human il 10 Search Results


anti il10 apc antibodies  (Miltenyi Biotec)
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Miltenyi Biotec anti il10 apc antibodies
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human il 10 quantikine elisa kits  (R&D Systems)
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R&D Systems human il 10 quantikine elisa kits
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human il 10  (R&D Systems)
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R&D Systems human il 10
Human Il 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 10 quantikine immunoenzymatic kit  (R&D Systems)
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Human Il 10 Quantikine Immunoenzymatic Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd4 t cells  (R&D Systems)
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R&D Systems human cd4 t cells
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rhil 10  (R&D Systems)
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R&D Systems rhil 10
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il 10  (Elabscience Biotechnology)
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Elabscience Biotechnology il 10
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hil  (R&D Systems)
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R&D Systems hil
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goat anti human il10 antibodies  (R&D Systems)
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R&D Systems goat anti human il10 antibodies
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
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anti il 10 receptor alpha antibodies  (R&D Systems)
93
R&D Systems anti il 10 receptor alpha antibodies
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Anti Il 10 Receptor Alpha Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human elisa kit  (Diaclone)
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Diaclone human elisa kit
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Human Elisa Kit, supplied by Diaclone, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

Journal: Human gene therapy

Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.

doi: 10.1089/hum.2010.175

Figure Lengend Snippet: FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

Article Snippet: After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA).

Techniques: In Vitro, Expressing, Plasmid Preparation, Cloning, Virus, Transduction, Western Blot, Marker