Journal: International Journal of Molecular Sciences

Article Title: Cell State Transitions and Phenotypic Heterogeneity in Luminal Breast Cancer Implicating MicroRNAs as Potential Regulators

doi: 10.3390/ijms24043497

Figure Lengend Snippet: Expression profiling of stemness-associated markers in breast cancer. ( A ) Breast cancer cell lines were screened for the expression of the cell surface marker CD147/Basigin or EMMPRIN, CD151/tetraspanin, and drug transporter proteins CD243/ABCB1 and CD338/ABCG2. More than 5% of MCF7, BT474, and SKBR3 cells expressed drug transporter proteins (* comparison between unstained control and stained cells for each cell line, p ≤ 0.05; calculated by Student’s t -test). ( B ) Immunofluorescence staining for CD147 and CD151 (scale bar measures 75 µm). ( C ) Breast cancer cell lines MCF7, BT474, and SKBR3 showed positive expression for aldehyde dehydrogenase activity (ALDH), indicating the enrichment of cells with higher self-renewal and drug resistance potential.

Article Snippet: The panel of antibodies used for phenotypic characterization were CD147-FITC (Clone REA282), CD151-PE (Clone REA265), CD243/ABCB1-Viobright FITC (Clone REA495), and CD338/ABCG2-PerCP_Vio700 (Clone 5D3), purchased from Miltenyi Biotec, Gaithersburg, MD, USA).

Techniques: Expressing, Marker, Comparison, Control, Staining, Immunofluorescence, Activity Assay

Journal: Cell Reports Medicine

Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases

doi: 10.1016/j.xcrm.2024.101755

Figure Lengend Snippet:

Article Snippet: APC-conjugated anti-human TRA-1-85 (CD147) , Miltenyi Biotec , Cat#130-128-900; RRID:AB_2921968.

Techniques: Virus, Recombinant, Staining, Labeling, Electron Microscopy, Bradford Assay, RNA Sequencing, Gene Expression, Knock-Out, CRISPR, Luciferase, Software, Fluorescence

Journal: Oncotarget

Article Title: Tumour-associated circulating microparticles: A novel liquid biopsy tool for screening and therapy monitoring of colorectal carcinoma and other epithelial neoplasia

doi: 10.18632/oncotarget.9018

Figure Lengend Snippet: Measured EpCAM + taMP and EpCAM + CD147 + taM P values were set in dependence (Spearman algorithm) to associate patient tumour volumes of indicated cancer entities; ( A–B ): NSCLC; ( C–F ): CRC. Correlations were restricted to 100 cm 3 of tumour volume. (D/F) Detail analysis of indicated tumour ranges revealing the possible lower and upper detection limit. Shown are indicated median with 25 and 95 percentile including p -value as indicated; * = p < 0.05, ** = p < 0.005, *** = p < 0.0005, n.s. = not significant (one-way ANOVA test including multiple comparisons using Dunn's Multiple Comparison Test). Overall, an error level p < 0.05 was considered significant. Calculations were done with Prism 5 (GraphPad Software, Inc., USA).

Article Snippet: MPs sedimenting at 20,000 g were characterized by FACS using staining for AnnexinV, CD147, EpCAM (eBioscienceTM, San Diego, CA; BioLegend; Miltenyi Biotec, Bergisch Gladbach, Germany, respectively).

Techniques: Comparison, Software

Journal: Oncotarget

Article Title: Tumour-associated circulating microparticles: A novel liquid biopsy tool for screening and therapy monitoring of colorectal carcinoma and other epithelial neoplasia

doi: 10.18632/oncotarget.9018

Figure Lengend Snippet: ( A–B ) Measured EpCAM + taMP and EpCAM + CD147 + taMP values were set in dependence (Spearman algorithm) to associate patient UICC values. ( C–D ) Direct comparison of indicated taMPs population in metastatic CRC (mCRC) vs. non-metastatic CRC and healthy controls. (E/F) Direct comparison of measured CEA and CA 19–9 values in ng/mL in metastatic CRC (mCRC) vs. non-metastatic CRC. Shown are indicated median with 25 and 95 percentile including p -value as indicated; * = p < 0.05, ** = p < 0.005, *** = p < 0.0005, n.s. = not significant (one-way ANOVA test including multiple comparisons using Dunn's Multiple Comparison Test (C/D) or unpaired Mann-Whitney test (E/F)).

Article Snippet: MPs sedimenting at 20,000 g were characterized by FACS using staining for AnnexinV, CD147, EpCAM (eBioscienceTM, San Diego, CA; BioLegend; Miltenyi Biotec, Bergisch Gladbach, Germany, respectively).

Techniques: Comparison, MANN-WHITNEY

Journal: Oncotarget

Article Title: Tumour-associated circulating microparticles: A novel liquid biopsy tool for screening and therapy monitoring of colorectal carcinoma and other epithelial neoplasia

doi: 10.18632/oncotarget.9018

Figure Lengend Snippet: ( A ) EpCAM + taMPs levels 7 days post CRC tumour resection and at day 10 post-OP. ( B ) Paired display of accompanied post-OP and post-OP values of indicated taMP populations. ( C ) EpCAM + CD147 + taMPs levels 7 days post CRC tumour resection and at day 10 post-OP. ( D ) CD147 + EpCAM − taMPs levels 7 days post CRC tumour resection and at day 10 post-OP. Shown are indicated median with 25 and 95 percentile including p -value as indicated; * = p < 0.05, ** = p < 0.005, *** = p < 0.0005, n.s. = not significant (paired t -test with Wilcoxon matched pairs signed rank-test). Overall, an error level p < 0.05 was considered significant. ( E ) Sketch illustrating the heterogeneous tumour composition and tumour antigens used in the current study. Tumour cells shed multiple MP subpopulations carrying a distinguishing set of surface markers. These shedded taMPs can be detected in the sera of cancer patients.

Article Snippet: MPs sedimenting at 20,000 g were characterized by FACS using staining for AnnexinV, CD147, EpCAM (eBioscienceTM, San Diego, CA; BioLegend; Miltenyi Biotec, Bergisch Gladbach, Germany, respectively).

Techniques: