Journal: PLoS ONE

Article Title: TACC3 Is Essential for EGF-Mediated EMT in Cervical Cancer

doi: 10.1371/journal.pone.0070353

Figure Lengend Snippet: ( A ) The expression of TACC3 in Ect1/E6E7 (HPV-immortalized ectocervical epithelial), CaSki (HPV-16), C33A (HPV-negative), SiHa (HPV-16) and HeLa (HPV-18) cell lines was determined by western blot analysis. The expression levels were compared to three normal cervix tissues. β-actin was used as a loading control. The intensity of bands was quantified using imageJ software and normalized to β-actin. Data shown are means ± SD of at least three independent experiments. ( B ) Representative immunohistochemical staining on cervical cancer tissue microarray. Quantitative analysis of cervical cancer tissue microarrays showed that the expression of TACC3 is higher in cervical cancer than in normal cervix, but its expression does not correlate with tumor stage ( C ) or grade ( D ). Data shown are means ± SD of at least three independent experiments. *, p <0.05; **, p <0.001.

Article Snippet: Human normal cervix tissue lysates were purchased from Imgenex (San Diego, CA).

Techniques: Expressing, Western Blot, Control, Software, Immunohistochemical staining, Staining, Microarray

Journal: PLoS ONE

Article Title: TACC3 Is Essential for EGF-Mediated EMT in Cervical Cancer

doi: 10.1371/journal.pone.0070353

Figure Lengend Snippet: ( A ) Representative immunohistochemical staining of TACC3 and Snail on cervical cancer tissue microarray. Quantitative analysis of cervical cancer tissue microarrays showed that ( B ) the expression of Snail is higher in cervical cancer than in normal cervix. Data shown are means ± SD of at least three independent experiments. *, p <0.001 ( C ) Snail expression correlates with TACC3 expression (r = 0.80383, p<0.0001 ).

Article Snippet: Human normal cervix tissue lysates were purchased from Imgenex (San Diego, CA).

Techniques: Immunohistochemical staining, Staining, Microarray, Expressing

Journal: Scientific Reports

Article Title: On the mechanism of miR-29b enhancement of etoposide toxicity in vitro

doi: 10.1038/s41598-024-70856-y

Figure Lengend Snippet: Effect of siRNA against Mcl-1 on etoposide cytotoxicity in different cell lines. (Panel a ): The cells were transfected (6 h) with siRNA against Mcl-1 or negative control. Cells were then treated with 60 μM etoposide 24 h post transfection and incubation continued until experiment was ended. The proliferation was calculated from doubling time value as explained in Materials and methods section. Each bar represents mean ± s.d. of at least three independent experiments (N HeLa = 4, N Hep G2 = 3, N Caco-2 = 3), in each experiment triplicate measurements were performed. **p < 0.01 versus corresponding negative control. (Panel b ): Evaluation of siRNA efficiency by western blot analysis in Hela cells. The cells were transfected (6 h) with anti-Mcl-1 siRNA or negative control. Shown are representative western blots of cells cultured for 24 h after transfection. (Panel c ): Evaluation of siRNA efficiency by western blot analysis in Hep G2 cells. Treatment was the same as for panel b. Panel d: Evaluation of siRNA efficiency by western blot analysis in Caco-2 cells. Treatment was the same as for (panel b ).

Article Snippet: HeLa cells were obtained from The European Collection of Authenticated Cell Cultures (ECACC, No. 93021013 Human Negroid cervix epitheloid carcinoma cell line) via Sigma-Aldrich as provider.

Techniques: Transfection, Negative Control, Incubation, Western Blot, Cell Culture

Journal: Scientific Reports

Article Title: Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors

doi: 10.1038/s41598-018-36527-5

Figure Lengend Snippet: Histological analysis of nsPEF treated tumors. 30–50 mm 3 B16F10 tumors were treated with 750, 200-ns pulses (25 kV/cm, 2 Hz) or left untreated (sham control). Panel A shows H&E pictures for one sham and one nsPEF-treated tumor collected at 4 h post treatment. In ( B ), both anti-cleaved caspase 3 (green) and -Ki-67 (red) immunofluorescence were performed to assess apoptosis and cell proliferation, respectively. Panel B shows representative images from three sham (top) and three nsPEF (bottom) -treated tumors. Panel C shows a positive control for the anti-cleaved Caspase 3 staining, namely HeLa cells treated with 1 μm staurosporin for 5 h. Scale bar: 1000 μm or 100 μm (inset) ( A ); 100 μm ( B , C ).

Article Snippet: Mouse melanoma B16F10 (ATCC, Manassas, Virginia) and human cervix carcinoma HeLa cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Corning®, Corning, NY) while the human lymphoma U-937 cell line was cultured in RPMI 1640 (Corning®).

Techniques: Immunofluorescence, Positive Control, Staining