Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: a Experimental design. Blood samples were collected at 7:00 am ± 15 min ( n = 10) or 11:30 am ± 15 min ( n = 10) from recipients with malignant hematological diseases in complete remission prior to cord blood infusion. b , c Serum concentrations of cytokines before cord blood infusion. Normalized z score ( b ) and absolute concentrations ( c ) of cytokines between 7:00 am and 11:30 am. The P values are two-sided and reported as exact values. d Absolute concentrations of soluble CD26/DPPIV in serum post-MAC. Blood samples were collected from recipients at 7:00 am ± 15 min ( n = 19), 11:30 am ± 15 min ( n = 19), or 4:00 pm ± 15 min ( n = 29). e – i Associations between serum soluble CD26/DPPIV (sCD26/DPPIV) levels and inflammatory cytokines before cord blood infusion on day 0. Pearson’s correlation coefficient ( r ) was calculated for the relationships between serum DPPIV levels and IL-1Ra ( e ) ( r = 0.476, P = 0.034), IL-1β ( f ) ( r = 0.450, P = 0.046), LIF ( g ) ( r = 0.517, P = 0.020), IL-18 ( h ) ( r = −0.811, P < 0.001) and IL-1α ( i ) ( r = 0.006, P = 0.741) concentrations. The P values are two-sided and reported as exact values.The data are presented as the means ± SEMs and were analyzed by unpaired t test followed by Bonferroni-Dunn correction ( c ), one-way ANOVA with Bonferroni multiple comparisons ( d ) and Pearson’s correlation ( e – i ). Source data are provided as a Source Data file.

Article Snippet: In the experimental groups, recombinant human CD26/DPPIV protein (1000 ng/mL, 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (DPPIV inhibitor, 200 μg/mL, S4002, Selleck) was added to the respective groups.

Techniques:

Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: a , b Representative immunofluorescence images ( a ) and quantification ( b ) of CD26/DPPIV levels in intestinal epithelial cells from a healthy population at different times ( n = 3 patients per group) ( P < 0.001). Intestinal samples were collected at 10 am (CT10), 2 pm (CT14), and 6 pm (CT18). Scale bars, 50 μm. c Bmal1 and DPP4 expression in primary mouse epidermal cells postsynchronization in vitro ( n = 3 biological replicates, each from an independent primary culture prepared and synchronized on separate days). The Gapdh gene was used as an internal control. The relative expression levels were computed via the 2 −ΔΔCT method. CT6 is double plotted. d The concentration of CD26/DPPIV in the supernatant of primary mouse epidermal cells postsynchronization in vitro ( n = 3 biological replicates, each from an independent primary culture prepared and synchronized on separate days) ( P < 0.001). CT6 is double plotted. e Diurnal mRNA expression of DPP4 in mouse epidermal cells. Bmal1 ΔEC , epithelium cell-specific Bmal1 knockout mice. Statistics were obtained from a publicly available dataset ( GSE190035 ), with values representing the log2-transformed mean RPKM from 4 biological replicates per time point. ZT0 is double plotted.The data are presented as the means ± SEMs and were analyzed by one-way ANOVA with Bonferroni multiple comparisons ( b , d ) and JTK_Cycle ( c , e ). Source data are provided as a Source Data file.

Article Snippet: In the experimental groups, recombinant human CD26/DPPIV protein (1000 ng/mL, 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (DPPIV inhibitor, 200 μg/mL, S4002, Selleck) was added to the respective groups.

