D systems Cat #DY279 Human GM-CSF ELISA Duoset R&D systems Cat #DY215 Human IL-3 ELISA Duoset R&D systems Cat #DY203 Human Eotaxin ELISA Duoset R&D systems Cat #DY320 Mouse CXCL1/KC ELISA Duoset R&D systems Cat #DY453 Mouse CCL2 ELISA Duoset R&D systems Cat #DY479 Mouse RANTES ELISA Duoset R&D systems Cat #DY478 RNEasy Mini Kit Qiagen Cat #74104 QuantiTect RT Kit Qiagen Cat #205313 QuantiFast SYBR Green RT-PCR Kit Qiagen Cat #205414 Deposited Data Raw and analyzed data This paper N/A Experimental Models: Cell Lines Human: HeLa Martin Laboratory Cullen et al., 2013 Human: HeLa SEAP Martin Laboratory Henry et al., 2016 Human: HeLa CHOP -/- Martin Laboratory N/A Human: HeLa FADD -/- Martin Laboratory N/A (Continued on next page) Developmental Cell 52, 1–17.e1–e5, March 23, 2020 e2

Techniques: Enzyme-linked Immunosorbent Assay