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Image Search Results
Journal: iScience
Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer
doi: 10.1016/j.isci.2022.103758
Figure Lengend Snippet: rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and Arg1 staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82),
Techniques: Immunofluorescence, Staining, Cell Culture, In Vitro
Journal: iScience
Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer
doi: 10.1016/j.isci.2022.103758
Figure Lengend Snippet: βig-h3 Ab depletion in vivo reprograms macrophage phenotype in the tumor microenvironment (A) Experimental setting. (B) Tumor weight of mice treated in A. (C-F) FACS analysis of the percentages of CD45 + (C), F4/80 + CD45 + (D), CD206 + F4/80 + (E), and Arg1 + F4/80 + (F) cells collected in A. ∗p<0.05, ∗∗p<0.01. Representative of two independent experiments with fivemice per group.
Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82),
Techniques: In Vivo
Journal: iScience
Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer
doi: 10.1016/j.isci.2022.103758
Figure Lengend Snippet:
Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82),
Techniques: Recombinant, Modification, Plasmid Preparation, Activation Assay, Concentration Assay, Software