Journal: Neural Plasticity
Article Title: Neural Stem Cell Transplant-Induced Effect on Neurogenesis and Cognition in Alzheimer Tg2576 Mice Is Inhibited by Concomitant Treatment with Amyloid-Lowering or Cholinergic α7 Nicotinic Receptor Drugs
Figure Lengend Snippet: Treatment with (+)-phenserine increases the survival of transplanted hNSCs in the dentate gyrus of Tg2576 mice. (a) The total number of cells labeled with anti-human nuclei (hNuclei) and with 4′,6-diamidino-2-phenylindole (DAPI) nuclear counterstain following transplantation of hNSCs into the dentate gyrus (DG) in Tg2576 mice. (b) The proportion of cells showing immunoreactivity for microtubule-associated protein 2 (MAP2) or glial fibrillary acidic protein (GFAP), normalized to the total number of hNuclei+ cells (%) in the DG of Tg2576 mice. (c-d) Representative images of cells stained with hNuclei (red) and with MAP2 (green) in the DG of hNSC-transplanted mice treated with (c) saline (hNSC + SAL) or (d) (+)-phenserine (hNSC + PHEN) at 20x magnification. (e-f) Representative images of cells stained with hNuclei (red) and with GFAP (green) in the DG of hNSC-transplanted mice treated with (e) saline or (f) (+)-phenserine at 20x magnification. Cell nuclei were visualized with DAPI. # p
Article Snippet: Immunohistochemistry For staining of differentiated hNSCs in culture, cells were incubated with rabbit anti-human glial fibrillary acidic protein (GFAP, Dako, Glostrup, Denmark), mouse anti-β III-tubulin (Sigma, St. Louis, MO, USA), or mouse polyclonal anti-microtubule-associated protein 2 (MAP2, Sigma, St. Louis, MO, USA).
Techniques: Mouse Assay, Labeling, Transplantation Assay, Staining