human cd5l Search Results


human cd5l protein  (Sino Biological)
94
Sino Biological human cd5l protein
Human Cd5l Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd5l biotinylated antibody  (Bio-Techne corporation)
90
Bio-Techne corporation human cd5l biotinylated antibody
Human Cd5l Biotinylated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd5l biotinylated antibody - by Bioz Stars, 2026-07
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recombinant cd5l protein  (R&D Systems)
94
R&D Systems recombinant cd5l protein
Recombinant Cd5l Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/pmc02679343-39-6-14?v=R%26D+Systems
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recombinant cd5l protein - by Bioz Stars, 2026-07
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human rcd5l  (R&D Systems)
93
R&D Systems human rcd5l
Fig. 4. Recombinant CD5L <t>(rCD5L)</t> suppresses TGFβ-mediated fibrogenic responses in human lung fibroblast cells. Protein levels of COL1A1, FN1, and α-SMA in IMR-90 cells pre-treated with vehicle or rCD5L (0.4 μg/mL) for 3 h, followed by treatment with vehicle or TGFβ (2 ng/mL) for a further 24 h. Representative blots and quantification relative to α-tubulin are shown. Unpaired two-sample Student’s t-test (2-tailed) was used to analyze the difference between rCD5L-treated and vehicle-treated cells, either without or with TGFβ treatment.
Human Rcd5l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/pm40544764-40-0-3?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
human rcd5l - by Bioz Stars, 2026-07
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anti human aim polyclonal antibody  (R&D Systems)
94
R&D Systems anti human aim polyclonal antibody
Fig. 4. Recombinant CD5L <t>(rCD5L)</t> suppresses TGFβ-mediated fibrogenic responses in human lung fibroblast cells. Protein levels of COL1A1, FN1, and α-SMA in IMR-90 cells pre-treated with vehicle or rCD5L (0.4 μg/mL) for 3 h, followed by treatment with vehicle or TGFβ (2 ng/mL) for a further 24 h. Representative blots and quantification relative to α-tubulin are shown. Unpaired two-sample Student’s t-test (2-tailed) was used to analyze the difference between rCD5L-treated and vehicle-treated cells, either without or with TGFβ treatment.
Anti Human Aim Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/ppr0674778-54-10-15?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
anti human aim polyclonal antibody - by Bioz Stars, 2026-07
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aim cd5l nm 005894 human myc ddk tagged orf  (OriGene)
91
OriGene aim cd5l nm 005894 human myc ddk tagged orf
AIM and PSA expression levels are inversely correlated in patients with prostate cancer. Box and whisker plots of the differential expression of ( A ) <t>AIM/CD5L</t> and ( B ) PSA/KLK3 transcript expression in the prostate gland or prostate adenocarcinoma from the GSE6956 and GSE68907 cohort, respectively. ( C ) Comparative heatmap of the expression of KLK3 and CD5L in TCGA PRAD normal and tumor samples. ( D ) Box and whisker plots comparing the mRNA expression levels of AIM/CD5L and PSA/KLK3 in the TCGA PRAD cohort. ( E ) Representative IHC images showing the differential expression of AIM and AIM in normal prostate, low-grade, medium grade, and high-grade PCa samples from the TMU-SHH cohort. ( F ) Line and dots plot of the correlation between AIM and PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( left ). Line and dot plot showing the effect of CRISPR-mediated aim gene knockout, on PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( right ). TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; IHC, immunohistochemistry; TPM, transcripts per million.
Aim Cd5l Nm 005894 Human Myc Ddk Tagged Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/pmc08469063-45-8-16?v=OriGene
Average 91 stars, based on 1 article reviews
aim cd5l nm 005894 human myc ddk tagged orf - by Bioz Stars, 2026-07
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human gene knockout kit  (OriGene)
91
OriGene human gene knockout kit
AIM and PSA expression levels are inversely correlated in patients with prostate cancer. Box and whisker plots of the differential expression of ( A ) <t>AIM/CD5L</t> and ( B ) PSA/KLK3 transcript expression in the prostate gland or prostate adenocarcinoma from the GSE6956 and GSE68907 cohort, respectively. ( C ) Comparative heatmap of the expression of KLK3 and CD5L in TCGA PRAD normal and tumor samples. ( D ) Box and whisker plots comparing the mRNA expression levels of AIM/CD5L and PSA/KLK3 in the TCGA PRAD cohort. ( E ) Representative IHC images showing the differential expression of AIM and AIM in normal prostate, low-grade, medium grade, and high-grade PCa samples from the TMU-SHH cohort. ( F ) Line and dots plot of the correlation between AIM and PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( left ). Line and dot plot showing the effect of CRISPR-mediated aim gene knockout, on PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( right ). TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; IHC, immunohistochemistry; TPM, transcripts per million.
Human Gene Knockout Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/pm37100807-375-12-22?v=OriGene
Average 91 stars, based on 1 article reviews
human gene knockout kit - by Bioz Stars, 2026-07
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human cd5l  (Bio-Rad)
92
Bio-Rad human cd5l
Figure 4 Relative <t>CD5L</t> abundance in serum of patients with acute hypo- or
Human Cd5l, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/pm32183611-94-10-13?v=Bio-Rad
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human cd5l - by Bioz Stars, 2026-07
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differentiation 5 antigen like cd5l kits  (Cusabio)
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Cusabio differentiation 5 antigen like cd5l kits
The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
Differentiation 5 Antigen Like Cd5l Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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differentiation 5 antigen like cd5l kits - by Bioz Stars, 2026-07
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cdna of human cd5l  (GenScript corporation)
90
GenScript corporation cdna of human cd5l
The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
Cdna Of Human Cd5l, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd5l/us11370844-329-10-19?v=GenScript+corporation
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pci plasmid encoding cd5 leader (cd5l)–human immunoglobulin (hig) fusion protein  (Promega)
90
Promega pci plasmid encoding cd5 leader (cd5l)–human immunoglobulin (hig) fusion protein
The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
Pci Plasmid Encoding Cd5 Leader (Cd5l)–Human Immunoglobulin (Hig) Fusion Protein, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pci plasmid encoding cd5 leader (cd5l)–human immunoglobulin (hig) fusion protein - by Bioz Stars, 2026-07
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human cd5l / api6 protein  (Sino Biological)
94
Sino Biological human cd5l / api6 protein
The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups
Human Cd5l / Api6 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4. Recombinant CD5L (rCD5L) suppresses TGFβ-mediated fibrogenic responses in human lung fibroblast cells. Protein levels of COL1A1, FN1, and α-SMA in IMR-90 cells pre-treated with vehicle or rCD5L (0.4 μg/mL) for 3 h, followed by treatment with vehicle or TGFβ (2 ng/mL) for a further 24 h. Representative blots and quantification relative to α-tubulin are shown. Unpaired two-sample Student’s t-test (2-tailed) was used to analyze the difference between rCD5L-treated and vehicle-treated cells, either without or with TGFβ treatment.

Journal: Biochemical and biophysical research communications

Article Title: CD5L is a target of transcription factor Nrf2.

doi: 10.1016/j.bbrc.2025.152225

Figure Lengend Snippet: Fig. 4. Recombinant CD5L (rCD5L) suppresses TGFβ-mediated fibrogenic responses in human lung fibroblast cells. Protein levels of COL1A1, FN1, and α-SMA in IMR-90 cells pre-treated with vehicle or rCD5L (0.4 μg/mL) for 3 h, followed by treatment with vehicle or TGFβ (2 ng/mL) for a further 24 h. Representative blots and quantification relative to α-tubulin are shown. Unpaired two-sample Student’s t-test (2-tailed) was used to analyze the difference between rCD5L-treated and vehicle-treated cells, either without or with TGFβ treatment.

Article Snippet: Human rCD5L (2797-CL, R&D Systems) was reconstituted in sterile PBS.

Techniques: Recombinant

AIM and PSA expression levels are inversely correlated in patients with prostate cancer. Box and whisker plots of the differential expression of ( A ) AIM/CD5L and ( B ) PSA/KLK3 transcript expression in the prostate gland or prostate adenocarcinoma from the GSE6956 and GSE68907 cohort, respectively. ( C ) Comparative heatmap of the expression of KLK3 and CD5L in TCGA PRAD normal and tumor samples. ( D ) Box and whisker plots comparing the mRNA expression levels of AIM/CD5L and PSA/KLK3 in the TCGA PRAD cohort. ( E ) Representative IHC images showing the differential expression of AIM and AIM in normal prostate, low-grade, medium grade, and high-grade PCa samples from the TMU-SHH cohort. ( F ) Line and dots plot of the correlation between AIM and PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( left ). Line and dot plot showing the effect of CRISPR-mediated aim gene knockout, on PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( right ). TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; IHC, immunohistochemistry; TPM, transcripts per million.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: AIM and PSA expression levels are inversely correlated in patients with prostate cancer. Box and whisker plots of the differential expression of ( A ) AIM/CD5L and ( B ) PSA/KLK3 transcript expression in the prostate gland or prostate adenocarcinoma from the GSE6956 and GSE68907 cohort, respectively. ( C ) Comparative heatmap of the expression of KLK3 and CD5L in TCGA PRAD normal and tumor samples. ( D ) Box and whisker plots comparing the mRNA expression levels of AIM/CD5L and PSA/KLK3 in the TCGA PRAD cohort. ( E ) Representative IHC images showing the differential expression of AIM and AIM in normal prostate, low-grade, medium grade, and high-grade PCa samples from the TMU-SHH cohort. ( F ) Line and dots plot of the correlation between AIM and PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( left ). Line and dot plot showing the effect of CRISPR-mediated aim gene knockout, on PSA transcript expression in prostate cancer cell lines from the DepMap Public 21Q2 dataset ( right ). TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; IHC, immunohistochemistry; TPM, transcripts per million.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Whisker Assay, Quantitative Proteomics, CRISPR, Gene Knockout, Immunohistochemistry

Aberrantly expressed PSA and significantly suppressed AIM expression characterize advanced stage prostate cancer, regardless of mutation status. Box and whisker and dot plots of the expression profiles of ( A ) PSA/KLK3 and ( B ) AIM/CD5L in normal tissue, early stage, or advanced stage disease across 32 cancer types from the ICR-curated dataset. Graphical representation of cases affected by ( C ) psa/klk3 and ( D ) aim/cd5l mutation ( upper ) or CNV ( lower ) in ICR-curated pan-cancer TCGA cohort data. ICR, Institute of Cancer Research; CNV, copy number variation; TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; red box, PCa/PRAD cohort.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: Aberrantly expressed PSA and significantly suppressed AIM expression characterize advanced stage prostate cancer, regardless of mutation status. Box and whisker and dot plots of the expression profiles of ( A ) PSA/KLK3 and ( B ) AIM/CD5L in normal tissue, early stage, or advanced stage disease across 32 cancer types from the ICR-curated dataset. Graphical representation of cases affected by ( C ) psa/klk3 and ( D ) aim/cd5l mutation ( upper ) or CNV ( lower ) in ICR-curated pan-cancer TCGA cohort data. ICR, Institute of Cancer Research; CNV, copy number variation; TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma; red box, PCa/PRAD cohort.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Mutagenesis, Whisker Assay

The differential expression of PSA and AIM is associated with disease recurrence in patients with prostate cancer but is equivocal for overall survival. Kaplan–Meier curves showing the effect of high or low ( A ) PSA and ( B ) AIM expression on BCR-free survival in the TCGA PRAD cohort. Kaplan–Meier curves showing the effect of high or low ( C ) PSA and ( D ) AIM expression on overall survival in the TCGA PRAD cohort. BCR risk group-stratified heatmap ( left ) and box-and-whisker plots ( right ) of ( E ) PSA/KLK3 or ( F ) AIM/CD5L expression levels in the GSE40272 cohort. ( G ) XY plot showing the differential expression of AIM and PSA in the GSE within the Broad_2020_09_c7_immunologic gene set. BCR, biochemical recurrence; TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: The differential expression of PSA and AIM is associated with disease recurrence in patients with prostate cancer but is equivocal for overall survival. Kaplan–Meier curves showing the effect of high or low ( A ) PSA and ( B ) AIM expression on BCR-free survival in the TCGA PRAD cohort. Kaplan–Meier curves showing the effect of high or low ( C ) PSA and ( D ) AIM expression on overall survival in the TCGA PRAD cohort. BCR risk group-stratified heatmap ( left ) and box-and-whisker plots ( right ) of ( E ) PSA/KLK3 or ( F ) AIM/CD5L expression levels in the GSE40272 cohort. ( G ) XY plot showing the differential expression of AIM and PSA in the GSE within the Broad_2020_09_c7_immunologic gene set. BCR, biochemical recurrence; TCGA, the cancer genome atlas; PRAD, prostate adenocarcinoma.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Quantitative Proteomics, Expressing, Whisker Assay

PSA-associated suppression of AIM is implicated in the enhanced metastability of prostate cancer and a high AIM/PSA ratio is associated with strong castration-induced regression. Histograms showing the differential expression of ( A ) cd5l/aim and ( B ) klk3/psa in poorly metastatic or highly metastatic sample from the GDS2865 dataset. ( C ) Histogram comparing the cd5l and klk3 expression values in the poorly or highly metastatic samples from the GDS2865 dataset. Histograms showing the differential expression of ( D ) cd5l/aim and ( E ) klk3/psa in androgen-dependent growth, castration-induced regression nadir, or castration-resistant regrowth samples from the GDS4107 dataset. ( F ) Histograms of the cd5l/klk3 ratio in androgen-dependent growth, castration-induced regression nadir, or castration-resistant regrowth samples from the GDS4107 dataset. ( G ) Sample percentage bin plot of cd5l and klk3 expression in the GSE46691 cohort stratified by propensity for metastasis. ( H ) Box and whisker plots of the differential expression levels of CD5L ( upper left ) and KLK3 ( upper right ) in patients with or without AR-amplification in the MET500 PRAD cohort. Box and whisker plots of the differential expression levels of CD5L ( lower left ) and KLK3 ( lower right ) in patients with or without ERG fusion in the MET500 PRAD cohort. ( I ) Representative photomicrographs ( upper ) and quantitative chart ( lower ) showing the effect of CD5L_OE on invasion of PC3 or DU145 cells. ( J ) Representative photomicrographs ( upper ) and histograms ( lower ) showing the effect of CD5L_OE on migration of PC3 cells at indicated time-points. CD5L_OE, overexpression of cd5l ; AR, androgen receptor; F.C., fold change; ** p < 0.01; *** p < 0.001.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: PSA-associated suppression of AIM is implicated in the enhanced metastability of prostate cancer and a high AIM/PSA ratio is associated with strong castration-induced regression. Histograms showing the differential expression of ( A ) cd5l/aim and ( B ) klk3/psa in poorly metastatic or highly metastatic sample from the GDS2865 dataset. ( C ) Histogram comparing the cd5l and klk3 expression values in the poorly or highly metastatic samples from the GDS2865 dataset. Histograms showing the differential expression of ( D ) cd5l/aim and ( E ) klk3/psa in androgen-dependent growth, castration-induced regression nadir, or castration-resistant regrowth samples from the GDS4107 dataset. ( F ) Histograms of the cd5l/klk3 ratio in androgen-dependent growth, castration-induced regression nadir, or castration-resistant regrowth samples from the GDS4107 dataset. ( G ) Sample percentage bin plot of cd5l and klk3 expression in the GSE46691 cohort stratified by propensity for metastasis. ( H ) Box and whisker plots of the differential expression levels of CD5L ( upper left ) and KLK3 ( upper right ) in patients with or without AR-amplification in the MET500 PRAD cohort. Box and whisker plots of the differential expression levels of CD5L ( lower left ) and KLK3 ( lower right ) in patients with or without ERG fusion in the MET500 PRAD cohort. ( I ) Representative photomicrographs ( upper ) and quantitative chart ( lower ) showing the effect of CD5L_OE on invasion of PC3 or DU145 cells. ( J ) Representative photomicrographs ( upper ) and histograms ( lower ) showing the effect of CD5L_OE on migration of PC3 cells at indicated time-points. CD5L_OE, overexpression of cd5l ; AR, androgen receptor; F.C., fold change; ** p < 0.01; *** p < 0.001.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Quantitative Proteomics, Expressing, Whisker Assay, Amplification, Migration, Over Expression

The functional association between PSA and AIM modulates metastasis and mirrors the immunogenicity in patients with prostate cancer. Box and whisker plots showing the differential expression of ( A ) cd5l/aim and ( B ) psa/klk3 genes normal, primary tumor, or metastatic samples from the TNMplot gene chip data. ( C ) Expression heatmap showing the correlation between CD5L , KLK3 , with biomarkers of cancer stemness, epithelial-to-mesenchymal transition, metastasis, or T-cell activation in normal, localized PCa, and bone metastatic CRPC in the GSE32269. ( D ) Graphical representation of the differential expression of CD5L , KLK3 , biomarkers of M1 phenotype, M2 phenotype, and T-cell activation in non-tumor or tumor samples from the TCGA PRAD cohort.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: The functional association between PSA and AIM modulates metastasis and mirrors the immunogenicity in patients with prostate cancer. Box and whisker plots showing the differential expression of ( A ) cd5l/aim and ( B ) psa/klk3 genes normal, primary tumor, or metastatic samples from the TNMplot gene chip data. ( C ) Expression heatmap showing the correlation between CD5L , KLK3 , with biomarkers of cancer stemness, epithelial-to-mesenchymal transition, metastasis, or T-cell activation in normal, localized PCa, and bone metastatic CRPC in the GSE32269. ( D ) Graphical representation of the differential expression of CD5L , KLK3 , biomarkers of M1 phenotype, M2 phenotype, and T-cell activation in non-tumor or tumor samples from the TCGA PRAD cohort.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Functional Assay, Immunopeptidomics, Whisker Assay, Quantitative Proteomics, Expressing, Activation Assay

Heatmap showing the correlation between CD5L , KLK3 , with ( A ) biomarkers of positive regulation of T-cell responses, pro-inflammatory markers, Treg markers, ( B ) T-cell activation markers, and ( C ) macrophage markers, in normal, localized PCa, and bone metastatic CRPC in the GSE32269. Columns with similar annotations are collapsed by taking mean inside each group. Rows are centered; unit variance scaling is applied to rows. Both rows and columns are clustered using correlation distance and average linkage. Varied rows, three columns. ( D ) Graphical visualization of the five clusters formed by cd5l and klk3 gene-enrichment-based molecular or biological process functional annotation clustering using a medium classification stringency. ( E ) Gene ontology visualization of reported gene-term association. Treg, regulatory T cell; CRPC, castration-resistant prostate cancer.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: Heatmap showing the correlation between CD5L , KLK3 , with ( A ) biomarkers of positive regulation of T-cell responses, pro-inflammatory markers, Treg markers, ( B ) T-cell activation markers, and ( C ) macrophage markers, in normal, localized PCa, and bone metastatic CRPC in the GSE32269. Columns with similar annotations are collapsed by taking mean inside each group. Rows are centered; unit variance scaling is applied to rows. Both rows and columns are clustered using correlation distance and average linkage. Varied rows, three columns. ( D ) Graphical visualization of the five clusters formed by cd5l and klk3 gene-enrichment-based molecular or biological process functional annotation clustering using a medium classification stringency. ( E ) Gene ontology visualization of reported gene-term association. Treg, regulatory T cell; CRPC, castration-resistant prostate cancer.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Activation Assay, Functional Assay

AIM binds directly to PSA, and the ectopic (re)expression of AIM enhances the anticancer therapeutic effect of Pembrolizumab against PCa cells. 2D visualization of the auto-generated ( A ) CD5L-KLK3 interactome showing complicity in the immune landscape, macrophages, EMT and CSC regulation, ( B ) CD5L interactome, or KLK3 interactome. ( C ) Three-dimensional visualization of the direct complex formation by AIM/CD5L (surface model) and PSA/KLK3 (ribbon model). ( D ) Line graph showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on PC3 cell proliferation. ( E ) Representative photomicrographs ( upper ) and histograms ( lower ) showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on PC3 cell invasion. ( F ) Representative photomicrographs ( upper ) and quantitative chart ( lower ) showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on the tumorsphere formation capability of PC3 cells. ( G ) Representative IHC photomicrographs ( upper ) and histograms ( lower ) of the immunoreactivity of AIM, PSA, and PDL1 in pembro_responders or pembro_non-responders from the TMU-SHH cohort. ( H ) Histograms showing the differential CD8 + T-cell count/field in pembro_responders or pembro_non-responders from the TMU-SHH cohort. WT, wild type; OE, overexpression; * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Biomedicines

Article Title: Concomitant High Apoptosis Inhibitor of Macrophage (AIM) and Low Prostate-Specific Antigen (PSA) Indicates Activated T Cell-Mediated Anticancer Immunity, Enhance Sensitivity to Pembrolizumab, and Elicit Good Prognosis in Prostate Cancer

doi: 10.3390/biomedicines9091225

Figure Lengend Snippet: AIM binds directly to PSA, and the ectopic (re)expression of AIM enhances the anticancer therapeutic effect of Pembrolizumab against PCa cells. 2D visualization of the auto-generated ( A ) CD5L-KLK3 interactome showing complicity in the immune landscape, macrophages, EMT and CSC regulation, ( B ) CD5L interactome, or KLK3 interactome. ( C ) Three-dimensional visualization of the direct complex formation by AIM/CD5L (surface model) and PSA/KLK3 (ribbon model). ( D ) Line graph showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on PC3 cell proliferation. ( E ) Representative photomicrographs ( upper ) and histograms ( lower ) showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on PC3 cell invasion. ( F ) Representative photomicrographs ( upper ) and quantitative chart ( lower ) showing the effect of CD5L_OE with or without 5 μM Pembrolizumab on the tumorsphere formation capability of PC3 cells. ( G ) Representative IHC photomicrographs ( upper ) and histograms ( lower ) of the immunoreactivity of AIM, PSA, and PDL1 in pembro_responders or pembro_non-responders from the TMU-SHH cohort. ( H ) Histograms showing the differential CD8 + T-cell count/field in pembro_responders or pembro_non-responders from the TMU-SHH cohort. WT, wild type; OE, overexpression; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Ectopic expression of AIM/CD5L was achieved using the AIM (CD5L) (NM_005894) Human (Myc-DDK)-Tagged ORF Clone (#RC206528L2, OriGene Technologies, Inc., Rockville, MD, USA) in pCMV6-Entry vector transfected into PC-3 cells using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Generated, Cell Counting, Over Expression

Figure 4 Relative CD5L abundance in serum of patients with acute hypo- or

Journal: Thyroid : official journal of the American Thyroid Association

Article Title: CD5L Constitutes a Novel Biomarker for Integrated Hepatic Thyroid Hormone Action.

doi: 10.1089/thy.2019.0635

Figure Lengend Snippet: Figure 4 Relative CD5L abundance in serum of patients with acute hypo- or

Article Snippet: CD5L was detected by rabbit antibodies against the N-terminus of human CD5L (AHP1169, Bio-Rad Laboratories GmbH, Feldkirchen, Germany) in combination with goat anti-rabbit-immunoglobulin-HRP antibody (P0448, Agilent Dako, Santa Clara, United States).

Techniques:

The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Clinical Proteomics

Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Biomarker Discovery, Comparison