human β-actin Search Results


g actin  (Cytoskeleton Inc)
95
Cytoskeleton Inc g actin
G Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti aacs in house  (R&D Systems)
96
R&D Systems anti aacs in house
Anti Aacs In House, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β actin  (Bio-Rad)
93
Bio-Rad β actin
Figure 1. Transcriptional reprogramming of neuronal metabolism during glucose deprivation. (A) Schematic for glucose deprivation of rat cortical neurons. A subset of ∼250 genes significantly upregulated during glucose deprivation (P value <0.01) were selected for pathway analysis using annotated gene sets from the Molecular Signatures Database (MSigDB). n = 3 cortical samples from one rat litter. (B) Enrichment of genes in MSigDB Reactome pathways with adjusted P value <0.05. (C) Enrichment of the target genes of the CREB transcription factor in glucose-deprived neurons (adjusted P value <0.05). The target gene Pgc1α (PPARGC1A) is highlighted in red. (D) Immunostaining of cortical neuronal cultures (treated as in A) with an anti-phospho-CREB antibody and Hoechst nuclear stain. Arrowheads denote pCREB-positive nuclei. (E) Fraction of nuclei with positive p-CREB staining in fields of view (FOV), determined as described in Materials and methods. % total ± SEM: +glucose (3 h), 14.87 ± 2.29, −glucose (3 h), 27.41 ± 3.3. n = 17–22 FOVs. (F) Relative mRNA expression of Pgc1α in neuronal cultures treated as in A, with or without the AMPK inhibitor, dorsomorphin. Values are normalized to <t>β-actin</t> mRNA and expressed relative to the +glucose condition. Average normalized mRNA level ± SEM: −glucose (1 h), 2.83 ± 0.42; −glucose (3 h), 3.67 ± 0.46; −glucose + dorsomorphin (3 h), 1.32 ± 0.22. n = 3–10 cortical samples. Bar graphs are plotted as mean ± SEM. Mann–Whitney U test (E), one-way ANOVA (F). See Table 1.
β Actin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β actin  (R&D Systems)
93
R&D Systems β actin
TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. <t>β-actin</t> was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.
β Actin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β actin mab8929  (R&D Systems)
96
R&D Systems β actin mab8929
TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. <t>β-actin</t> was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.
β Actin Mab8929, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hk200550  (OriGene)
93
OriGene hk200550
TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. <t>β-actin</t> was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.
Hk200550, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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beta actin  (OriGene)
94
OriGene beta actin
TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. <t>β-actin</t> was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.
Beta Actin, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β actin antibody  (R&D Systems)
92
R&D Systems β actin antibody
TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. <t>β-actin</t> was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.
β Actin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human beta actin actb elisa kit  (Cusabio)
93
Cusabio human beta actin actb elisa kit
Differentially expressed proteins and their expression levels quantified by iTRAQ-2DLC-MS/MS.
Human Beta Actin Actb Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhodamine labeled actin  (Cytoskeleton Inc)
92
Cytoskeleton Inc rhodamine labeled actin
Differentially expressed proteins and their expression levels quantified by iTRAQ-2DLC-MS/MS.
Rhodamine Labeled Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rea1148  (Miltenyi Biotec)
93
Miltenyi Biotec rea1148
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Rea1148, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Transcriptional reprogramming of neuronal metabolism during glucose deprivation. (A) Schematic for glucose deprivation of rat cortical neurons. A subset of ∼250 genes significantly upregulated during glucose deprivation (P value <0.01) were selected for pathway analysis using annotated gene sets from the Molecular Signatures Database (MSigDB). n = 3 cortical samples from one rat litter. (B) Enrichment of genes in MSigDB Reactome pathways with adjusted P value <0.05. (C) Enrichment of the target genes of the CREB transcription factor in glucose-deprived neurons (adjusted P value <0.05). The target gene Pgc1α (PPARGC1A) is highlighted in red. (D) Immunostaining of cortical neuronal cultures (treated as in A) with an anti-phospho-CREB antibody and Hoechst nuclear stain. Arrowheads denote pCREB-positive nuclei. (E) Fraction of nuclei with positive p-CREB staining in fields of view (FOV), determined as described in Materials and methods. % total ± SEM: +glucose (3 h), 14.87 ± 2.29, −glucose (3 h), 27.41 ± 3.3. n = 17–22 FOVs. (F) Relative mRNA expression of Pgc1α in neuronal cultures treated as in A, with or without the AMPK inhibitor, dorsomorphin. Values are normalized to β-actin mRNA and expressed relative to the +glucose condition. Average normalized mRNA level ± SEM: −glucose (1 h), 2.83 ± 0.42; −glucose (3 h), 3.67 ± 0.46; −glucose + dorsomorphin (3 h), 1.32 ± 0.22. n = 3–10 cortical samples. Bar graphs are plotted as mean ± SEM. Mann–Whitney U test (E), one-way ANOVA (F). See Table 1.

Journal: The Journal of cell biology

Article Title: Sirtuin3 ensures the metabolic plasticity of neurotransmission during glucose deprivation.

doi: 10.1083/jcb.202305048

Figure Lengend Snippet: Figure 1. Transcriptional reprogramming of neuronal metabolism during glucose deprivation. (A) Schematic for glucose deprivation of rat cortical neurons. A subset of ∼250 genes significantly upregulated during glucose deprivation (P value <0.01) were selected for pathway analysis using annotated gene sets from the Molecular Signatures Database (MSigDB). n = 3 cortical samples from one rat litter. (B) Enrichment of genes in MSigDB Reactome pathways with adjusted P value <0.05. (C) Enrichment of the target genes of the CREB transcription factor in glucose-deprived neurons (adjusted P value <0.05). The target gene Pgc1α (PPARGC1A) is highlighted in red. (D) Immunostaining of cortical neuronal cultures (treated as in A) with an anti-phospho-CREB antibody and Hoechst nuclear stain. Arrowheads denote pCREB-positive nuclei. (E) Fraction of nuclei with positive p-CREB staining in fields of view (FOV), determined as described in Materials and methods. % total ± SEM: +glucose (3 h), 14.87 ± 2.29, −glucose (3 h), 27.41 ± 3.3. n = 17–22 FOVs. (F) Relative mRNA expression of Pgc1α in neuronal cultures treated as in A, with or without the AMPK inhibitor, dorsomorphin. Values are normalized to β-actin mRNA and expressed relative to the +glucose condition. Average normalized mRNA level ± SEM: −glucose (1 h), 2.83 ± 0.42; −glucose (3 h), 3.67 ± 0.46; −glucose + dorsomorphin (3 h), 1.32 ± 0.22. n = 3–10 cortical samples. Bar graphs are plotted as mean ± SEM. Mann–Whitney U test (E), one-way ANOVA (F). See Table 1.

Article Snippet: The following primary antibodies were used: Sirt3 (1:1,000, 5490S; Cell Signaling Technology, rabbit, reactivity for human, mouse, and rat), anti-acetyl-lysine (1:1,000, 9441S; Cell Signaling Technology rabbit, all species expected), ATPase5β (1:1,000, HPA001520; Sigma-Aldrich, rabbit, reactivity for rat, mouse, and humans), β-actin (1:5,000; HCA147P; Bio-Rad, Human Combinatorial Antibody Library/ HuCAL, reactivity for rat, mouse, and humans), α-tubulin (1:2,000, T6074; Sigma-Aldrich, mouse, reactivity for mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, and kangaroo rat).

Techniques: Immunostaining, Staining, Expressing, MANN-WHITNEY

Figure 2. Glucose deprivation stimulates neuronal Sirt3 expression and deacetylation of mitochondrial proteins. (A) Relative Sirt3 mRNA expression in control and glucose-deprived neu- rons. Values are normalized to β-actin mRNA and expressed relative to control (+glucose). n = 3–10 cortical samples. Average normalized mRNA level ± SEM: −glucose (1 h), 2.58 ± 0.62; −glucose (3 h), 2.20 ± 0.27; −glucose + dorsomorphin (3 h), 1.12 ± 0.08. n = 3–11 cortical samples. (B) Relative Sirt3 mRNA expression in cultures transduced with adenoviral particles encoding GFP (control) and GFP-PGC1α, normalized to 18s rRNA and ex- pressed relative to control. Average normalized mRNA level ± SEM: GFP-PGC1α, 1.93 ± 0.30. n = 16 cortical samples/condition. (C) Immunoblotting of Sirt3 protein expression in cortical neurons with antibodies against Sirt3 and the cytosolic and mitochondrial controls, β-actin, and ATPase5β, respectively. (D) Sirt3 band intensity normalized to the ATPase5β and expressed relative to control. Average normalized Sirt3 band intensity ± SEM: +glucose, 0.99 ± 0.05; −glucose, 1.74 ± 0.16. n = 7 cortical samples/condition. (E) A paradigm for the analysis of Sirt3 expression in hippocampi of mice fed ad libitum (ad lib) or alternate-day fasted for 6 mo (ADF). Schematic created with https:// biorender.com. (F) Immunoblotting of Sirt3 protein in mouse hippocampal lysates. (G) Sirt3 band intensity normalized to the ATPase5β band and expressed relative to the ad lib mice. Average normalized Sirt3 band intensity ± SEM: ad lib, 1 ± 0.24; ADF (6 mo), 1.88 ± 0.19. n = 6 mice/condition. (H) Mitochondrial and cytosolic fractions isolated from cortical neuronal cul- tures maintained for 3 h with (+) or without (−) glucose. High and low exposures were 60 and 25 s, respectively. (I) Intensity of lysine acetylation bands normalized to α-tubulin or ATPase5β plot- ted relative to control. Average normalized Ac-K intensity: cytosolic fraction (−glucose), 1.21 ± 0.15; mitochondrial fraction (−glucose), 0.66 ± 0.08. n = 5 cortical samples. One-way ANOVA (A), two- tailed, unpaired t test (B), Mann–Whitney U test (D and G), one sample t test (I). See Table 1. Source data are available for this figure: SourceData F2.

Journal: The Journal of cell biology

Article Title: Sirtuin3 ensures the metabolic plasticity of neurotransmission during glucose deprivation.

doi: 10.1083/jcb.202305048

Figure Lengend Snippet: Figure 2. Glucose deprivation stimulates neuronal Sirt3 expression and deacetylation of mitochondrial proteins. (A) Relative Sirt3 mRNA expression in control and glucose-deprived neu- rons. Values are normalized to β-actin mRNA and expressed relative to control (+glucose). n = 3–10 cortical samples. Average normalized mRNA level ± SEM: −glucose (1 h), 2.58 ± 0.62; −glucose (3 h), 2.20 ± 0.27; −glucose + dorsomorphin (3 h), 1.12 ± 0.08. n = 3–11 cortical samples. (B) Relative Sirt3 mRNA expression in cultures transduced with adenoviral particles encoding GFP (control) and GFP-PGC1α, normalized to 18s rRNA and ex- pressed relative to control. Average normalized mRNA level ± SEM: GFP-PGC1α, 1.93 ± 0.30. n = 16 cortical samples/condition. (C) Immunoblotting of Sirt3 protein expression in cortical neurons with antibodies against Sirt3 and the cytosolic and mitochondrial controls, β-actin, and ATPase5β, respectively. (D) Sirt3 band intensity normalized to the ATPase5β and expressed relative to control. Average normalized Sirt3 band intensity ± SEM: +glucose, 0.99 ± 0.05; −glucose, 1.74 ± 0.16. n = 7 cortical samples/condition. (E) A paradigm for the analysis of Sirt3 expression in hippocampi of mice fed ad libitum (ad lib) or alternate-day fasted for 6 mo (ADF). Schematic created with https:// biorender.com. (F) Immunoblotting of Sirt3 protein in mouse hippocampal lysates. (G) Sirt3 band intensity normalized to the ATPase5β band and expressed relative to the ad lib mice. Average normalized Sirt3 band intensity ± SEM: ad lib, 1 ± 0.24; ADF (6 mo), 1.88 ± 0.19. n = 6 mice/condition. (H) Mitochondrial and cytosolic fractions isolated from cortical neuronal cul- tures maintained for 3 h with (+) or without (−) glucose. High and low exposures were 60 and 25 s, respectively. (I) Intensity of lysine acetylation bands normalized to α-tubulin or ATPase5β plot- ted relative to control. Average normalized Ac-K intensity: cytosolic fraction (−glucose), 1.21 ± 0.15; mitochondrial fraction (−glucose), 0.66 ± 0.08. n = 5 cortical samples. One-way ANOVA (A), two- tailed, unpaired t test (B), Mann–Whitney U test (D and G), one sample t test (I). See Table 1. Source data are available for this figure: SourceData F2.

Article Snippet: The following primary antibodies were used: Sirt3 (1:1,000, 5490S; Cell Signaling Technology, rabbit, reactivity for human, mouse, and rat), anti-acetyl-lysine (1:1,000, 9441S; Cell Signaling Technology rabbit, all species expected), ATPase5β (1:1,000, HPA001520; Sigma-Aldrich, rabbit, reactivity for rat, mouse, and humans), β-actin (1:5,000; HCA147P; Bio-Rad, Human Combinatorial Antibody Library/ HuCAL, reactivity for rat, mouse, and humans), α-tubulin (1:2,000, T6074; Sigma-Aldrich, mouse, reactivity for mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, and kangaroo rat).

Techniques: Expressing, Control, Transduction, Western Blot, Isolation, Two Tailed Test, MANN-WHITNEY

TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. β-actin was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.

Journal: Molecular Medicine Reports

Article Title: Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis

doi: 10.3892/mmr.2019.10631

Figure Lengend Snippet: TNF-α mediates TLR expression and NF-κB p65 signaling in PBMCs. (A) Western blotting was performed to detect the protein levels of TLR3, TLR4, TLR5, p65 and p-p65. Protein expression of (B) TLR3, (C) TLR4, (D) TLR5 and (E) p-p65/p65 presented as bar diagrams. β-actin was used as an internal control. *P<0.05, **P<0.01 vs. control. NF-κB, nuclear factor-κB; p-, phosphorylated; PBMC, peripheral blood mononuclear cell; TLR, Toll-like receptor; TNF-α, tumor necrosis factor-α.

Article Snippet: The membranes were blocked in 5% dried skimmed milk in TBS buffer at 37°C for 1 h and incubated overnight at 4°C with antibodies against TLR3 (1:700; ab62566, Abcam), TLR4 (1:600; ab13556, Abcam), TLR5 (1:800; ab62460, Abcam), p65 (1:1,000; ab32536, Abcam), phosphorylated (p)-p65 (1:600; ab86299, Abcam) and β-actin (1:1,000; MAB8969, R&D Systems, Inc.).

Techniques: Expressing, Western Blot, Control

Differentially expressed proteins and their expression levels quantified by iTRAQ-2DLC-MS/MS.

Journal: Frontiers in Endocrinology

Article Title: Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans

doi: 10.3389/fendo.2021.830691

Figure Lengend Snippet: Differentially expressed proteins and their expression levels quantified by iTRAQ-2DLC-MS/MS.

Article Snippet: The human prostatic acid phosphatase (PAP, ACPP) ELISA kit (ab267802; Abcam, Cambridge, MA, USA; detection limit, 9.73 pg/mL; SwissProt: P15309), human CD5 antigen-like (CD5L) ELISA kit (ab213760; Abcam, Cambridge, MA, USA; detection limit, < 10 pg/mL; SwissProt: O43866), human alpha 2 macroglobulin (A2M) ELISA kit (ab108883; Abcam, Cambridge; detection limit, 1.25 μg/mL; SwissProt: P01023), human peptidyl-prolyl cis-trans isomerase A (PPIA) ELISA kit (CSB-E09920h; CUSABIO Co., Wuhan, China; detection limit, 0.78 ng/mL; SwissProt: P62937), and human beta-actin (ACTB) ELISA kit (CSB-E13298h; CUSABIO Co.; detection limit, 0.078 ng/mL; SwissProt: P60709) were used to detect protein levels in follicular fluid.

Techniques: Expressing, Multiplex sample analysis

Enzyme-linked immunosorbent assay results of candidate proteins in MII and Gv oocytes in follicular fluid. (A) Prostatic acid phosphatase (ACPP); (B) CD5 antigen-like (CD5L); (C) Alpha 2 macroglobulin (A2M); (D) Peptidyl-prolyl cis-trans isomerase A (PPIA); (E) Beta-actin (ACTB). *** P < 0.0001.

Journal: Frontiers in Endocrinology

Article Title: Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans

doi: 10.3389/fendo.2021.830691

Figure Lengend Snippet: Enzyme-linked immunosorbent assay results of candidate proteins in MII and Gv oocytes in follicular fluid. (A) Prostatic acid phosphatase (ACPP); (B) CD5 antigen-like (CD5L); (C) Alpha 2 macroglobulin (A2M); (D) Peptidyl-prolyl cis-trans isomerase A (PPIA); (E) Beta-actin (ACTB). *** P < 0.0001.

Article Snippet: The human prostatic acid phosphatase (PAP, ACPP) ELISA kit (ab267802; Abcam, Cambridge, MA, USA; detection limit, 9.73 pg/mL; SwissProt: P15309), human CD5 antigen-like (CD5L) ELISA kit (ab213760; Abcam, Cambridge, MA, USA; detection limit, < 10 pg/mL; SwissProt: O43866), human alpha 2 macroglobulin (A2M) ELISA kit (ab108883; Abcam, Cambridge; detection limit, 1.25 μg/mL; SwissProt: P01023), human peptidyl-prolyl cis-trans isomerase A (PPIA) ELISA kit (CSB-E09920h; CUSABIO Co., Wuhan, China; detection limit, 0.78 ng/mL; SwissProt: P62937), and human beta-actin (ACTB) ELISA kit (CSB-E13298h; CUSABIO Co.; detection limit, 0.078 ng/mL; SwissProt: P60709) were used to detect protein levels in follicular fluid.

Techniques: Enzyme-linked Immunosorbent Assay

Proteins expression levels change between diminished ovarian response (DOR) patients and controls (CON) in follicular fluid and cumulus cells. (A) Prostatic acid phosphatase (ACPP) and (B) CD5 antigen-like (CD5L) levels in follicular fluid were analyzed by enzyme-linked immunosorbent assay. (C) ACPP and (D) CD5L levels in cumulus cells were analyzed by western-blot. * P < 0.05; ** P < 0.01; *** P < 0.0001.

Journal: Frontiers in Endocrinology

Article Title: Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans

doi: 10.3389/fendo.2021.830691

Figure Lengend Snippet: Proteins expression levels change between diminished ovarian response (DOR) patients and controls (CON) in follicular fluid and cumulus cells. (A) Prostatic acid phosphatase (ACPP) and (B) CD5 antigen-like (CD5L) levels in follicular fluid were analyzed by enzyme-linked immunosorbent assay. (C) ACPP and (D) CD5L levels in cumulus cells were analyzed by western-blot. * P < 0.05; ** P < 0.01; *** P < 0.0001.

Article Snippet: The human prostatic acid phosphatase (PAP, ACPP) ELISA kit (ab267802; Abcam, Cambridge, MA, USA; detection limit, 9.73 pg/mL; SwissProt: P15309), human CD5 antigen-like (CD5L) ELISA kit (ab213760; Abcam, Cambridge, MA, USA; detection limit, < 10 pg/mL; SwissProt: O43866), human alpha 2 macroglobulin (A2M) ELISA kit (ab108883; Abcam, Cambridge; detection limit, 1.25 μg/mL; SwissProt: P01023), human peptidyl-prolyl cis-trans isomerase A (PPIA) ELISA kit (CSB-E09920h; CUSABIO Co., Wuhan, China; detection limit, 0.78 ng/mL; SwissProt: P62937), and human beta-actin (ACTB) ELISA kit (CSB-E13298h; CUSABIO Co.; detection limit, 0.078 ng/mL; SwissProt: P60709) were used to detect protein levels in follicular fluid.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Immunophenotyping panel for multiplexed tissue imaging of cancer.

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: β-Actin , REA1148 , 50 , 130-120-276 , FITC , Miltenyi Biotec.

Techniques: Imaging