Journal: PLoS ONE

Article Title: Cellular Effects of HER3-Specific Affibody Molecules

doi: 10.1371/journal.pone.0040023

Figure Lengend Snippet: Binding of Alexa Fluor® 488-labelled Affibody molecules (150 nM) to: A. MCF-7, B. SKBR-3 and the HER3-negative cell line SKOV-3. “Bl” = blocking with 15 μM of the corresponding, non-labelled Affibody. MCF-7 cells were stained in a separate experiment, whereas SKBR-3 and SKOV-3 were stained simultaneously.

Article Snippet: As a positive control, a polyclonal goat anti-human HER3 antibody (AF234, R&D systems) was used.

Techniques: Binding Assay, Blocking Assay, Staining

Journal: PLoS ONE

Article Title: Cellular Effects of HER3-Specific Affibody Molecules

doi: 10.1371/journal.pone.0040023

Figure Lengend Snippet: Images showing AU565, SKBR-3, MCF-7 and SKOV-3 cells stained with HER3-specific Affibody molecules Z05416 and Z05417, respectively. The polyclonal anti-HER3 antibody A234 and ZTaq were used as positive and negative staining controls, respectively. Affibody molecules and antibodies binding to cells are shown in green while nuclear staining by DAPI is given in blue. AU565 and SKOV-3 images were acquired on the same day using the same detection gain and laser power, enabling comparison between staining intensities. Cell staining of MCF-7 and SKBR-3 was analysed on different days, using different detection gains for optimal image acquisition. Additionally, MCF-7 images were acquired using increased laser power.

Article Snippet: As a positive control, a polyclonal goat anti-human HER3 antibody (AF234, R&D systems) was used.

Techniques: Staining, Negative Staining, Binding Assay, Comparison

Journal: PLoS ONE

Article Title: Cellular Effects of HER3-Specific Affibody Molecules

doi: 10.1371/journal.pone.0040023

Figure Lengend Snippet: Histograms showing ELISA absorbance results for detection of: A. Phospho-HER2 in MCF-7 cells, B. Phospho-HER3 in MCF-7 cells, C. Phospho-HER2 in SKBR-3 cells and D. Phospho-HER3 in SKBR-3 cells. Cells were incubated without HRG (grey bars) or with 0.05 nM HRG (black bars), in combination with the Affibody molecules (100 nM) before being lysed and analysed in an ELISA.

Article Snippet: As a positive control, a polyclonal goat anti-human HER3 antibody (AF234, R&D systems) was used.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation