Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/elife.52709

Figure Lengend Snippet: Figure 6. Phytate-mediated Ca2+ deficiency promotes vitamin D insufficiency and renal phosphate wasting independent of FGF23 expression. (A–C) Time-course analysis of serum levels of intact PTH (A), 25(OH)D (B), and 1,25(OH)2D (C) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. For the early measurement of 25(OH)D (B) and 1,25(OH)2D, we pooled the sera from 2 to 3 rats. (D–F) Time-course analysis of renal CYP27B1 (D), CYP24A1 (E), and VDR (F) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. (G) Immunoblot analysis of renal aKlotho, NHERF1, NaPi-2a in rats fed control, HP-LCa2+, Figure 6 continued on next page

Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Rattus norvegicus) Sprague Dawley rat Orient Bio, Seoul, Korea Developed by Sprague Dawley, Inc. Chemical compound, drug AIN-93G Dyets Inc. DYET# 10700 Control diet Chemical compound, drug Phytic acid Sigma-Aldrich Cat. #: P-8810 Phytate diet Chemical compound, drug HNO3 Sigma-Aldrich Cat. #: 438073 Fecal mineral analysis Chemical compound, drug HF Sigma-Aldrich Cat. #: 695068 Fecal mineral analysis Chemical compound, drug HCl Sigma-Aldrich Cat. #: H1758 Fecal mineral analysis Chemical compound, drug EDTA Sigma-Aldrich Cat. #: 93283 Fecal phytate analysis Chemical compound, drug NaOH Sigma-Aldrich Cat. #: 415413 Fecal phytate analysis Chemical compound, drug Calcium Beckman Coulter OSR6113 Serum/urine biochemistry Chemical compound, drug Phosphate Beckman Coulter OSR6122 Serum/urine biochemistry Chemical compound, drug BUN (Urea) Beckman Coulter OSR6134 Serum/urine biochemistry Chemical compound, drug Creatinine Beckman Coulter OSR6178 Serum/urine biochemistry Chemical compound, drug Magnesium Beckman Coulter OSR6189 Serum/urine biochemistry Chemical compound, drug Urine protein Beckman Coulter OSR6170 Urine biochemistry Commercial assay or kit Rat intact PTH Immutopics Cat. #: 60–2500 ELISA kit Commercial assay or kit Human PTH Abcam Cat. #: ab230931 ELISA kit Commercial assay or kit Rat soluble RANKL Immundiagnostik Cat. #: K1019 ELISA kit Commercial assay or kit Rat osteoprotegerin Alpco Immunoassay Cat. #: 30–1020 ELISA kit Commercial assay or kit Rat intact FGF23 Elabscience Cat. #: E-EL-R3031 ELISA kit Commercial assay or kit Rat C-terminal FGF23 Elabscience Cat. #: E-EL-RB0377 ELISA kit Commercial assay or kit Human FGF23 Elabscience Cat. #: E-EL-H1116 ELISA kit Commercial assay or kit 25(OH)-Vitamin D Abbott/USA ARCHITECT 25-OH Vitamin D Chemiluminescence microparticle immunoassay Commercial assay or kit 1,25-(OH)two vitamin D DIAsoure 1,25(OH)2-VIT, D-RIA-CT/Belgium Radioimmunoassay Continued on next page Kim et al. eLife 2020;9:e52709.

Techniques: Expressing, Control, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Transcriptional profiling reveals roles of intercellular Fgf9 signaling in astrocyte maturation and synaptic refinement during brainstem development

doi: 10.1016/j.jbc.2022.102176

Figure Lengend Snippet: Top 20 differentially expressed genes per cell cluster

Article Snippet: Bcan (1:1000, catalog no.: 610894; BD Biosciences) and Hapln1 (1:400, catalog no.: AF2604; R&D Systems) antibodies were used to label the PNNs.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Transcriptional profiling reveals roles of intercellular Fgf9 signaling in astrocyte maturation and synaptic refinement during brainstem development

doi: 10.1016/j.jbc.2022.102176

Figure Lengend Snippet: Fgf9 cKO mice have increased Bcan volume in the PNN. A and B , Bcan staining ( red ) is increased in Fgf9 cKO mice at P14 compared to WT littermates and displays a staining pattern that is less interdigitated. C and D , Hapln1 staining ( magenta ) appears similar between WT and KO mice. The scale bars represent 10 μm. E , quantification of Bcan volume surrounding the principal neurons reveals a significant increase in Fgf9 cKO mice (mean volume of WT = 4216 ± 529.4 μm 3 and mean volume of cKO = 4986 ± 510.3 μm 3 , p ≤ 0.05, n = 6, unpaired t test). F , volumetric analysis shows no significant change in Hapln1 volumes between genotypes (mean volume of WT = 4330 ± 1192.4 μm 3 and mean volume of cKO = 4952 ± 905.4 μm 3 , p ≥ 0.05, n = 5, unpaired t test). Each point on the graph represents the average of all quantified Bcan- or Hapln1-containing nets/replicate. Sixteen nets were quantified/replicate. Error bars represent SD. cKO, conditional KO; PNN, perineuronal net.

Article Snippet: Bcan (1:1000, catalog no.: 610894; BD Biosciences) and Hapln1 (1:400, catalog no.: AF2604; R&D Systems) antibodies were used to label the PNNs.

Techniques: Staining

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: Interaction of Klotho-EGFP N614Q with the proteasome and ER-chaperones. ( A ) Scheme of the GFP-TRAP immunoprecipitation followed by mass spectroscopy (MS). HEK239T cells expressing GFP or Klotho-EGFP variants were used. Created with BioRender.com. ( B ) Bar graph illustrating KEGG pathway analysis of proteins exhibiting increased interaction with Klotho-EGFP N614Q compared to Klotho-EGFP WT . ( C ) Heatmap plot of proteins that exhibit enhanced interaction with Klotho-EGFP N614Q associated with protein processing in the ER . The color code indicates a log2-fold change. ( D ) Western blot analysis of proteins co-IPed with Klotho-EGFP variants from lysates of HEK293T cells stably expressing Klotho-EGFP WT or N614Q after GFP-TRAP IP. Samples were probed with antibodies against Klotho, HSPA5, and ERGIC53. Where indicated, cells were treated with 2.5 nM FGF23 for 5 min. ( E ) Quantification of the interaction of Klotho-EGFP variants with HSPA5 and ERGIC53; displayed is the relative intensity of HSPA5 or ERGIC53 to KL in %. (n = 4 experiments, mean ± SD, two-sided Student’s t -test. For full-size blots, see .

Article Snippet: Dulbecco’s modified Eagle Medium + GlutaMax (Gibco, Waltham, MS, USA, 61965-026), Hygromycin B (50 mg/mL) (Thermo Fischer Scientific, 10687010, Waltham, MS, USA), penicillin/streptomycin (Merck, P0781, Rahway, NJ, USA), Fetal Bovine Serum (F7524 Sigma-Aldrich, Burlington, MA), ROTI ® Histofix 4% paraformaldehyde (Carl Roth, No. P087.1, Karlsruhe, Germany), Hoechst 33342 (Invitrogen, H1399, Waltham, MS, USA), Human FGF23 protein (R&D Systems, 2604, Minneapolis, MN, USA), LipofectamineTM 2000 Transfection Reagent (Thermo Fischer Scientific, 11668019), Protease Inhibitor Cocktail (Sigma P8340, Burlington, MA, USA), phosphatase inhibitors (PhosSTOP, Roche, #4906837001, Basel, Switzerland), CHAPSO (Carl Roth), I-BlockTM powder (T2015, Applied Biosystems, Thermo Fisher Scientific).

Techniques: Immunoprecipitation, Mass Spectrometry, Expressing, Western Blot, Stable Transfection

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: N-glycosylation is not essential for Klotho-FGFR interaction. HEK293T cells stably expressing Klotho-EGFP variants were serum-starved for 16 h and incubated for 5 min with FGF23, followed by lysis and processing for Western blotting with the indicated antibodies. ( B ) Quantification of n = 4 independent experiments, as shown in ( A ). The ratio of the phospho (p)-ERK to total (t)-ERK in the presence and absence of FGF23 is displayed as mean ± SD, with statistical analysis performed using a two-sided Student’s t -test (ns: non-significant) Vertical black lines indicate splicing; for full-size blots, see .

Article Snippet: Dulbecco’s modified Eagle Medium + GlutaMax (Gibco, Waltham, MS, USA, 61965-026), Hygromycin B (50 mg/mL) (Thermo Fischer Scientific, 10687010, Waltham, MS, USA), penicillin/streptomycin (Merck, P0781, Rahway, NJ, USA), Fetal Bovine Serum (F7524 Sigma-Aldrich, Burlington, MA), ROTI ® Histofix 4% paraformaldehyde (Carl Roth, No. P087.1, Karlsruhe, Germany), Hoechst 33342 (Invitrogen, H1399, Waltham, MS, USA), Human FGF23 protein (R&D Systems, 2604, Minneapolis, MN, USA), LipofectamineTM 2000 Transfection Reagent (Thermo Fischer Scientific, 11668019), Protease Inhibitor Cocktail (Sigma P8340, Burlington, MA, USA), phosphatase inhibitors (PhosSTOP, Roche, #4906837001, Basel, Switzerland), CHAPSO (Carl Roth), I-BlockTM powder (T2015, Applied Biosystems, Thermo Fisher Scientific).

Techniques: Glycoproteomics, Stable Transfection, Expressing, Incubation, Lysis, Western Blot

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: FGF23 does not activate ER-resident Klotho-EGFP-EGFR3 complexes. ( A , C ) Western blot of HEK293T cell lysates stably expressing Klotho-EGFP WT or N614Q after treatment with different FGF23 concentrations for 5 min ( A ) or with 2.5 nM FGF23 for the indicated times ( B ), probed with the indicated antibodies. p, phospho;t, total. ( B ) Quantification of n = 4 independent experiments from ( A ). Displayed is the ratio of phospho(p)-ERK normalized to total (t)-ERK. ( C ) A representative blot from n = 2 independent experiments. ( D ) HEK293T cells stably expressing EGFP, Klotho-EGFP WT, or N614Q-treated with 2.5 nM FGF23 for 5 min were subjected to IP with GFP-TRAP. IP lysates (Inp., input) after IP were separated by SDS-PAGE, blotted, and probed with antibodies against FGFR3 or Klotho. ( E ) Quantification of n = 3 independent experiments from ( D ). Displayed is the ratio of FGFR3 to Klotho-EGFP variant. A two-sided Student’s t -test was used to assess statistical significance * p < 0.05. Error bars indicate SD. Vertical black lines indicate splicing; for full-size blots, see .

Article Snippet: Dulbecco’s modified Eagle Medium + GlutaMax (Gibco, Waltham, MS, USA, 61965-026), Hygromycin B (50 mg/mL) (Thermo Fischer Scientific, 10687010, Waltham, MS, USA), penicillin/streptomycin (Merck, P0781, Rahway, NJ, USA), Fetal Bovine Serum (F7524 Sigma-Aldrich, Burlington, MA), ROTI ® Histofix 4% paraformaldehyde (Carl Roth, No. P087.1, Karlsruhe, Germany), Hoechst 33342 (Invitrogen, H1399, Waltham, MS, USA), Human FGF23 protein (R&D Systems, 2604, Minneapolis, MN, USA), LipofectamineTM 2000 Transfection Reagent (Thermo Fischer Scientific, 11668019), Protease Inhibitor Cocktail (Sigma P8340, Burlington, MA, USA), phosphatase inhibitors (PhosSTOP, Roche, #4906837001, Basel, Switzerland), CHAPSO (Carl Roth), I-BlockTM powder (T2015, Applied Biosystems, Thermo Fisher Scientific).

Techniques: Western Blot, Stable Transfection, Expressing, SDS Page, Variant Assay

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: Low amounts of Klotho-EGFP at the plasma membrane are sufficient for ERK activation. ( A ) HEK293T cells stably expressing Klotho-EGFP at low (l), median (m), and high (h) levels were incubated with 2.5 nM FGF23 for 5 min, lysed, separated on SDS-PAGE, and subjected to Western Blotting with the indicated antibodies. ( B ) Quantification of n = 4 independent experiments from A. The displayed ratio is p-ERK/t-ERK. A Two-sided Student’s t -test was used to assess statistical significance, (ns: non-significant). Error bars indicate SD. For full-size blots, see .

Article Snippet: Dulbecco’s modified Eagle Medium + GlutaMax (Gibco, Waltham, MS, USA, 61965-026), Hygromycin B (50 mg/mL) (Thermo Fischer Scientific, 10687010, Waltham, MS, USA), penicillin/streptomycin (Merck, P0781, Rahway, NJ, USA), Fetal Bovine Serum (F7524 Sigma-Aldrich, Burlington, MA), ROTI ® Histofix 4% paraformaldehyde (Carl Roth, No. P087.1, Karlsruhe, Germany), Hoechst 33342 (Invitrogen, H1399, Waltham, MS, USA), Human FGF23 protein (R&D Systems, 2604, Minneapolis, MN, USA), LipofectamineTM 2000 Transfection Reagent (Thermo Fischer Scientific, 11668019), Protease Inhibitor Cocktail (Sigma P8340, Burlington, MA, USA), phosphatase inhibitors (PhosSTOP, Roche, #4906837001, Basel, Switzerland), CHAPSO (Carl Roth), I-BlockTM powder (T2015, Applied Biosystems, Thermo Fisher Scientific).

Techniques: Clinical Proteomics, Membrane, Activation Assay, Stable Transfection, Expressing, Incubation, SDS Page, Western Blot

Journal: Cells

Article Title: Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein Klotho

doi: 10.3390/cells13201743

Figure Lengend Snippet: ( A ) Venn diagram illustrating the overlap and distinctions between differentially up-regulated phosphorylated proteins in Klotho-EGFP WT and N614Q mutant after FGF23 treatment. ( B ) Pathway enrichment analysis depicting the differentially up-regulated phosphorylated proteins between Klotho-EGFP WT and N614Q after FGF23 treatment, highlighting the activation of key signaling pathways. ( C , D ) Heatmap illustrating the differentially expressed phosphosites and the predicted kinases responsible for their phosphorylation (the rows) in cells expressing Klotho-EGFP WT ( C ) or Klotho-EGFP N614Q ( D ). The red arrows on the right side of each heatmap indicate the presence of the ERK downstream target ELK1 and MAPK1 itself. The arrows at the bottom of the heatmap denote the predicted MAPKs responsible for phosphorylating the phosphosites depicted in the heatmap.

Article Snippet: Dulbecco’s modified Eagle Medium + GlutaMax (Gibco, Waltham, MS, USA, 61965-026), Hygromycin B (50 mg/mL) (Thermo Fischer Scientific, 10687010, Waltham, MS, USA), penicillin/streptomycin (Merck, P0781, Rahway, NJ, USA), Fetal Bovine Serum (F7524 Sigma-Aldrich, Burlington, MA), ROTI ® Histofix 4% paraformaldehyde (Carl Roth, No. P087.1, Karlsruhe, Germany), Hoechst 33342 (Invitrogen, H1399, Waltham, MS, USA), Human FGF23 protein (R&D Systems, 2604, Minneapolis, MN, USA), LipofectamineTM 2000 Transfection Reagent (Thermo Fischer Scientific, 11668019), Protease Inhibitor Cocktail (Sigma P8340, Burlington, MA, USA), phosphatase inhibitors (PhosSTOP, Roche, #4906837001, Basel, Switzerland), CHAPSO (Carl Roth), I-BlockTM powder (T2015, Applied Biosystems, Thermo Fisher Scientific).

Techniques: Mutagenesis, Activation Assay, Protein-Protein interactions, Phospho-proteomics, Expressing

Journal: Frontiers in Nephrology

Article Title: Role of kidney transplantation in long-term cardiac reverse remodeling and interconnecting mechanisms in type 4 cardiorenal syndrome

doi: 10.3389/fneph.2024.1455036

Figure Lengend Snippet: (A) displays the distribution of post-KT FGF23 levels based on the hypertension status. (B) shows that the patients with larger LA volumes had the worst NHYA class.

Article Snippet: FGF23 levels were quantified using the Human FGF23 monoclonal antibody and R&D Systems reagent (Minneapolis, MN, USA).

Techniques:

Journal: Frontiers in Nephrology

Article Title: Role of kidney transplantation in long-term cardiac reverse remodeling and interconnecting mechanisms in type 4 cardiorenal syndrome

doi: 10.3389/fneph.2024.1455036

Figure Lengend Snippet: Biochemical and echocardiographic characteristics grouped according the basal LVEF and after KT.

Article Snippet: FGF23 levels were quantified using the Human FGF23 monoclonal antibody and R&D Systems reagent (Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23

doi: 10.1371/journal.pone.0104154

Figure Lengend Snippet: (A) Gross appearance, tail length, and body weight. Compared with control mice, Fgfr1 Dmp1-cKO -null mice had normal gross appearance and body weight. However, Hyp mice showed considerably shorter tail length and lower body weight, compound Hyp ; Fgfr1 Dmp1-cKO -null mice displayed intermediate tail length and body weight between control and Hyp mice. Data are mean ± S.D. from 5–6 individual mice. (B and C) Western blot analysis of total Fgf2 and Fgf23 protein levels in bone. A representative Fgf2, Fgf23, and β-Actin gel were shown in upper, middle, and lower panels of B, respectively. The intensity of bands was quantified using Image J software ( http://rsb.info.nih.gov/ij/ ), and the data shown in C are mean ± S.D. from three independent experiments. Values sharing the same superscript in different groups are not significantly different at P <0.05.

Article Snippet: Antibody against Fgf23 (MAB2629) was obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques: Control, Western Blot, Software

Journal: PLoS ONE

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23

doi: 10.1371/journal.pone.0104154

Figure Lengend Snippet: Gene-expression profiles in bone in 6-week-old mice.

Article Snippet: Antibody against Fgf23 (MAB2629) was obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques:

Journal: PLoS ONE

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23

doi: 10.1371/journal.pone.0104154

Figure Lengend Snippet: Biochemistry analysis in 6-week-old mice.

Article Snippet: Antibody against Fgf23 (MAB2629) was obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques:

Journal: PLoS ONE

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23

doi: 10.1371/journal.pone.0104154

Figure Lengend Snippet: MC3T3-E1 cells were co-transfected with various plasmids and treated with different drugs as described in Material and Methods. (A) Dose-dependent stimulation of mouse FGF23 promoter activity by recombinant FGF2 (5∼100 ng/ml); (B) Dominate-negative FGFR1(TK−) constructs blocked the stimulatory effect of recombinant FGF2 (50 ng/ml) on mouse FGF23 promoter activity; (C) Effect of PLCγ inhibitor (U73122) on FGF2-induced FGF23 promoter activity; (D) Effect of PI3K inhibitor Wortmannin (Wort) on FGF2-induced FGF23 promoter activity; (E) Dose-dependent inhibition of FGF2-induced FGF23 promoter activation by MAPK inhibitors (U0126). (F) Overexpression of HMW- Fgf2 constructs stimulated mouse FGF23 promoter activity. Data are expressed as the mean ± S.D. from triple three independent experiments. Values sharing the same superscript in different groups are not significantly different at P <0.05. * indicates significant difference from control vector group.

Article Snippet: Antibody against Fgf23 (MAB2629) was obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques: Transfection, Activity Assay, Recombinant, Construct, Inhibition, Activation Assay, Over Expression, Control, Plasmid Preparation

Journal: PLoS ONE

Article Title: Osteocyte-Specific Deletion of Fgfr1 Suppresses FGF23

doi: 10.1371/journal.pone.0104154

Figure Lengend Snippet: MC3T3-E1 cells were co-transfected with pcDNA3.1- FGFR1 expression plasmids and human pcDNA3.1-FGF23-V5-His expression plasmids and treated with different drugs as described in Material and Methods. (A and B) Time-dependent stimulation of human FGF23-V5 protein expression by recombinant FGF2 (50 ng/ml); (C and D) Effect of PI3K inhibitor Wortmannin (Wort) and protein synthesis inhibitor Cycloheximide (CXM) on FGF2-induced human FGF23-V5 protein expression. Both Wortmannin and Cycloheximide completely blocked FGF2-induced FGF23 protein increase. Data are expressed as the mean ± S.D. from triple three independent experiments. Values sharing the same superscript in different groups are not significantly different at P <0.05.

Article Snippet: Antibody against Fgf23 (MAB2629) was obtained from R&D Systems, Inc. (Minneapolis, MN).

Techniques: Transfection, Expressing, Recombinant

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Dependence of the SPRi signal on FGF23 antibody concentration. The used concentration of the FGF23-αKlotho complex was 40:169.2 pg/mL; pH = 7.4. “Standard deviation (SD)” was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Dependence of the SPRi signal on the FGF23 concentration. The used FGF23 antibody concentration is 25 ng/mL; pH = 7.4. SD was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Dependence of the SPRi signal on the FGF23 concentration with the addition of αKlotho protein. The used FGF23 antibody concentration is 25 ng/mL; pH = 7.4. SD was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Influence of the αKlotho protein on the detected FGF23 concentration. SD was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Influence of the time on the formation of the FGF23-αKlotho complex. SD was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: The influence of the potential interferents on the determination of the FGF23 with array SPRi biosensor. SD was calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Precision and accuracy of the FGF23 SPRi biosensor. SD and RSD were calculated from 36 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Precision and recovery of the FGF23 SPRi biosensor tested in a plasma sample after the addition of a 25 pg/mL spike. SD and RSD were calculated from 12 individual measurements.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Clinical Proteomics

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Formation of the FGF23 SPRi biosensor layers, i.e., bare gold, cysteamine, FGF23 antibody, and FGF23-αKlotho complex, captured via SEM. Used parameters: 12.5 or 15 kV, and 100,000× magnification.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: The comparison of the results of FGF23 concentration measurements performed with the SPRi biosensor and ELISA in blood plasma samples. SD was calculated based on 12 measurements for the SPRi biosensor and 2 for the ELISA.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: Comparison, Concentration Assay, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: International Journal of Molecular Sciences

Article Title: A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

doi: 10.3390/ijms242015327

Figure Lengend Snippet: Scheme of the measuring system of the SPRi apparatus and an overview diagram of the FGF23 biosensor.

Article Snippet: The monoclonal mouse anti-human FGF23 antibody (R&D Systems) was a receptor that bound the FGF23 protein from samples to the chip’s surface.

Techniques: