Journal: Scientific Reports

Article Title: 3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells

doi: 10.1038/s41598-024-54912-1

Figure Lengend Snippet: The cell proliferation and morphology of 3D hMSCs in biomaterial hydrogels. ( A ) The proliferation capacity of MSCs in various biomaterial hydrogel cultures for 15 days, as determined by the resazurin assay. ( B ) The proliferation rate of 2D and 3D AL-HA hydrogels during 15 days of culture and ( C ) The morphology of MSCs in 2D and 3D biomaterial hydrogels at day 7 of culture.

Article Snippet: The Umbilical cord-derived hMSCs (hUC-MSCs) was derived from I Wellness Co., Ltd. (Mueng, Nakhon Ratchasima, Thailand) by a healthy female donor aged 39 years who obtained written informed consent in accordance with the Human Research Ethics Committee under ethically acceptable terms of Suranaree University of Technology (EC63-06).

Techniques: Resazurin Assay

Journal: Scientific Reports

Article Title: 3D culture of alginate-hyaluronic acid hydrogel supports the stemness of human mesenchymal stem cells

doi: 10.1038/s41598-024-54912-1

Figure Lengend Snippet: The characterization of hMSCs. The expression of MSCs surface markers. Representative flow cytometry analyses for the expression of the positive markers, ( A ) Mouse IgG1 Anti-Human CD73-PeCY7, ( B ) Mouse IgG2A Anti-Human CD90-FITC and ( C ) Mouse IgG1 Anti-Human CD105-PE, the negative Markers, ( D ) Mouse IgG1 Anti-Human CD34-PE and ( E ) Mouse IgG1 Anti-Human CD45-PC5. ( F ) Trilineage differentiation of hMSCs: osteogenic, chondrogenic, and adipogenic differentiation.

Article Snippet: The Umbilical cord-derived hMSCs (hUC-MSCs) was derived from I Wellness Co., Ltd. (Mueng, Nakhon Ratchasima, Thailand) by a healthy female donor aged 39 years who obtained written informed consent in accordance with the Human Research Ethics Committee under ethically acceptable terms of Suranaree University of Technology (EC63-06).

Techniques: Expressing, Flow Cytometry

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: Collection of human umbilical cord mesenchymal stem. A: Serum-free human umbilical cord mesenchymal stem, scale bar = 200 μm; B: Serum-cultured human umbilical cord mesenchymal stem cells, scale bar = 200 μm. N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: Average body weight of mice in each group. The weight of each group of mice showed an upward trend after surgical modeling. N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells; HA: Hyaluronic acid.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: Reduced interleukin-6 and interleukin-1β expression in the experimental group. A: Baseline analysis: There was no statistically significant difference in the initial levels of interleukin (IL)-1β among the groups of animals; B: Baseline analysis: There was no statistically significant difference in the initial levels of IL-6 among the groups of animals; C: Model validation: The level of IL-1β in the model control group was significantly higher than that in the sham surgery group (blank control group); D: Model validation: The level of IL-6 in the model control group was significantly higher than that in the sham surgery group (blank control group); E: Experimental endpoints: The level of IL-1β in the experimental group was significantly higher than that in the model control group; F: Experimental endpoints: The level of IL-6 in the experimental group was significantly higher than that in the model control group. a P < 0.05, comparison with the sham operated group; b P < 0.01, comparison with the sham operated group; c P < 0.05, comparison with the model control group; d P < 0.01, comparison with the model control group. N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells; IL: Interleukin.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Expressing, Biomarker Discovery, Control, Comparison, Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: The experimental group favored the repair of cartilage tissue. A and B: Model validation: In the control group, the integrity of the articular cartilage was disrupted, the cartilage surface became rough and uneven, the synovial tissue in the joint cavity proliferated significantly, and the number of osteoclasts in the bone marrow cavity increased; C: Experimental endpoints: The number of chondrocytes in the treatment group was significantly increased compared to the model control group; D: Experimental endpoints: The Mankin score of the model control group was significantly higher than that of the sham surgery group (blank control group). b P < 0.01, comparison with the sham-operated group. N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Biomarker Discovery, Control, Comparison, Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: X-ray. Radiograph of the knee joint before the anatomical experiment. The experimental group can mice with reduced formation of osteoid in the knee joint. N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: Histological images of cartilage tissue of knee joints of each group. The collagen II staining was strongest in the sham surgery group, weakest in the model control group, weakest in matrix metalloproteinase-1 and matrix metalloproteinase-13 staining in the sham surgery group (blank control group), and strongest in the model control group. MMP: Matrix metalloproteinase; N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Staining, Control, Cell Culture

Journal: World Journal of Stem Cells

Article Title: Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice

doi: 10.4252/wjsc.v16.i11.944

Figure Lengend Snippet: Western bolt. A: Western bolt original picture; B: The model control group had the lowest expression level of collagen II, while the blank control group had the highest expression level; C: The model control group had the highest expression level of matrix metalloproteinase-1, while the blank control group had the lowest expression level; D: The model control group had the highest expression level of matrix metalloproteinase-13, while the blank control group had the lowest expression level. b P < 0.01, comparison with the sham-operated group; c P < 0.05, comparison with the modeled control. MMP: Matrix metalloproteinase; N-hUCMSCs: Serum-free human umbilical cord mesenchymal stems; S-hUCMSCs: Serum-cultured human umbilical cord mesenchymal stem cells.

Article Snippet: P3 generation hUCMSCs were purchased from Guangxi Huaren & Sai Biotechnology Co., Ltd., divided into serum (10% fetal bovine serum) culture and, serum-free culture groups, and passaged to P5 (Figure ). hUCMSCs from the 5 th generation were used for all experiments.

Techniques: Western Blot, Control, Expressing, Comparison, Cell Culture

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: ATRA pretreatment improves the protective effect of MSCs on renal function and pathologic damage in I/R-induced AKI. a GFR assessment using transcutaneous clearance of FITC-sinistrin ( n = 5). b, c SCr and BUN levels ( n = 6). d Semi-quantitative analysis of histological features ( n = 6). e Representative images of PAS staining on renal tissue sections in different groups of mice. Scale bars, 200 μm and 50 μm in scanned and zoom in figures, respectively. f Representative immunofluorescence staining of KIM-1. Scale bars, 200 μm and 50 μm in scanned and zoom in figures, respectively. g, h Representative western blot images and quantitative analysis of KIM-1 expression in kidney tissues ( n = 3). Data are presented as the means ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Staining, Immunofluorescence, Western Blot, Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: ATRA regulates the expression of HA by regulating the binding of the transcription factors RARα/RXRγ to HAS2 gene. a Viability of MSCs after ATRA pretreatment ( n = 6). b Volcano plot of showing the differentially expressed genes in ATRA-MSCs versus DMSO-MSCs ( n = 3). Red dots indicate significant up-regulation, green dots indicate significant down-regulation, and gray dots indicate insignificant differences. c Scatter plot of GO enrichment analysis ( n = 3). The number of enriched differential genes can be observed by the dot size, and the significance of the enrichment is indicated by the dot color intensity. d The mRNA expression of HAS1, HAS2 and HAS3 ( n = 3). e Transfection efficiency of different siRNA ( n = 5). f The concentration of HA in the MSCs culture supernatant ( n = 4). g RARα/RXRγ binding motif logo generated by the WebLogo tool. h Schematic representation of possible binding sites for the transcription factor RARα/RXRγ in the HAS2 promoter region. i ChIP-qPCR analysis of HAS2 target promotor regions ( n = 3). Data are presented as the means ± SD. **** P < 0.0001, ** P < 0.01

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Expressing, Binding Assay, Transfection, Concentration Assay, Generated, ChIP-qPCR

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: ATRA enhances the anti-inflammatory, anti-apoptosis and pro-proliferation effects of MSCs on H/R-induced HK-2 cells in vitro. a-g Representative western blot images and quantitative analysis of TNF-a, IL-6, Bax, Bcl-2, and cleaved caspase-3 in HK-2 cells ( n = 3). h, I Representative western blot images and quantitative analysis of PCNA in HK-2 cells ( n = 3). Data are presented as the means ± SD. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: In Vitro, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: ATRA enhances the anti-inflammatory, anti-apoptosis and pro-proliferation effects of MSCs on I/R-induced AKI in vivo. a TNF-a mRNA expression level ( n = 3). b IL-6 mRNA expression level ( n = 3). c IL-1β mRNA expression level ( n = 3). d-j Representative western blot images and quantitative analysis of TNF-a, IL-6, Bax, Bcl-2, cleaved caspase-3 and PCNA in kidney tissues ( n = 3). k Apoptotic cells were labeled with the TUNEL assay kit (red) with nuclei counterstained with DAPI (blue) in kidney sections. Scale bar, 50 μm. l Quantitative analysis of the percentage of apoptotic cells ( n = 3). m Representative images of PCNA in kidney tissues. Scale bar, 40 μm. n Quantitative analysis of PCNA-positive cells ( n = 3). Data are presented as the means ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: In Vivo, Expressing, Western Blot, Labeling, TUNEL Assay

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: Inhibition of the HA/CD44 axis reverses the anti-inflammatory, anti-apoptotic and pro-proliferative effects of ATRA-MSCs. a, c-i Western blot images and quantitative densitometry analysis of TNF-a, IL-6, Bax, Bcl-2 and cleaved caspase-3 in HK-2 cells ( n = 3). b, j-l Western blot images and quantitative densitometry analysis of p-AKT and total AKT in HK-2 cells ( n = 3). Data are presented as the means ± SD. **** P < 0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Inhibition, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: All-trans retinoic acid pretreatment of mesenchymal stem cells enhances the therapeutic effect on acute kidney injury

doi: 10.1186/s12964-024-01671-1

Figure Lengend Snippet: Schematic illustration of ATRA promoted HA production by MSCs and activated the PI3K/AKT pathway by binding to CD44 in renal tubular epithelial cells, thereby improving renal repair after AKI

Article Snippet: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were acquired from the Beijing Zhongyuan Company (Beijing, China).

Techniques: Binding Assay

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: Expansion of batch-cultured hUCMSCs in 3D spinner flask. ( A ) Representative images of hUCMSCs on Cytodex3 cultured in 3D spinner flask with different rotating speeds (35 rpm, 45 rpm, 55 rpm). ( B ) Cell density changes when culturing the P3 hUCMSCs for 1–7 days. ( C ) Cell adherence rate on day 1 after seeding. ( D ) Cell viability during expansion of hUCMSCs. Data expressed as the mean ± standard deviation ( n = 3 per group, per time point).

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Cell Culture, Standard Deviation

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: Effects of agitation speeds on the proliferation of hUCMSCs cultured in 200 mL spinner flask.

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Cell Culture

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: Cell metabolism characterization of batch-cultured hUCMSCs in 200 mL spinner flask. metabolite concentration assessment in the medium, including ( A ) glucose, ( B ) glutamine, ( C ) lactate and ( D ) ammonia. ( E ) Characterization of the hUCMSCs surface markers by flow cytometry using positive markers (FITC CD90, PE CD105 and CD73) and negative ones (APC CD14, CD19, CD34, CD45 and HLA-DR), where the gray and colored peak respectively represent the control and experimental group.

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Cell Culture, Concentration Assay, Flow Cytometry, Control

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: ( A ) Morphology and distribution of batch-cultured hUCMSCs on microcarriers at different time points (Day 1 to Day 7) cultured in both BioStar 1.5c and the spinner flask. ( B , C ) Cell viability and density during expansion of hUCMSCs. Data expressed as the mean ± standard deviation ( n = 4 per group, per time point).

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Cell Culture, Standard Deviation

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: Effects of the two culture methods on the proliferation of hUCMSCs.

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques:

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: Metabolite concentration assessment of the batch-cultured hUCMSCs medium from BioStar 1.5c and the spinner flask, including glucose ( A ), glutamine ( B ), lactate ( C ) and ammonia ( D ). ( E ) Characterization of the hUCMSCs surface markers by flow cytometry using positive markers (FITC CD90, PE CD105 and CD73) and negative ones (APC CD14, CD19, CD34, CD45 and HLA-DR), where the gray and colored peak respectively represent the control and experimental group.

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Concentration Assay, Cell Culture, Flow Cytometry, Control

Journal: Bioengineering

Article Title: Large-Scale Expansion of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Stirred Suspension Bioreactor Enabled by Computational Fluid Dynamics Modeling

doi: 10.3390/bioengineering9070274

Figure Lengend Snippet: ( A ) Metabolite concentration assessment of the hUCMSCs medium from BioStar 1.5c when batch or fed-batch culture, including glucose, glutamine, lactate and ammonia. ( B , C ) Cell density and viability. Adipogenic ( D ) and osteogenic ( E ) differentiation evaluation of the expanded hUCMSCs by fed-batch culture in BioStar 1.5c. Surface markers characterization of the expanded hUCMSCs by fed-batch culture in BioStar 1.5c by flow cytometry ( F ), where the gray and colored peak respectively represent the control and experimental group.

Article Snippet: As shown in D–F, the expanded hUCMSCs by fed-batch culture in BioStar 1.5c maintained the adipogenic and osteogenic differentiation potential and still expressed the characteristic surface markers of hUCMSCs in a high population (>98%), indicating the ignorable MSCs variation.

Techniques: Concentration Assay, Flow Cytometry, Control