Journal: Antioxidants (Basel, Switzerland)

Article Title: Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening.

doi: 10.3390/antiox12010051

Figure Lengend Snippet: Figure 1. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human keratinocyte HaCaT cells. (A) HaCaT cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HaCaT cells were irradiated with UVB (30 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HaCaT cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (30 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HaCaT cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Article Snippet: The Type I Pro-Collagen amount was determined using a Human Pro-Collagen I α1 ELISA kit from R&D Systems (Minneapolis, MN, USA).

Techniques: Irradiation, MTT Assay, Western Blot, Reverse Transcription, Enzyme-linked Immunosorbent Assay

Journal: Antioxidants (Basel, Switzerland)

Article Title: Skin Improvement with Antioxidant Effect of Yuja ( Citrus junos ) Peel Fractions: Wrinkles, Moisturizing, and Whitening.

doi: 10.3390/antiox12010051

Figure Lengend Snippet: Figure 2. Anti-wrinkle effects of YJP-EA, Hex, and BuOH on UVB-irradiated human dermal fibroblast cells. (A) HDF cells were treated with YJP-EA, Hex, and BuOH for 24 h. An MTT assay was performed to evaluate cell viability. (B) HDF cells were irradiated with UVB (100 mJ/cm2) and then treated with YJP-EA, Hex, and BuOH for 24 h. Whole cell lysates were analyzed by Western blot analysis. (C) HDF cells were treated by YJP-EA, Hex, and BuOH for 24 h after UVB (100 mJ/cm2) irradiation. The RNA level was evaluated using reverse transcription PCR. (D) The Pro-Collagen of HDF cells was measured using an ELISA kit, following the manufacturer’s instructions, with 450 nm. All experiments were performed individually in triplicate. *** p < 0.001 vs. non-treated (NT) cells, ** p < 0.01 vs. non-treated (NT) cells, and * p < 0.05 vs. non-treated (NT) cells.

Article Snippet: The Type I Pro-Collagen amount was determined using a Human Pro-Collagen I α1 ELISA kit from R&D Systems (Minneapolis, MN, USA).

Techniques: Irradiation, MTT Assay, Western Blot, Reverse Transcription, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: In Vitro and Clinical Evaluation of the Anti-Wrinkle Efficacy of Medipep-6PN, a Novel Peptide Identified by Phage Display

doi: 10.3390/ijms27041753

Figure Lengend Snippet: In vitro evaluation of the effects of Medipep-6PN on MMP-1 activity, MMP-1 expression, and collagen I synthesis. ( a ) Analysis of the inhibitory effect of Medipep-6PN on MMP-1 activity using a fluorescence assay performed in technical triplicate. The inhibition rate is expressed as a percentage compared to the untreated control group, and 1,10-phenanthroline monohydrate served as a positive control. ( b ) Evaluation of the effects of Medipep-6PN on MMP-1 mRNA expression in HDFs induced by UVB (10 mJ/cm 2 ) irradiation, performed in technical triplicate. Gray bars (non-irradiated normal control group), green bars (UVB only), blue bars (Medipep-6PN-treated group), and light blue bars (TGF-β1-treated group) are the positive control. ( c ) The results of the stimulation of collagen I production by Medipep-6PN in HDFs, performed in technical triplicate. The production rate is expressed as a percentage compared to the untreated control group, and statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test. ** p < 0.01 vs. the untreated control (CTL) group; ns, not significant. ( d ) Immunofluorescence images demonstrating the effects of Medipep-6PN on collagen I production in HDFs. First column: DAPI (blue); second column: procollagen Iα1 (green); third column: merged images of nuclei and procollagen I.

Article Snippet: The Human procollagen Iα1 DuoSet ® ELISA kit (DY6220-05, R&D Systems, Minneapolis, MN, USA) was used, and the evaluation was performed according to the manufacturer’s provided experimental protocol.

Techniques: In Vitro, Activity Assay, Expressing, Fluorescence, Inhibition, Control, Positive Control, Irradiation, Immunofluorescence

Journal: bioRxiv

Article Title: Microsecond pulse electrical stimulation modulates cell migration

doi: 10.1101/2022.10.23.513372

Figure Lengend Snippet: (a) A graphical illustration showed the effects of μsPEF (pulse width:20 μs, frequency: 10 Hz, duration: 5 s) on the skin wound, promoting cell migration and extracellular matrix remodeling. (b) Representative time-lapse images showing the fibroblasts morphology after μsPEF (i.e., 750 and 1500 V/cm) treatment of different intensity at different time points (i.e., 0, 1 and 2 h). Detached cells are marked by yellow arrow. Scale bar, 50 μm. (c) The line graph representing the cell migration average speed per 1 h from 0 to 12 h after different intensity μsPEF (i.e., 750 and 1500 V/cm) treatment (blue circle: control; orange square: 750 V/cm; pink triangle: 1500 V/cm). Results are presented as mean ± standard deviation with 95% CI (n CTRL =42 cells, n 750 v/cm =48 cells, n 1500 v/cm =47 cells); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (d) Box plot showing the average cell migration speed with different intensity μsPEF (i.e., 750 and 1500 V/cm) and control treatments in 24 h. Results are presented as mean ± standard deviation with 95% CI (n CTRL =470 cells, n 750 V/cm =979 cells, n 1500 V/cm =535 cells). (e) Effects of μsPEF on the secretion of COLA2. The level of collagen type I α2 in cellular supernatants was measured after 48 h. The concentration of COLA2 was 0.109 ± 0.018 ng/mL in the control group, 0.257 ± 0.058 ng/mL in the 750 V/cm group and 0.363 ± 0.034 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=5); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (f) Effects of μsPEF on the expression of FGF2. The level of FGF2 in cellular supernatants was measured after 48 h. The concentration of FGF2 was 44.139 ± 0.360 ng/mL in the control group, 48.012 ± 1.488 ng/mL in the 750 V/cm group and 48.523 ± 1.944 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=3); ns=0.7329, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons.

Article Snippet: Then, the cells were cultured in serum-free medium for 48 h. The content of type I α collagen and basic fibroblast growth factor (FGF-2) in the supernatant were measured using commercially available Human COL1A2 (Collagen Type I Alpha 2) ELISA Kit (Elabscience, Wuhan, China) and Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit (Elabscience, Wuhan, China) according to the manufacturer’s protocol respectively.

Techniques: Migration, Control, Standard Deviation, Concentration Assay, Expressing

Journal:

Article Title: A mutant, noninhibitory plasminogen activator inhibitor type 1 decreases matrix accumulation in experimental glomerulonephritis

doi: 10.1172/JCI200318038

Figure Lengend Snippet: Immunofluorescent staining score for ECM proteins at d6. Glomerular staining for FN-EDA+ (a), laminin (b), type I collagen (c), and type III collagen (d) were lower in the PAI-1R–treated, nephritic group. *P < 0.001 compared with normal control. #P < 0.01 compared with disease control.

Article Snippet: Monoclonal mouse anti-cellular fibronectin extra domain positive (FN-EDA+) (Harlan Sera-Lab Ltd., Loughborough, United Kingdom), rabbit anti–mouse laminin (ICN Immunobiologicals, Aurora, Ohio, USA), goat anti–human type I collagen, and goat anti–human type III collagen (Southern Biotechnology Associates, Birmingham, Alabama, USA) were used as the primary Ab’s for detection of matrix components in glomeruli at d6.

Techniques: Staining