Techniques: Immunofluorescence, Expressing, In Vitro, Control, Concentration Assay, Knock-Out, Transformation Assay

Journal: Nature Communications

Article Title: Circadian fluctuation of soluble CD26 dictates the impact of the timing of cord blood transplantation on acute graft-versus-host disease

doi: 10.1038/s41467-026-68958-4

Figure Lengend Snippet: PBMCs from healthy donors were cultured with PHA-L (control group), PHA-L plus CD26/DPPIV (sCD26 group), or PHA-L plus Sitagliptin (DPPIV inhibitor, DPPIVi group) for 72 h. a Experimental design. PBMCs from healthy donors were cultured with PHA-L (control group), PHA-L plus CD26/DPPIV (sCD26 group), or PHA-L plus Sitagliptin (DPPIV inhibitor, DPPIVi group) for 72 h. b , c Representative plots (left) and quantified percentages (right) of CD86 + ( b ) and CD80 + ( c ) CD14 + cells after 72 h of PBMCs coculture in different groups (pooled data from 8 healthy donors across four independent experiments). d – i Representative plots (left) and quantified percentages (right) of Ki-67 + , IFNγ + , and CD38 + cells among CD4 + T cells ( d , f , h ) and CD8 + T cells ( e , g , i ) after 72 h of PBMCs coculture in different groups (pooled data from 6 healthy donors across three independent experiments).The data are presented as the means ± SEMs and were analyzed by one-way ANOVA with the Bonferroni correction for multiple comparisons ( b – i ). All P values are two-sided and reported as exact values unless <0.001. Source data are provided as a Source Data file.

Article Snippet: In the experimental groups, recombinant human CD26/DPPIV protein (1000 ng/mL, 11244-SE, R&D Systems) or Sitagliptin phosphate monohydrate (DPPIV inhibitor, 200 μg/mL, S4002, Selleck) was added to the respective groups.

Techniques: Cell Culture, Control

Journal: Oncotarget

Article Title: Synergistic cytotoxic activity of cannabinoids from cannabis sativa against cutaneous T-cell lymphoma (CTCL) in-vitro and ex-vivo

doi: 10.18632/oncotarget.27528

Figure Lengend Snippet: PBL were isolated from blood samples of Sézary patients ( n = 6), and were treated with S4 (5 μg/mL), S5 (6 μg/mL), S4 (5 μg/mL) +S5 (6 μg/mL) and control (methanol; Vehicle treatment) for 48 h. Cells were harvested and analyzed by FACS following CD4-APC, CD26-alexa 405, Annexin V-FITC and PI staining. Apoptotic-induced cells (Annexin positive cells) were determined in the CD4 + CD26 - cell population and in non-CD4 + CD26 - cells of treated cells minus control. ( A ) The percent of apoptotic-induced CD4 + CD26 - cells is presented for single treatment compared to combined treatment; ( n = 4); *** denote significant difference between means (one way ANOVA; p < 0.001). ( B ) The percent of apoptotic-induced cells following the combined treatment was compared between CD4 + CD26 - cells and non-CD4 + CD26 - cells of SPBL; ( n = 6); ** denote significant difference between means (paired student T test; 0.001 < P < 0.05).

Article Snippet: Cells were suspended in 100 μL binding buffer with 1 μL Annexin V-FITC (4830-01-1, eBioscience) + 2 μL CD26 Alexa Fluor 405-conjugated antibody (FAB1180V-100UG, R&D SYSTEMS) + 10 μL CD4-APC-conjugated antibody (FAB3791A-100, R&D SYSTEMS) and incubated for 15 min at room temperature.

Techniques: Isolation, Control, Staining

Journal: Journal of Virology

Article Title: Differential Expression of the Middle East Respiratory Syndrome Coronavirus Receptor in the Upper Respiratory Tracts of Humans and Dromedary Camels

doi: 10.1128/jvi.02994-15

Figure Lengend Snippet: Figure 1. DPP4 expression in the upper respiratory tract of camels and humans. DPP4 293

Article Snippet: DPP4 was detected using 5 μg/ml polyclonal goat IgG anti-human DPP4 antibody 82 (R&D systems, Abingdon, UK), while negative controls were stained using normal goat serum 83 (MP Biomedicals, Santa Ana, CA, USA) in equal concentration.

Techniques: Expressing

Journal: Cells

Article Title: Avian Reovirus P17 Suppresses Angiogenesis by Promoting DPP4 Secretion

doi: 10.3390/cells10020259

Figure Lengend Snippet: ARV p17-mediated release of Dipeptidyl Peptidase 4 (DPP4) expression. ( A ) Wound healing assay performed after HUVECs’ overnight stimulation with conditioned medium from Mock- or ARV p17-nucleofected HUVEC cells. Confluent cell monolayers were scratched using a 200 μL pipette tip and cell migration was recorded by light microscopy 10 h after wound scratch (original magnification, 4×). The wound width was measured, and the relative wound area was calculated as the ratio of the remaining area at the 10 h time point to the 0 h starting point. Pictures are representative of one out of two independent experiments with similar results. Values are the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( B ) Tube formation assay performed with HUVECs co-cultivated for 48 h with Mock- or ARV p17-nucleofected HUVEC cells. The pictures were taken 6 h after cell seeding (original magnification, 4×). Closed rings were counted as a parameter for quantification of tube formation. Pictures are representative of one out of two independent experiments with similar results. Values are the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( C ) Angiogenesis array performed with the supernatants of Mock- or ARV p17-nucleofected cells recovered 24 h post-nucleofection. Relative pixel intensity was calculated using ImageJ software and expressed as the mean of duplicate dots. Values are representative of one experiment out of two with similar results. ( D ) Analysis of DPP4 gene expression performed using quantitative real-time PCR in Mock- and ARV p17-nucleofected cells. Analysis of real-time PCR data were performed with the 2 -DDCt method using relative quantitation study software. Quantification of DPP4 mRNA was normalized according to the internal β-actin control. Values represent the mean ± SD of one representative experiment out of two with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001. ( E ) soluble (s) DPP4 levels in supernatants of HUVECs stimulated with GST-ARV p17 for 48 h were quantified with human sDPP4 ELISA. Supernatant from GST stimulated cells was used as a negative control. Bar graph displays the concentration of DPP4 determined by absorbance quantification. Values are the mean ± SD of one representative experiment out of three independent experiments with similar results, performed in triplicates. Statistical analysis was performed by Student’s t test. *** p < 0.001.

Article Snippet: The release of DPP4 in the concentrated conditioned medium was evaluated using a human CD26 ELISA Kit (R&D systems) according to manufacturer’s instructions.

Techniques: Expressing, Wound Healing Assay, Transferring, Migration, Light Microscopy, Tube Formation Assay, Software, Gene Expression, Real-time Polymerase Chain Reaction, Quantitation Assay, Control, Enzyme-linked Immunosorbent Assay, Negative Control, Concentration Assay

Journal: BMC Endocrine Disorders

Article Title: Adiponectin, chemerin, cytokines, and dipeptidyl peptidase 4 are released from human adipose tissue in a depot-dependent manner: an in vitro system including human serum albumin

doi: 10.1186/1472-6823-14-7

Figure Lengend Snippet: Release of adiponectin, IL-6, chemerin, and DPP4 from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). Concentrations of adiponectin, IL-6, chemerin and DPP4 in media from paired biopsies of subcutaneous and visceral adipose tissue incubated in medium containing 1% HSA according to our final protocol were determined by ELISA. Adiponectin was more abundantly released from subcutaneous than visceral adipose tissue (p < 0.01, n = 7) (A) . IL-6 release from the depots was similar (p = 0.315, n = 11) (B) , while chemerin (C) and DPP4 (D) were more abundantly released from visceral adipose tissue (p < 0.05 and p < 0.001, respectively, n = 11).

Article Snippet: ELISA was used to measure adiponectin (Human adiponectin ELISA kit, Millipore, Billerica, MA), chemerin (Human chemerin ELISA kit, Millipore), omentin (Human omentin-1 ELISA, Millipore), IL-6 (Quantikine High Sensitivity Human IL-6 ELISA, R&D Systems, Minneapolis, MN), and DPP4 (Quantikine Human DPPIV/CD26 ELISA kit, R&D Systems).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay